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Lack of arbuscular mycorrhizal colonisation in tea (Camellia sinensis L.) plants cultivated in Northern Iran

Aliasgharzad, Nasser; Hajiboland, Roghieh and Olsson, Pål Axel LU (2011) In Symbiosis 55(2). p.91-95
Abstract
Soil and roots associated with different tea clones and nearby weeds (Veronica sp., Setaria sp., Salvia sp., Senecio sp. and Tripogon sp.) were sampled for arbuscular mycorrhizal fungi (AMF) in the tea gardens of Northern Iran. Spores were searched for in the soil and AMF colonisation determined microscopically and fatty acid signatures in roots was determined. Root samples from mycorrhizal and non-mycorrhizal clover were used as positive and negative controls. AMF spores were abundant in the tea garden soils; the genera Glomus and Acaulospora dominated. Microscopic observations of stained tea roots showed no sign of AMF. To confirm this, the roots were analysed for fatty acid signature compounds. The average level of PLFA 16:1 omega 5 as... (More)
Soil and roots associated with different tea clones and nearby weeds (Veronica sp., Setaria sp., Salvia sp., Senecio sp. and Tripogon sp.) were sampled for arbuscular mycorrhizal fungi (AMF) in the tea gardens of Northern Iran. Spores were searched for in the soil and AMF colonisation determined microscopically and fatty acid signatures in roots was determined. Root samples from mycorrhizal and non-mycorrhizal clover were used as positive and negative controls. AMF spores were abundant in the tea garden soils; the genera Glomus and Acaulospora dominated. Microscopic observations of stained tea roots showed no sign of AMF. To confirm this, the roots were analysed for fatty acid signature compounds. The average level of PLFA 16:1 omega 5 as signature molecule for AMF in tea roots was 2 nmol g(-1) dry root, while the NLFA 16: 1 omega 5 was not detectable. In mycorrhizal and non-mycorrhizal clover roots, the PLFA 16:1 omega 5 was 141 and 5.74 nmol g(-1) dry root, respectively. In roots of weeds in tea plantations, the amount of PLFA 16:1 omega 5 was in the range 4.9 to 31.1 nmol g(-1) dry root. Thus, there was no evidence for AMF association in tea roots and weeds are thought to be the source of the spores in the soils. Finally, no mycorrhizal colonisation was found when tea plant seedlings were inoculated with AMF in pot cultures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
NLFA, PLFA, colonisation, Root, Arbuscular mycorrhizal fungi, Tea, Camellia sinensis L.
in
Symbiosis
volume
55
issue
2
pages
91 - 95
publisher
Balaban Publishers
external identifiers
  • wos:000301279400005
  • scopus:84878216031
ISSN
0334-5114
DOI
10.1007/s13199-011-0149-8
language
English
LU publication?
yes
id
284f4d26-7c47-4521-8d3b-9b7f7f1ed8d5 (old id 2493664)
date added to LUP
2012-05-11 15:41:06
date last changed
2017-03-12 03:47:07
@article{284f4d26-7c47-4521-8d3b-9b7f7f1ed8d5,
  abstract     = {Soil and roots associated with different tea clones and nearby weeds (Veronica sp., Setaria sp., Salvia sp., Senecio sp. and Tripogon sp.) were sampled for arbuscular mycorrhizal fungi (AMF) in the tea gardens of Northern Iran. Spores were searched for in the soil and AMF colonisation determined microscopically and fatty acid signatures in roots was determined. Root samples from mycorrhizal and non-mycorrhizal clover were used as positive and negative controls. AMF spores were abundant in the tea garden soils; the genera Glomus and Acaulospora dominated. Microscopic observations of stained tea roots showed no sign of AMF. To confirm this, the roots were analysed for fatty acid signature compounds. The average level of PLFA 16:1 omega 5 as signature molecule for AMF in tea roots was 2 nmol g(-1) dry root, while the NLFA 16: 1 omega 5 was not detectable. In mycorrhizal and non-mycorrhizal clover roots, the PLFA 16:1 omega 5 was 141 and 5.74 nmol g(-1) dry root, respectively. In roots of weeds in tea plantations, the amount of PLFA 16:1 omega 5 was in the range 4.9 to 31.1 nmol g(-1) dry root. Thus, there was no evidence for AMF association in tea roots and weeds are thought to be the source of the spores in the soils. Finally, no mycorrhizal colonisation was found when tea plant seedlings were inoculated with AMF in pot cultures.},
  author       = {Aliasgharzad, Nasser and Hajiboland, Roghieh and Olsson, Pål Axel},
  issn         = {0334-5114},
  keyword      = {NLFA,PLFA,colonisation,Root,Arbuscular mycorrhizal fungi,Tea,Camellia sinensis L.},
  language     = {eng},
  number       = {2},
  pages        = {91--95},
  publisher    = {Balaban Publishers},
  series       = {Symbiosis},
  title        = {Lack of arbuscular mycorrhizal colonisation in tea (Camellia sinensis L.) plants cultivated in Northern Iran},
  url          = {http://dx.doi.org/10.1007/s13199-011-0149-8},
  volume       = {55},
  year         = {2011},
}