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Functional analysis of the classical, alternative, and MBL pathways of the complement system: standardization and validation of a simple ELISA

Seelen, MA; Roos, A; Wieslander, J; Mollnes, TE; Sjöholm, Anders LU ; Wurzner, R; Loos, M; Tedesco, F; Sim, RB and Garred, P, et al. (2005) In Journal of Immunological Methods 296(1-2). p.187-198
Abstract
Primary defence against invading microorganisms depends on a functional innate immune system and the complement system plays a major role in such immunity. Deficiencies in one of the components of the complement system can cause severe and recurrent infections, systemic diseases, such as systemic lupus erythematosus (SLE) and renal disease. Screening for complement deficiencies in the classical or alternative complement pathways has mainly been performed by haemolytic assays. Here. we describe a simple ELISA-based format for the evaluation of three pathways of complement activation. The assays are based on specific coatings for each pathway in combination with specific buffer systems. We have standardized these assays and defined cut off... (More)
Primary defence against invading microorganisms depends on a functional innate immune system and the complement system plays a major role in such immunity. Deficiencies in one of the components of the complement system can cause severe and recurrent infections, systemic diseases, such as systemic lupus erythematosus (SLE) and renal disease. Screening for complement deficiencies in the classical or alternative complement pathways has mainly been performed by haemolytic assays. Here. we describe a simple ELISA-based format for the evaluation of three pathways of complement activation. The assays are based on specific coatings for each pathway in combination with specific buffer systems. We have standardized these assays and defined cut off values to detect complement deficiencies at the different levels of the complement system. The results demonstrate the value of these ELISA-based procedures for the functional assessment of complement deficiencies in clinical practice. The assay is now available commercially in kit form. (Less)
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type
Contribution to journal
publication status
published
subject
keywords
systemic lupus erythematosus, MBL pathways, ELISA
in
Journal of Immunological Methods
volume
296
issue
1-2
pages
187 - 198
publisher
Elsevier
external identifiers
  • wos:000227115700019
  • pmid:15680163
  • scopus:19944430192
ISSN
1872-7905
DOI
10.1016/j.jim.2004.11.016
language
English
LU publication?
yes
id
956ec6ed-392c-4b96-914e-bd2d6f93cdf9 (old id 253576)
date added to LUP
2007-08-13 16:04:29
date last changed
2017-10-01 04:54:43
@article{956ec6ed-392c-4b96-914e-bd2d6f93cdf9,
  abstract     = {Primary defence against invading microorganisms depends on a functional innate immune system and the complement system plays a major role in such immunity. Deficiencies in one of the components of the complement system can cause severe and recurrent infections, systemic diseases, such as systemic lupus erythematosus (SLE) and renal disease. Screening for complement deficiencies in the classical or alternative complement pathways has mainly been performed by haemolytic assays. Here. we describe a simple ELISA-based format for the evaluation of three pathways of complement activation. The assays are based on specific coatings for each pathway in combination with specific buffer systems. We have standardized these assays and defined cut off values to detect complement deficiencies at the different levels of the complement system. The results demonstrate the value of these ELISA-based procedures for the functional assessment of complement deficiencies in clinical practice. The assay is now available commercially in kit form.},
  author       = {Seelen, MA and Roos, A and Wieslander, J and Mollnes, TE and Sjöholm, Anders and Wurzner, R and Loos, M and Tedesco, F and Sim, RB and Garred, P and Alexopoulos, E and Turner, MW and Daha, MR},
  issn         = {1872-7905},
  keyword      = {systemic lupus erythematosus,MBL pathways,ELISA},
  language     = {eng},
  number       = {1-2},
  pages        = {187--198},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Functional analysis of the classical, alternative, and MBL pathways of the complement system: standardization and validation of a simple ELISA},
  url          = {http://dx.doi.org/10.1016/j.jim.2004.11.016},
  volume       = {296},
  year         = {2005},
}