Microstructural heterogeneity of the collagenous network in the loaded and unloaded periodontal ligament and its biomechanical implications
Zhong, Jingxiao ; Pierantoni, Maria LU
; Weinkamer, Richard
; Brumfeld, Vlad
; Zheng, Keke
; Chen, Junning
; Swain, Michael V.
; Weiner, Steve
and Li, Qing
(2021)
In Journal of Structural Biology
213(3).
- Abstract
The periodontal ligament (PDL) is a highly heterogeneous fibrous connective tissue and plays a critical role in distributing occlusal forces and regulating tissue remodeling. Its mechanical properties are largely determined by the extracellular matrix, comprising a collagenous fiber network interacting with the capillary system as well as interstitial fluid containing proteoglycans. While the phase-contrast micro-CT technique has portrayed the 3D microscopic heterogeneity of PDL, the topological parameters of its network, which is crucial to understanding the multiscale constitutive behavior of this tissue, has not been characterized quantitatively. This study aimed to provide new understanding of such microscopic heterogeneity of the... (More)
The periodontal ligament (PDL) is a highly heterogeneous fibrous connective tissue and plays a critical role in distributing occlusal forces and regulating tissue remodeling. Its mechanical properties are largely determined by the extracellular matrix, comprising a collagenous fiber network interacting with the capillary system as well as interstitial fluid containing proteoglycans. While the phase-contrast micro-CT technique has portrayed the 3D microscopic heterogeneity of PDL, the topological parameters of its network, which is crucial to understanding the multiscale constitutive behavior of this tissue, has not been characterized quantitatively. This study aimed to provide new understanding of such microscopic heterogeneity of the PDL with quantifications at both tissue and collagen network levels in a spatial manner, by combining phase-contrast micro-CT imaging and a purpose-built image processing algorithm for fiber analysis. Both variations within a PDL and among the PDL with different shapes, i.e. round-shaped and kidney-shaped PDLs, are described in terms of tissue thickness, fiber distribution, local fiber densities, and fiber orientation (namely azimuthal and elevation angles). Furthermore, the tissue and collagen fiber network responses to mechanical loading were evaluated in a similar manner. A 3D helical alignment pattern was observed in the fiber network, which appears to regulate and adapt a screw-like tooth motion under occlusion. The microstructural heterogeneity quantified here allows development of sample-specific constitutive models to characterize the PDL's functional and pathological loading responses, thereby providing a new multiscale framework for advancing our knowledge of this complex limited mobility soft-hard tissue interface.
(Less)
- author
- Zhong, Jingxiao
; Pierantoni, Maria
LU
; Weinkamer, Richard
; Brumfeld, Vlad
; Zheng, Keke
; Chen, Junning
; Swain, Michael V.
; Weiner, Steve
and Li, Qing
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fast-Fourier transform, Fibrous network quantification, Loading response, Phase-contrast micro-CT, Soft tissue heterogeneity
- in
- Journal of Structural Biology
- volume
- 213
- issue
- 3
- article number
- 107772
- publisher
- Elsevier
- external identifiers
-
- scopus:85111519343
- pmid:34311076
- ISSN
- 1047-8477
- DOI
- 10.1016/j.jsb.2021.107772
- language
- English
- LU publication?
- yes
- id
- 24841d33-a8b8-47a9-a671-03d4cafc8d22
- date added to LUP
- 2021-08-24 13:25:21
- date last changed
- 2024-06-01 14:26:52
@article{24841d33-a8b8-47a9-a671-03d4cafc8d22,
abstract = {{<p>The periodontal ligament (PDL) is a highly heterogeneous fibrous connective tissue and plays a critical role in distributing occlusal forces and regulating tissue remodeling. Its mechanical properties are largely determined by the extracellular matrix, comprising a collagenous fiber network interacting with the capillary system as well as interstitial fluid containing proteoglycans. While the phase-contrast micro-CT technique has portrayed the 3D microscopic heterogeneity of PDL, the topological parameters of its network, which is crucial to understanding the multiscale constitutive behavior of this tissue, has not been characterized quantitatively. This study aimed to provide new understanding of such microscopic heterogeneity of the PDL with quantifications at both tissue and collagen network levels in a spatial manner, by combining phase-contrast micro-CT imaging and a purpose-built image processing algorithm for fiber analysis. Both variations within a PDL and among the PDL with different shapes, i.e. round-shaped and kidney-shaped PDLs, are described in terms of tissue thickness, fiber distribution, local fiber densities, and fiber orientation (namely azimuthal and elevation angles). Furthermore, the tissue and collagen fiber network responses to mechanical loading were evaluated in a similar manner. A 3D helical alignment pattern was observed in the fiber network, which appears to regulate and adapt a screw-like tooth motion under occlusion. The microstructural heterogeneity quantified here allows development of sample-specific constitutive models to characterize the PDL's functional and pathological loading responses, thereby providing a new multiscale framework for advancing our knowledge of this complex limited mobility soft-hard tissue interface.</p>}},
author = {{Zhong, Jingxiao and Pierantoni, Maria and Weinkamer, Richard and Brumfeld, Vlad and Zheng, Keke and Chen, Junning and Swain, Michael V. and Weiner, Steve and Li, Qing}},
issn = {{1047-8477}},
keywords = {{Fast-Fourier transform; Fibrous network quantification; Loading response; Phase-contrast micro-CT; Soft tissue heterogeneity}},
language = {{eng}},
number = {{3}},
publisher = {{Elsevier}},
series = {{Journal of Structural Biology}},
title = {{Microstructural heterogeneity of the collagenous network in the loaded and unloaded periodontal ligament and its biomechanical implications}},
url = {{http://dx.doi.org/10.1016/j.jsb.2021.107772}},
doi = {{10.1016/j.jsb.2021.107772}},
volume = {{213}},
year = {{2021}},
}