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Decorin deficiency leads to impaired angiogenesis in injured mouse cornea

Schonherr, E; Sunderkotter, C; Schaefer, L; Thanos, S; Grassel, S; Oldberg, Åke LU ; Iozzo, RV; Young, MF and Kresse, H (2004) In Journal of Vascular Research1992-01-01+01:00 41(6). p.499-508
Abstract
Small leucine-rich proteoglycans play important roles in the organization of the extracellular matrix as well as for the regulation of cell behavior; two biological processes that are essential for angiogenesis. We investigated consequences of the targeted ablation of decorin (DCN), biglycan (BGN) and fibromodulin (FMOD) genes on inflammation-induced angiogenesis in the cornea. In wildtype mice, DCN was localized exclusively to the corneal stroma, while FMOD and BGN were more prominently expressed in epithelial cells. Endothelial cells from limbus blood vessels expressed BGN and FMOD, but no DCN. However, after induction of angiogenesis by chemical cauterization, DCN was expressed in the newly formed capillaries, together with BGN and... (More)
Small leucine-rich proteoglycans play important roles in the organization of the extracellular matrix as well as for the regulation of cell behavior; two biological processes that are essential for angiogenesis. We investigated consequences of the targeted ablation of decorin (DCN), biglycan (BGN) and fibromodulin (FMOD) genes on inflammation-induced angiogenesis in the cornea. In wildtype mice, DCN was localized exclusively to the corneal stroma, while FMOD and BGN were more prominently expressed in epithelial cells. Endothelial cells from limbus blood vessels expressed BGN and FMOD, but no DCN. However, after induction of angiogenesis by chemical cauterization, DCN was expressed in the newly formed capillaries, together with BGN and FMOD. Notably, in DCN-deficient mice, the growth of vessels was significantly diminished, whereas it did not significantly change in FMOD- or BGN-deficient animals. Moreover, blood vessels of DCN-deficient mice exhibited a similar expression level of BGN as control mice, while FMOD was increased on day 3 after injury. These results indicate that DCN, in addition to its effects on fibrillogenesis, plays a regulatory role in angiogenesis and that FMOD in endothelial cells may be able to partially substitute for DCN. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
vascular endothelium, extracellular matrix, inflammation, collagen, corneal epithelium
in
Journal of Vascular Research1992-01-01+01:00
volume
41
issue
6
pages
499 - 508
publisher
Karger
external identifiers
  • wos:000226412300003
  • scopus:10644234507
ISSN
1423-0135
DOI
10.1159/000081806
language
English
LU publication?
yes
id
7cba926f-884c-4957-908c-899d8ec23d9d (old id 255169)
date added to LUP
2007-10-26 13:31:35
date last changed
2017-11-12 04:04:59
@article{7cba926f-884c-4957-908c-899d8ec23d9d,
  abstract     = {Small leucine-rich proteoglycans play important roles in the organization of the extracellular matrix as well as for the regulation of cell behavior; two biological processes that are essential for angiogenesis. We investigated consequences of the targeted ablation of decorin (DCN), biglycan (BGN) and fibromodulin (FMOD) genes on inflammation-induced angiogenesis in the cornea. In wildtype mice, DCN was localized exclusively to the corneal stroma, while FMOD and BGN were more prominently expressed in epithelial cells. Endothelial cells from limbus blood vessels expressed BGN and FMOD, but no DCN. However, after induction of angiogenesis by chemical cauterization, DCN was expressed in the newly formed capillaries, together with BGN and FMOD. Notably, in DCN-deficient mice, the growth of vessels was significantly diminished, whereas it did not significantly change in FMOD- or BGN-deficient animals. Moreover, blood vessels of DCN-deficient mice exhibited a similar expression level of BGN as control mice, while FMOD was increased on day 3 after injury. These results indicate that DCN, in addition to its effects on fibrillogenesis, plays a regulatory role in angiogenesis and that FMOD in endothelial cells may be able to partially substitute for DCN.},
  author       = {Schonherr, E and Sunderkotter, C and Schaefer, L and Thanos, S and Grassel, S and Oldberg, Åke and Iozzo, RV and Young, MF and Kresse, H},
  issn         = {1423-0135},
  keyword      = {vascular endothelium,extracellular matrix,inflammation,collagen,corneal epithelium},
  language     = {eng},
  number       = {6},
  pages        = {499--508},
  publisher    = {Karger},
  series       = {Journal of Vascular Research1992-01-01+01:00},
  title        = {Decorin deficiency leads to impaired angiogenesis in injured mouse cornea},
  url          = {http://dx.doi.org/10.1159/000081806},
  volume       = {41},
  year         = {2004},
}