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Prospective study of relationship between cytomegalovirus pneumonia and viral load in renal transplant recipients

Ye, Q; Luo, G; He, X; Zheng, W; Zheng, L; Dong, X; Xu, X; Nilsson-Ehle, Peter LU and Xu, Ning LU (2004) In Transplantation Proceedings 36(10). p.3036-3041
Abstract
The present study prospectively examined the relationship between cytomegalovirus interstitial pneumonia (CMV-IP) and viral load among 56 renal transplant recipients. We sought to identify the cutoff of viral load to predict CMV-IP. Blood samples were obtained weekly within the first 2 months and every second week during 2 to 6 months after kidney transplantations. A commercial real-time polymerase chain reaction (PCR)-method was applied to quantify CMV-DNA in plasma or in leukocytes. Among 54 renal transplant recipients who were analyzed for CMV-DNA in the blood (96.4%), 8 experienced CMV-IP (14.3%) and 2 died (3.6%). After kidney transplantation, CMV-DNA loads were near 0 in plasma before the week 4 and before the week 3 in leukocytes... (More)
The present study prospectively examined the relationship between cytomegalovirus interstitial pneumonia (CMV-IP) and viral load among 56 renal transplant recipients. We sought to identify the cutoff of viral load to predict CMV-IP. Blood samples were obtained weekly within the first 2 months and every second week during 2 to 6 months after kidney transplantations. A commercial real-time polymerase chain reaction (PCR)-method was applied to quantify CMV-DNA in plasma or in leukocytes. Among 54 renal transplant recipients who were analyzed for CMV-DNA in the blood (96.4%), 8 experienced CMV-IP (14.3%) and 2 died (3.6%). After kidney transplantation, CMV-DNA loads were near 0 in plasma before the week 4 and before the week 3 in leukocytes among both groups. From week 5 (week 4, in leukocytes), plasma CMV-DNA loads in the CMV-IP group increased, the peak value reached at week 8 in plasma and the week 9 in leukocytes. Whereas, the CMV-DNA loads both in plasma and in leukocytes in the non-CMV-IP group fluctuated at lower levels, those in plasma were significantly different between the 2 groups at the weeks 5, 7, and 9. For CMV-DNA in leukocytes, there were significant differences between 2 groups from week 6 to week 11. The present study demonstrated that dynamic determination of CMV-DNA may predict the occurrence of CMV-IP. Viral loads over 10(4) copies/mL plasma continuing for 3 weeks may serve as a cutoff to predict CMV-IP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transplantation Proceedings
volume
36
issue
10
pages
3036 - 3041
publisher
Elsevier
external identifiers
  • pmid:15686689
  • wos:000226765800039
  • scopus:21244458875
ISSN
0041-1345
DOI
10.1016/j.transproceed.2004.10.050
language
English
LU publication?
yes
id
d1c5506f-d9da-4bbd-b9b6-c985b746f31f (old id 254028)
date added to LUP
2007-11-07 08:14:10
date last changed
2017-01-01 04:21:11
@article{d1c5506f-d9da-4bbd-b9b6-c985b746f31f,
  abstract     = {The present study prospectively examined the relationship between cytomegalovirus interstitial pneumonia (CMV-IP) and viral load among 56 renal transplant recipients. We sought to identify the cutoff of viral load to predict CMV-IP. Blood samples were obtained weekly within the first 2 months and every second week during 2 to 6 months after kidney transplantations. A commercial real-time polymerase chain reaction (PCR)-method was applied to quantify CMV-DNA in plasma or in leukocytes. Among 54 renal transplant recipients who were analyzed for CMV-DNA in the blood (96.4%), 8 experienced CMV-IP (14.3%) and 2 died (3.6%). After kidney transplantation, CMV-DNA loads were near 0 in plasma before the week 4 and before the week 3 in leukocytes among both groups. From week 5 (week 4, in leukocytes), plasma CMV-DNA loads in the CMV-IP group increased, the peak value reached at week 8 in plasma and the week 9 in leukocytes. Whereas, the CMV-DNA loads both in plasma and in leukocytes in the non-CMV-IP group fluctuated at lower levels, those in plasma were significantly different between the 2 groups at the weeks 5, 7, and 9. For CMV-DNA in leukocytes, there were significant differences between 2 groups from week 6 to week 11. The present study demonstrated that dynamic determination of CMV-DNA may predict the occurrence of CMV-IP. Viral loads over 10(4) copies/mL plasma continuing for 3 weeks may serve as a cutoff to predict CMV-IP.},
  author       = {Ye, Q and Luo, G and He, X and Zheng, W and Zheng, L and Dong, X and Xu, X and Nilsson-Ehle, Peter and Xu, Ning},
  issn         = {0041-1345},
  language     = {eng},
  number       = {10},
  pages        = {3036--3041},
  publisher    = {Elsevier},
  series       = {Transplantation Proceedings},
  title        = {Prospective study of relationship between cytomegalovirus pneumonia and viral load in renal transplant recipients},
  url          = {http://dx.doi.org/10.1016/j.transproceed.2004.10.050},
  volume       = {36},
  year         = {2004},
}