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Extraction of polar lipids from bifidobacteria by supercritical carbon dioxide (scCO(2))

Izhyk, Anastasiya; Novik, Galina and Dey, Estera LU (2012) In Journal of Supercritical Fluids 62. p.149-154
Abstract
This study describes an efficient isolation procedure for the extraction of polar lipids from two strains of bifidobacteria - Bifidobacterium longum and Bifidobacterium angulatum. We first compared the lipid profiles of two strains of bifidobacteria and showed that the strains of the same genus may differ by lipid composition of cell walls. The polar lipids were isolated using the following conditions: pressure 250 bar, temperature 45 degrees C, CO2 flow rate 5 g/min, hydromethanolic co-solvent concentration 10% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids phosphorus moiety were quantified and compared to the reference material isolated by classical extraction. Thrice the amount of glycolipids... (More)
This study describes an efficient isolation procedure for the extraction of polar lipids from two strains of bifidobacteria - Bifidobacterium longum and Bifidobacterium angulatum. We first compared the lipid profiles of two strains of bifidobacteria and showed that the strains of the same genus may differ by lipid composition of cell walls. The polar lipids were isolated using the following conditions: pressure 250 bar, temperature 45 degrees C, CO2 flow rate 5 g/min, hydromethanolic co-solvent concentration 10% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids phosphorus moiety were quantified and compared to the reference material isolated by classical extraction. Thrice the amount of glycolipids and phospholipids in comparison with classical method was found in the lipid extract from B. longum obtained with scCO(2), and twice the amount of more - from B. angulatum. By TLC were identified different glycolipid profiles of these strains, four glycolipids were detected from B. longum and seven glycolipids from B. angulatum. From 5 g of biomass during prolonged fractionated extraction (12 h) 2.80 and 3.11 mg of glycolipids, 1.86 and 1.88 mg of phospholipids from B. longum and B. angulatum was obtained, respectively. (C) 2011 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phospholipids, Glycolipids, angulatum, Bifidobacterium, Supercritical carbon dioxide, Bifidobaterium longum
in
Journal of Supercritical Fluids
volume
62
pages
149 - 154
publisher
Elsevier
external identifiers
  • wos:000301327100017
  • scopus:84855849124
ISSN
0896-8446
DOI
10.1016/j.supflu.2011.10.013
language
English
LU publication?
yes
id
52a2b42d-96a8-420d-9ba5-d0dc68b9446c (old id 2515771)
date added to LUP
2012-05-09 14:21:35
date last changed
2017-01-01 03:30:32
@article{52a2b42d-96a8-420d-9ba5-d0dc68b9446c,
  abstract     = {This study describes an efficient isolation procedure for the extraction of polar lipids from two strains of bifidobacteria - Bifidobacterium longum and Bifidobacterium angulatum. We first compared the lipid profiles of two strains of bifidobacteria and showed that the strains of the same genus may differ by lipid composition of cell walls. The polar lipids were isolated using the following conditions: pressure 250 bar, temperature 45 degrees C, CO2 flow rate 5 g/min, hydromethanolic co-solvent concentration 10% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids phosphorus moiety were quantified and compared to the reference material isolated by classical extraction. Thrice the amount of glycolipids and phospholipids in comparison with classical method was found in the lipid extract from B. longum obtained with scCO(2), and twice the amount of more - from B. angulatum. By TLC were identified different glycolipid profiles of these strains, four glycolipids were detected from B. longum and seven glycolipids from B. angulatum. From 5 g of biomass during prolonged fractionated extraction (12 h) 2.80 and 3.11 mg of glycolipids, 1.86 and 1.88 mg of phospholipids from B. longum and B. angulatum was obtained, respectively. (C) 2011 Elsevier B.V. All rights reserved.},
  author       = {Izhyk, Anastasiya and Novik, Galina and Dey, Estera},
  issn         = {0896-8446},
  keyword      = {Phospholipids,Glycolipids,angulatum,Bifidobacterium,Supercritical carbon dioxide,Bifidobaterium longum},
  language     = {eng},
  pages        = {149--154},
  publisher    = {Elsevier},
  series       = {Journal of Supercritical Fluids},
  title        = {Extraction of polar lipids from bifidobacteria by supercritical carbon dioxide (scCO(2))},
  url          = {http://dx.doi.org/10.1016/j.supflu.2011.10.013},
  volume       = {62},
  year         = {2012},
}