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Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema

Ekberg-Jansson, A; Amin, K; Bake, B; Rosengren, A; Tylen, U; Venge, P and Löfdahl, Claes-Göran LU (2005) In Respiratory Medicine 99(1). p.75-83
Abstract
The pathologic mechanisms of chronic obstructive pulmonary disease (COPD) most certainly involves neutrophil granulocytes, cytotoxic T-cells, macophages and mast cells. The aim of this study was to investigate the relation between the number of mast cells in different compartments in bronchial biopsies of central proximal airways to structural changes, lung function tests and emphysema detected by high resolution computed tomography (HRCT). Twenty nine asymptomatic smoking and 16 never-smoking men from a population study were recruited. Central bronchial biopsies were stained to identify mast cells by immunohistochemistry. The number of mast cells in the epithelium, lamina propria and smooth muscle as welt as epithelial integrity and... (More)
The pathologic mechanisms of chronic obstructive pulmonary disease (COPD) most certainly involves neutrophil granulocytes, cytotoxic T-cells, macophages and mast cells. The aim of this study was to investigate the relation between the number of mast cells in different compartments in bronchial biopsies of central proximal airways to structural changes, lung function tests and emphysema detected by high resolution computed tomography (HRCT). Twenty nine asymptomatic smoking and 16 never-smoking men from a population study were recruited. Central bronchial biopsies were stained to identify mast cells by immunohistochemistry. The number of mast cells in the epithelium, lamina propria and smooth muscle as welt as epithelial integrity and thickness of the tenascin and laminin layer were determined. Smokers had increased numbers of mast cells in all compartments (P<0.001). Structural changes were correlated to mast cell numbers with the closest associations to mast cell numbers in the smooth muscle [epithelial integrity (R-S=-0.48, P=0.008), laminin layer (R-S=0.63, P=0.0002), tenascin layer (R-S=0.40, P=0.03)]. Similar correlations between mast cells and lung function tests were seen [functional residual capacity (FRC) (R-S=0.60, P=0.0006), total lung capacity (TLC) (R-S=0.44, P=0.02) and residual volume (RV) (R-S=0.41, P=0.03)].. No correlations could be detected between mast cells and FEV1 or to emphysema. Smoking is associated with an increase of mast cells in all compartments of the bronchial mucosa, including smooth muscle, and this is related to altered airway structure and function. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
high-resolution computed, lung function, chronic bronchitis, mast cells, tomography, respiratory symptoms
in
Respiratory Medicine
volume
99
issue
1
pages
75 - 83
publisher
Elsevier
external identifiers
  • wos:000226311100012
  • pmid:15672853
  • scopus:10944269808
ISSN
1532-3064
DOI
10.1016/j.rmed.2004.05.013
language
English
LU publication?
yes
id
61ae4d99-b964-4468-bd3f-2970816b83eb (old id 255552)
date added to LUP
2007-08-06 14:13:33
date last changed
2017-10-01 04:35:07
@article{61ae4d99-b964-4468-bd3f-2970816b83eb,
  abstract     = {The pathologic mechanisms of chronic obstructive pulmonary disease (COPD) most certainly involves neutrophil granulocytes, cytotoxic T-cells, macophages and mast cells. The aim of this study was to investigate the relation between the number of mast cells in different compartments in bronchial biopsies of central proximal airways to structural changes, lung function tests and emphysema detected by high resolution computed tomography (HRCT). Twenty nine asymptomatic smoking and 16 never-smoking men from a population study were recruited. Central bronchial biopsies were stained to identify mast cells by immunohistochemistry. The number of mast cells in the epithelium, lamina propria and smooth muscle as welt as epithelial integrity and thickness of the tenascin and laminin layer were determined. Smokers had increased numbers of mast cells in all compartments (P&lt;0.001). Structural changes were correlated to mast cell numbers with the closest associations to mast cell numbers in the smooth muscle [epithelial integrity (R-S=-0.48, P=0.008), laminin layer (R-S=0.63, P=0.0002), tenascin layer (R-S=0.40, P=0.03)]. Similar correlations between mast cells and lung function tests were seen [functional residual capacity (FRC) (R-S=0.60, P=0.0006), total lung capacity (TLC) (R-S=0.44, P=0.02) and residual volume (RV) (R-S=0.41, P=0.03)].. No correlations could be detected between mast cells and FEV1 or to emphysema. Smoking is associated with an increase of mast cells in all compartments of the bronchial mucosa, including smooth muscle, and this is related to altered airway structure and function.},
  author       = {Ekberg-Jansson, A and Amin, K and Bake, B and Rosengren, A and Tylen, U and Venge, P and Löfdahl, Claes-Göran},
  issn         = {1532-3064},
  keyword      = {high-resolution computed,lung function,chronic bronchitis,mast cells,tomography,respiratory symptoms},
  language     = {eng},
  number       = {1},
  pages        = {75--83},
  publisher    = {Elsevier},
  series       = {Respiratory Medicine},
  title        = {Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema},
  url          = {http://dx.doi.org/10.1016/j.rmed.2004.05.013},
  volume       = {99},
  year         = {2005},
}