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Simultaneous detection of the release of glutamate and nitric oxide from adherently growing cells using an array of glutamate and nitric oxide selective electrodes

Castillo Leon, Jaime LU ; Isik, S; Blochl, A; Pereira-Rodrigues, N; Bedioui, F; Csöregi, Elisabeth LU ; Schuhmann, W and Oni, J (2005) In Biosensors & Bioelectronics 20(8). p.1559-1565
Abstract
The simultaneous detection of nitric oxide and glutamate using an array of individually addressable electrodes, in which the individual electrodes in the array were suitably modified with a highly sensitive nitric oxide sensing chemistry or a glutamate oxidase/redox hydrogel-based glutamate biosensor is presented. In a sequence of modification steps one of the electrodes was covered first with a positively charged Ni porphyrin entrapped into a negatively charged electrodeposition paint followed by the manual modification of the second working electrode by a bienzyme sensor architecture based on crosslinked redox hydrogels with entrapped peroxidase and glutamate oxidase. Adherently growing C6-glioma cells were grown on membrane inserts and... (More)
The simultaneous detection of nitric oxide and glutamate using an array of individually addressable electrodes, in which the individual electrodes in the array were suitably modified with a highly sensitive nitric oxide sensing chemistry or a glutamate oxidase/redox hydrogel-based glutamate biosensor is presented. In a sequence of modification steps one of the electrodes was covered first with a positively charged Ni porphyrin entrapped into a negatively charged electrodeposition paint followed by the manual modification of the second working electrode by a bienzyme sensor architecture based on crosslinked redox hydrogels with entrapped peroxidase and glutamate oxidase. Adherently growing C6-glioma cells were grown on membrane inserts and placed in close distance to the modified sensor surfaces. The current responses recorded at each electrode after stimulation of glutamate and NO release by means of K+ and bradykinin clearly demonstrate the ability of the individual electrode in the array to detect the analyte towards which its sensitivity and selectivity was targeted without interference from the neighbouring electrode or other analytes present in the test mixture. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nitric oxide, glutamate oxidase, electrode array, biosensor, modified, electrodes, transmitter release
in
Biosensors & Bioelectronics
volume
20
issue
8
pages
1559 - 1565
publisher
Elsevier
external identifiers
  • wos:000227022700012
  • pmid:15626609
  • scopus:11144236559
ISSN
1873-4235
DOI
10.1016/j.bios.2004.08.021
language
English
LU publication?
yes
id
1e522598-ee4a-4777-b466-d0e605f6e4fc (old id 253867)
date added to LUP
2007-08-24 13:00:10
date last changed
2017-01-29 04:01:46
@article{1e522598-ee4a-4777-b466-d0e605f6e4fc,
  abstract     = {The simultaneous detection of nitric oxide and glutamate using an array of individually addressable electrodes, in which the individual electrodes in the array were suitably modified with a highly sensitive nitric oxide sensing chemistry or a glutamate oxidase/redox hydrogel-based glutamate biosensor is presented. In a sequence of modification steps one of the electrodes was covered first with a positively charged Ni porphyrin entrapped into a negatively charged electrodeposition paint followed by the manual modification of the second working electrode by a bienzyme sensor architecture based on crosslinked redox hydrogels with entrapped peroxidase and glutamate oxidase. Adherently growing C6-glioma cells were grown on membrane inserts and placed in close distance to the modified sensor surfaces. The current responses recorded at each electrode after stimulation of glutamate and NO release by means of K+ and bradykinin clearly demonstrate the ability of the individual electrode in the array to detect the analyte towards which its sensitivity and selectivity was targeted without interference from the neighbouring electrode or other analytes present in the test mixture.},
  author       = {Castillo Leon, Jaime and Isik, S and Blochl, A and Pereira-Rodrigues, N and Bedioui, F and Csöregi, Elisabeth and Schuhmann, W and Oni, J},
  issn         = {1873-4235},
  keyword      = {nitric oxide,glutamate oxidase,electrode array,biosensor,modified,electrodes,transmitter release},
  language     = {eng},
  number       = {8},
  pages        = {1559--1565},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Simultaneous detection of the release of glutamate and nitric oxide from adherently growing cells using an array of glutamate and nitric oxide selective electrodes},
  url          = {http://dx.doi.org/10.1016/j.bios.2004.08.021},
  volume       = {20},
  year         = {2005},
}