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Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury

Kutlu, O.; Ross, A. E.; Schaeffer, E. M.; Gratzke, C.; Stief, C. G.; Strong, T. D.; Burnett, A. L.; Hedlund, Petter LU and Bivalacqua, T. J. (2012) In International Journal of Impotence Research 24(2). p.84-90
Abstract
In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery... (More)
In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury. International Journal of Impotence Research (2012) 24, 84-90; doi:10.1038/ijir.2011.50; published online 13 October 2011 (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
stem cell, radical prostatectomy, prostate cancer, erectile dysfunction
in
International Journal of Impotence Research
volume
24
issue
2
pages
84 - 90
publisher
Nature Publishing Group
external identifiers
  • wos:000301714200005
  • scopus:84858704689
ISSN
1476-5489
DOI
10.1038/ijir.2011.50
language
English
LU publication?
yes
id
df406c91-1d09-4c00-af4a-1f3402298912 (old id 2515729)
date added to LUP
2012-05-07 08:13:23
date last changed
2017-01-08 03:10:56
@article{df406c91-1d09-4c00-af4a-1f3402298912,
  abstract     = {In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P&lt;0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P&lt;0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury. International Journal of Impotence Research (2012) 24, 84-90; doi:10.1038/ijir.2011.50; published online 13 October 2011},
  author       = {Kutlu, O. and Ross, A. E. and Schaeffer, E. M. and Gratzke, C. and Stief, C. G. and Strong, T. D. and Burnett, A. L. and Hedlund, Petter and Bivalacqua, T. J.},
  issn         = {1476-5489},
  keyword      = {stem cell,radical prostatectomy,prostate cancer,erectile dysfunction},
  language     = {eng},
  number       = {2},
  pages        = {84--90},
  publisher    = {Nature Publishing Group},
  series       = {International Journal of Impotence Research},
  title        = {Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury},
  url          = {http://dx.doi.org/10.1038/ijir.2011.50},
  volume       = {24},
  year         = {2012},
}