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Generation and molecular characterization of head and neck squamous cell lines of Fanconi anemia patients

van Zeeburg, HJT; Snijders, PJF; Pals, G; Hermsen, MAA; Rooimans, MA; Bagby, G; Soulier, J; Gluckman, E; Wennerberg, Johan LU and Leemans, CR, et al. (2005) In Cancer Research 65(4). p.1271-1276
Abstract
Patients with Fanconi anemia (FA) are prone to develop malignancies at an early age. Besides hematologic malignancies, squamous cell carcinomas in the anogenital region and head and neck are also frequently found in these patients. The aim of this study was to generate a panel of head and neck squamous cell carcinoma (HNSCC) cell lines and xenografts of FA HNSCC, and to characterize these cell lines in comparison with a panel of seven cell lines from patients with sporadic HNSCC. Analyses have been done on sensitivity to DNA cross-linking agents, loss of heterozygosity profile, TP53 mutations, TP53 polymorph isms and the presence of human papillomavirus. Four FA HNSCC cell lines were established. Sensitivity to DTNA cross-linking agents... (More)
Patients with Fanconi anemia (FA) are prone to develop malignancies at an early age. Besides hematologic malignancies, squamous cell carcinomas in the anogenital region and head and neck are also frequently found in these patients. The aim of this study was to generate a panel of head and neck squamous cell carcinoma (HNSCC) cell lines and xenografts of FA HNSCC, and to characterize these cell lines in comparison with a panel of seven cell lines from patients with sporadic HNSCC. Analyses have been done on sensitivity to DNA cross-linking agents, loss of heterozygosity profile, TP53 mutations, TP53 polymorph isms and the presence of human papillomavirus. Four FA HNSCC cell lines were established. Sensitivity to DTNA cross-linking agents (cisplatin) in the FA HNSCC cell lines was on average 10 times higher as compared with the sporadic HNSCC cell lines. Human papillomavirus was not detected in any of the FA or sporadic cell lines. No differences were found in loss of heterozygosity pattern, TP53 mutation frequency and TP53 polymorphism between FA and sporadic HNSCC cell lines. This is the first report on the generation of squamous cell lines of FA patients. The FA HNSCC cell lines we have,generated may be utilized for future studies and might aid in the development of new preventive therapies for FA patients. The genetic characteristics of these cell lines suggest that FA HNSCC are not.,cry different from sporadic HNSCC, except for the sensitivity to cisplatin which is consistent with the known cellular FA phenotype. (Less)
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Contribution to journal
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Cancer Research
volume
65
issue
4
pages
1271 - 1276
publisher
American Association for Cancer Research Inc.
external identifiers
  • wos:000226997800021
  • pmid:15735012
  • scopus:13944254363
ISSN
1538-7445
language
English
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yes
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43e1df36-424e-47c7-9f53-4f383460b547 (old id 253829)
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http://cancerres.aacrjournals.org/cgi/content/abstract/65/4/1271
date added to LUP
2007-08-14 11:14:49
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2017-09-17 07:06:45
@article{43e1df36-424e-47c7-9f53-4f383460b547,
  abstract     = {Patients with Fanconi anemia (FA) are prone to develop malignancies at an early age. Besides hematologic malignancies, squamous cell carcinomas in the anogenital region and head and neck are also frequently found in these patients. The aim of this study was to generate a panel of head and neck squamous cell carcinoma (HNSCC) cell lines and xenografts of FA HNSCC, and to characterize these cell lines in comparison with a panel of seven cell lines from patients with sporadic HNSCC. Analyses have been done on sensitivity to DNA cross-linking agents, loss of heterozygosity profile, TP53 mutations, TP53 polymorph isms and the presence of human papillomavirus. Four FA HNSCC cell lines were established. Sensitivity to DTNA cross-linking agents (cisplatin) in the FA HNSCC cell lines was on average 10 times higher as compared with the sporadic HNSCC cell lines. Human papillomavirus was not detected in any of the FA or sporadic cell lines. No differences were found in loss of heterozygosity pattern, TP53 mutation frequency and TP53 polymorphism between FA and sporadic HNSCC cell lines. This is the first report on the generation of squamous cell lines of FA patients. The FA HNSCC cell lines we have,generated may be utilized for future studies and might aid in the development of new preventive therapies for FA patients. The genetic characteristics of these cell lines suggest that FA HNSCC are not.,cry different from sporadic HNSCC, except for the sensitivity to cisplatin which is consistent with the known cellular FA phenotype.},
  author       = {van Zeeburg, HJT and Snijders, PJF and Pals, G and Hermsen, MAA and Rooimans, MA and Bagby, G and Soulier, J and Gluckman, E and Wennerberg, Johan and Leemans, CR and Joenje, H and Brakenhoff, RH},
  issn         = {1538-7445},
  language     = {eng},
  number       = {4},
  pages        = {1271--1276},
  publisher    = {American Association for Cancer Research Inc.},
  series       = {Cancer Research},
  title        = {Generation and molecular characterization of head and neck squamous cell lines of Fanconi anemia patients},
  volume       = {65},
  year         = {2005},
}