Production of the artemisinin precursor amorpha-4,11-diene by engineered Saccharomyces cerevisiae
(2006) In Biotechnology Letters 28(8). p.80-571- Abstract
The gene encoding for amorpha-4,11-diene synthase from Artemisia annua was transformed into yeast Saccharomyces cerevisiae in two fundamentally different ways. First, the gene was subcloned into the galactose-inducible, high-copy number yeast expression vector pYeDP60 and used to transform the Saccharomyces cerevisiae strain CEN.PK113-5D. Secondly, amorpha-4,11-diene synthase gene, regulated by the same promoter, was introduced into the yeast genome by homologous recombination. In protein extracts from galactose-induced yeast cells, a higher activity was observed for yeast expressing the enzyme from the plasmid. The genome-transformed yeast grows at the same rate as wild-type yeast while plasmid-carrying yeast grows somewhat slower than... (More)
The gene encoding for amorpha-4,11-diene synthase from Artemisia annua was transformed into yeast Saccharomyces cerevisiae in two fundamentally different ways. First, the gene was subcloned into the galactose-inducible, high-copy number yeast expression vector pYeDP60 and used to transform the Saccharomyces cerevisiae strain CEN.PK113-5D. Secondly, amorpha-4,11-diene synthase gene, regulated by the same promoter, was introduced into the yeast genome by homologous recombination. In protein extracts from galactose-induced yeast cells, a higher activity was observed for yeast expressing the enzyme from the plasmid. The genome-transformed yeast grows at the same rate as wild-type yeast while plasmid-carrying yeast grows somewhat slower than the wild-type yeast. The plasmid and genome-transformed yeasts produced 600 and 100 microg/l of the artemisinin precursor amorpha-4,11-diene, respectively, during 16-days' batch cultivation.
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- author
- Lindahl, Ann-Louise ; Olsson, Mikael E ; Mercke, Per LU ; Tollbom, Orjan ; Schelin, Jenny LU ; Brodelius, Maria and Brodelius, Peter E
- publishing date
- 2006-04
- type
- Contribution to journal
- publication status
- published
- keywords
- Alkyl and Aryl Transferases/genetics, Artemisia annua/enzymology, Artemisinins/chemistry, Blotting, Western, Gas Chromatography-Mass Spectrometry, Genetic Engineering/methods, Genome, Fungal/genetics, Molecular Structure, Plasmids/genetics, Polyisoprenyl Phosphates/chemistry, Recombination, Genetic, Saccharomyces cerevisiae/genetics, Sesquiterpenes/chemistry, Transformation, Genetic
- in
- Biotechnology Letters
- volume
- 28
- issue
- 8
- pages
- 10 pages
- publisher
- Springer
- external identifiers
-
- scopus:33645851015
- pmid:16614895
- ISSN
- 0141-5492
- DOI
- 10.1007/s10529-006-0015-6
- language
- English
- LU publication?
- no
- id
- 250aef2d-742d-45a7-a643-b031800f9a59
- date added to LUP
- 2018-06-11 11:57:14
- date last changed
- 2024-03-01 20:33:55
@article{250aef2d-742d-45a7-a643-b031800f9a59,
abstract = {{<p>The gene encoding for amorpha-4,11-diene synthase from Artemisia annua was transformed into yeast Saccharomyces cerevisiae in two fundamentally different ways. First, the gene was subcloned into the galactose-inducible, high-copy number yeast expression vector pYeDP60 and used to transform the Saccharomyces cerevisiae strain CEN.PK113-5D. Secondly, amorpha-4,11-diene synthase gene, regulated by the same promoter, was introduced into the yeast genome by homologous recombination. In protein extracts from galactose-induced yeast cells, a higher activity was observed for yeast expressing the enzyme from the plasmid. The genome-transformed yeast grows at the same rate as wild-type yeast while plasmid-carrying yeast grows somewhat slower than the wild-type yeast. The plasmid and genome-transformed yeasts produced 600 and 100 microg/l of the artemisinin precursor amorpha-4,11-diene, respectively, during 16-days' batch cultivation.</p>}},
author = {{Lindahl, Ann-Louise and Olsson, Mikael E and Mercke, Per and Tollbom, Orjan and Schelin, Jenny and Brodelius, Maria and Brodelius, Peter E}},
issn = {{0141-5492}},
keywords = {{Alkyl and Aryl Transferases/genetics; Artemisia annua/enzymology; Artemisinins/chemistry; Blotting, Western; Gas Chromatography-Mass Spectrometry; Genetic Engineering/methods; Genome, Fungal/genetics; Molecular Structure; Plasmids/genetics; Polyisoprenyl Phosphates/chemistry; Recombination, Genetic; Saccharomyces cerevisiae/genetics; Sesquiterpenes/chemistry; Transformation, Genetic}},
language = {{eng}},
number = {{8}},
pages = {{80--571}},
publisher = {{Springer}},
series = {{Biotechnology Letters}},
title = {{Production of the artemisinin precursor amorpha-4,11-diene by engineered Saccharomyces cerevisiae}},
url = {{http://dx.doi.org/10.1007/s10529-006-0015-6}},
doi = {{10.1007/s10529-006-0015-6}},
volume = {{28}},
year = {{2006}},
}