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Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores

Bozhanova, Nina G.; Baranov, Mikhail S.; Sarkisyan, Karen S.; Gritcenko, Roman LU ; Mineev, Konstantin S.; Golodukhina, Svetlana V.; Baleeva, Nadezhda S.; Lukyanov, Konstantin A. and Mishin, Alexander S. (2017) In ACS Chemical Biology 12(7). p.1867-1873
Abstract

Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Chemical Biology
volume
12
issue
7
pages
7 pages
publisher
The American Chemical Society
external identifiers
  • scopus:85025170752
  • wos:000406356300019
ISSN
1554-8929
DOI
10.1021/acschembio.7b00337
language
English
LU publication?
yes
id
24bf9cae-259d-4593-992b-db2b08b7efd8
date added to LUP
2017-07-31 09:50:57
date last changed
2017-09-18 11:40:21
@article{24bf9cae-259d-4593-992b-db2b08b7efd8,
  abstract     = {<p>Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.</p>},
  author       = {Bozhanova, Nina G. and Baranov, Mikhail S. and Sarkisyan, Karen S. and Gritcenko, Roman and Mineev, Konstantin S. and Golodukhina, Svetlana V. and Baleeva, Nadezhda S. and Lukyanov, Konstantin A. and Mishin, Alexander S.},
  issn         = {1554-8929},
  language     = {eng},
  month        = {07},
  number       = {7},
  pages        = {1867--1873},
  publisher    = {The American Chemical Society},
  series       = {ACS Chemical Biology},
  title        = {Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores},
  url          = {http://dx.doi.org/10.1021/acschembio.7b00337},
  volume       = {12},
  year         = {2017},
}