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Versatile automated continuous flow system (VersAFlo) for bioanalysis and bioprocess control

Kumar, M A LU ; Mazlomi, Mohammad LU ; Hedström, Martin LU and Mattiasson, Bo LU (2012) In Sensors and Actuators B: Chemical 161(1). p.855-861
Abstract
Flow injection analysis provides a convenient platform for the use of biosensors in the monitoring of bio-processes. A major requirement in bioprocess monitoring is real time information about various process parameters like concentration of products and substrates, flow rates, and injection volumes. Depending on the application, the monitoring requirements vary and this results in a need for different measurement flow schemes. Common flow schemes employed with biosensing are FIA, SIA, BIA and multi-commuted flow. In this work, a versatile platform to employ biosensors for continuous analysis of bioprocesses with precise control of flow, volume and defined events has been developed. The system is based on National Instruments LabVIEW and... (More)
Flow injection analysis provides a convenient platform for the use of biosensors in the monitoring of bio-processes. A major requirement in bioprocess monitoring is real time information about various process parameters like concentration of products and substrates, flow rates, and injection volumes. Depending on the application, the monitoring requirements vary and this results in a need for different measurement flow schemes. Common flow schemes employed with biosensing are FIA, SIA, BIA and multi-commuted flow. In this work, a versatile platform to employ biosensors for continuous analysis of bioprocesses with precise control of flow, volume and defined events has been developed. The system is based on National Instruments LabVIEW and employs piston-, peristaltic pumps, motorized injection valves and 2-way and 3-way solenoid valves. The system supports data acquisition, controls interface with devices and visualization of data from multiple detectors used in biosensing. The system was successfully evaluated for the analysis of a model analyte, human immunoglobulin G (IgG), employing continuous flow-ELISA in competitive mode with optical detection. The flow-immunosensor showed very good linearity; from 5 to 400 mu g/ml. The system had excellent reproducibility and stability with a relative standard deviation of 4.75%. The immunosensor was also subjected to a model first order kinetics of target analyte concentration and the results from the sensor agreed well with the estimated concentration. The flexibility of the system gives that different flow configurations easily can be set up and the dynamic range of analysis can be adapted by varying different flow conditions for measurement, thereby controlling different reaction times in the biosensor system. The system could be useful for online analysis and control of bioprocesses employing biosensors. Further, with automated data collection and analysis, the system provides excellent basis for cross platform data communication for integrated control. (C) 2011 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IgG-analysis, SIA, FIA, Bioanalysis, Bioprocess, Automation, Flow-ELISA, Biosensors, Online monitoring, Immunoassay
in
Sensors and Actuators B: Chemical
volume
161
issue
1
pages
855 - 861
publisher
Elsevier
external identifiers
  • wos:000301549400116
  • scopus:84856229688
ISSN
0925-4005
DOI
10.1016/j.snb.2011.11.049
language
English
LU publication?
yes
id
d2857acb-31f8-42bf-901f-1d00d7cd85ac (old id 2515271)
date added to LUP
2012-05-15 08:31:17
date last changed
2017-07-30 04:08:51
@article{d2857acb-31f8-42bf-901f-1d00d7cd85ac,
  abstract     = {Flow injection analysis provides a convenient platform for the use of biosensors in the monitoring of bio-processes. A major requirement in bioprocess monitoring is real time information about various process parameters like concentration of products and substrates, flow rates, and injection volumes. Depending on the application, the monitoring requirements vary and this results in a need for different measurement flow schemes. Common flow schemes employed with biosensing are FIA, SIA, BIA and multi-commuted flow. In this work, a versatile platform to employ biosensors for continuous analysis of bioprocesses with precise control of flow, volume and defined events has been developed. The system is based on National Instruments LabVIEW and employs piston-, peristaltic pumps, motorized injection valves and 2-way and 3-way solenoid valves. The system supports data acquisition, controls interface with devices and visualization of data from multiple detectors used in biosensing. The system was successfully evaluated for the analysis of a model analyte, human immunoglobulin G (IgG), employing continuous flow-ELISA in competitive mode with optical detection. The flow-immunosensor showed very good linearity; from 5 to 400 mu g/ml. The system had excellent reproducibility and stability with a relative standard deviation of 4.75%. The immunosensor was also subjected to a model first order kinetics of target analyte concentration and the results from the sensor agreed well with the estimated concentration. The flexibility of the system gives that different flow configurations easily can be set up and the dynamic range of analysis can be adapted by varying different flow conditions for measurement, thereby controlling different reaction times in the biosensor system. The system could be useful for online analysis and control of bioprocesses employing biosensors. Further, with automated data collection and analysis, the system provides excellent basis for cross platform data communication for integrated control. (C) 2011 Elsevier B.V. All rights reserved.},
  author       = {Kumar, M A and Mazlomi, Mohammad and Hedström, Martin and Mattiasson, Bo},
  issn         = {0925-4005},
  keyword      = {IgG-analysis,SIA,FIA,Bioanalysis,Bioprocess,Automation,Flow-ELISA,Biosensors,Online monitoring,Immunoassay},
  language     = {eng},
  number       = {1},
  pages        = {855--861},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Versatile automated continuous flow system (VersAFlo) for bioanalysis and bioprocess control},
  url          = {http://dx.doi.org/10.1016/j.snb.2011.11.049},
  volume       = {161},
  year         = {2012},
}