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Low salivary IgA activity to cell-surface antigens of mutans streptococci related to HLA-DRB1*04

Wallengren, MLL; Hamberg, K; Ericson, D and Nordberg, Jonas LU (2005) In Oral Microbiology and Immunology 20(2). p.73-81
Abstract
Background/aims: Mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. These differences are not readily explained, though several factors are believed to influence the colonization. One factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous study, differences in salivary IgA reactions to oral streptococci were observed between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. A lower salivary IgA activity to Streptococcus mutans in particular was most pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this study was to further investigate, in a... (More)
Background/aims: Mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. These differences are not readily explained, though several factors are believed to influence the colonization. One factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous study, differences in salivary IgA reactions to oral streptococci were observed between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. A lower salivary IgA activity to Streptococcus mutans in particular was most pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this study was to further investigate, in a larger study group, the salivary IgA activity to antigens of three oral streptococci in relation to different HLA-DRB1*04 alleles. Methods: Stimulated saliva was collected from 58 HLA-DRB1*04-positive individuals. Whole cell antigen extracts from S. mutans, Streptococcus sobrinus and Streptococcus parasanguis and the streptococcal antigen (SA) I/II were separated in SDS-PAGE, transblotted and detected with diluted saliva (Western blot), and analyzed in a computer program. All distinct immunoblot bands over 100 kDa were recorded and compared in relation to DRB1*04. Results: The immunoblots revealed lower salivary IgA reactions to S. mutans, S. sobrinus and SA I/II, but not to S. parasanguis, for the DRB1*0401- and *0404-positive individuals compared to other DRB1*04 types. For the *0401 subgroup there was a significant association with a lower IgA response to S. mutans. Conclusion: The results confirm earlier observations and may also support previous demonstrated association between colonization by mutans streptococci and the serologically defined HLA-DR4. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
salivary IgA, oral streptococci, cell-surface antigen, HLA, Western blot
in
Oral Microbiology and Immunology
volume
20
issue
2
pages
73 - 81
publisher
Wiley-Blackwell
external identifiers
  • pmid:15720566
  • wos:000227130300002
  • scopus:14944342448
ISSN
0902-0055
DOI
10.1111/j.1399-302X.2004.00192.x
language
English
LU publication?
yes
id
48952bb5-4d34-4684-b45b-6162db65fddf (old id 253464)
date added to LUP
2007-08-16 17:04:13
date last changed
2017-02-26 04:13:35
@article{48952bb5-4d34-4684-b45b-6162db65fddf,
  abstract     = {Background/aims: Mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. These differences are not readily explained, though several factors are believed to influence the colonization. One factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous study, differences in salivary IgA reactions to oral streptococci were observed between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. A lower salivary IgA activity to Streptococcus mutans in particular was most pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this study was to further investigate, in a larger study group, the salivary IgA activity to antigens of three oral streptococci in relation to different HLA-DRB1*04 alleles. Methods: Stimulated saliva was collected from 58 HLA-DRB1*04-positive individuals. Whole cell antigen extracts from S. mutans, Streptococcus sobrinus and Streptococcus parasanguis and the streptococcal antigen (SA) I/II were separated in SDS-PAGE, transblotted and detected with diluted saliva (Western blot), and analyzed in a computer program. All distinct immunoblot bands over 100 kDa were recorded and compared in relation to DRB1*04. Results: The immunoblots revealed lower salivary IgA reactions to S. mutans, S. sobrinus and SA I/II, but not to S. parasanguis, for the DRB1*0401- and *0404-positive individuals compared to other DRB1*04 types. For the *0401 subgroup there was a significant association with a lower IgA response to S. mutans. Conclusion: The results confirm earlier observations and may also support previous demonstrated association between colonization by mutans streptococci and the serologically defined HLA-DR4.},
  author       = {Wallengren, MLL and Hamberg, K and Ericson, D and Nordberg, Jonas},
  issn         = {0902-0055},
  keyword      = {salivary IgA,oral streptococci,cell-surface antigen,HLA,Western blot},
  language     = {eng},
  number       = {2},
  pages        = {73--81},
  publisher    = {Wiley-Blackwell},
  series       = {Oral Microbiology and Immunology},
  title        = {Low salivary IgA activity to cell-surface antigens of mutans streptococci related to HLA-DRB1*04},
  url          = {http://dx.doi.org/10.1111/j.1399-302X.2004.00192.x},
  volume       = {20},
  year         = {2005},
}