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Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog

Rehfeld, Jens F. and Ericsson, Peter LU (2012) In Scandinavian Journal of Clinical & Laboratory Investigation 72(2). p.175-179
Abstract
Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using... (More)
Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
peptide hormones, radioimmunoassay (RIA), homology, cholecystokinin (CCK), gastrin, Antibody cooperativity
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
72
issue
2
pages
175 - 179
publisher
Informa Healthcare
external identifiers
  • wos:000301456100015
  • scopus:84858423079
ISSN
1502-7686
DOI
10.3109/00365513.2011.637130
language
English
LU publication?
yes
id
00df49a6-89be-4c52-974e-100235ec7e03 (old id 2515386)
date added to LUP
2012-05-07 08:48:18
date last changed
2017-01-01 05:36:48
@article{00df49a6-89be-4c52-974e-100235ec7e03,
  abstract     = {Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.},
  author       = {Rehfeld, Jens F. and Ericsson, Peter},
  issn         = {1502-7686},
  keyword      = {peptide hormones,radioimmunoassay (RIA),homology,cholecystokinin (CCK),gastrin,Antibody cooperativity},
  language     = {eng},
  number       = {2},
  pages        = {175--179},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog},
  url          = {http://dx.doi.org/10.3109/00365513.2011.637130},
  volume       = {72},
  year         = {2012},
}