Advanced

Glutamate detection from nerve cells using a planar electrodes array integrated in a microtiter plate

Castillo Leon, Jaime LU ; Blochl, A; Dennison, S; Schuhmann, W and Csöregi, Elisabeth LU (2005) In Biosensors & Bioelectronics 20(10). p.2116-2119
Abstract
There is an increasing interest in new strategies for replacing animal tests in research. The use of cell cultures and integrated electrodes is seen as a promising alternative that could potentially solve this problem. In this work, we present a L-glutamate sensor based on a bienzyme redox hydrogel, capable of detecting the release of this excitatory neurotransmitter from adherently growing cells upon stimulation. The low working potential required for the operation of the sensor decreases the possibility of interference by easily oxidizable compounds always present in complex biological samples. A low detection limit of 0.5 mu M L-glutamate, a response time of about 35 s, and a linear range of up to 60 mu M are the main characteristics of... (More)
There is an increasing interest in new strategies for replacing animal tests in research. The use of cell cultures and integrated electrodes is seen as a promising alternative that could potentially solve this problem. In this work, we present a L-glutamate sensor based on a bienzyme redox hydrogel, capable of detecting the release of this excitatory neurotransmitter from adherently growing cells upon stimulation. The low working potential required for the operation of the sensor decreases the possibility of interference by easily oxidizable compounds always present in complex biological samples. A low detection limit of 0.5 mu M L-glutamate, a response time of about 35 s, and a linear range of up to 60 mu M are the main characteristics of the sensor. The system has been successfully employed to monitor the release of L-glutamate from HN10 and C6 cells upon stimulation with K+-ions. The developed integrated electrochemical platform will be used in future for drug screening and potentially for replacing animal models in neurological experiments. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microliter, biosensor, cell culture, L-glutamate, electrode array, plate, screen printed electrodes
in
Biosensors & Bioelectronics
volume
20
issue
10
pages
2116 - 2119
publisher
Elsevier
external identifiers
  • wos:000227845700029
  • pmid:15741083
  • scopus:14644416517
ISSN
1873-4235
DOI
10.1016/j.bios.2004.09.013
language
English
LU publication?
yes
id
9cb75383-d08e-4a92-baf9-250f4fa1d9ef (old id 248058)
date added to LUP
2007-08-14 08:56:19
date last changed
2017-09-03 04:45:16
@article{9cb75383-d08e-4a92-baf9-250f4fa1d9ef,
  abstract     = {There is an increasing interest in new strategies for replacing animal tests in research. The use of cell cultures and integrated electrodes is seen as a promising alternative that could potentially solve this problem. In this work, we present a L-glutamate sensor based on a bienzyme redox hydrogel, capable of detecting the release of this excitatory neurotransmitter from adherently growing cells upon stimulation. The low working potential required for the operation of the sensor decreases the possibility of interference by easily oxidizable compounds always present in complex biological samples. A low detection limit of 0.5 mu M L-glutamate, a response time of about 35 s, and a linear range of up to 60 mu M are the main characteristics of the sensor. The system has been successfully employed to monitor the release of L-glutamate from HN10 and C6 cells upon stimulation with K+-ions. The developed integrated electrochemical platform will be used in future for drug screening and potentially for replacing animal models in neurological experiments.},
  author       = {Castillo Leon, Jaime and Blochl, A and Dennison, S and Schuhmann, W and Csöregi, Elisabeth},
  issn         = {1873-4235},
  keyword      = {microliter,biosensor,cell culture,L-glutamate,electrode array,plate,screen printed electrodes},
  language     = {eng},
  number       = {10},
  pages        = {2116--2119},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Glutamate detection from nerve cells using a planar electrodes array integrated in a microtiter plate},
  url          = {http://dx.doi.org/10.1016/j.bios.2004.09.013},
  volume       = {20},
  year         = {2005},
}