A Syntenic Cross Species Aneuploidy Genetic Screen Links RCAN1 Expression to β-Cell Mitochondrial Dysfunction in Type 2 Diabetes
(2016) In PLoS Genetics 12(5).- Abstract
Type 2 diabetes (T2D) is a complex metabolic disease associated with obesity, insulin resistance and hypoinsulinemia due to pancreatic β-cell dysfunction. Reduced mitochondrial function is thought to be central to β-cell dysfunction. Mitochondrial dysfunction and reduced insulin secretion are also observed in β-cells of humans with the most common human genetic disorder, Down syndrome (DS, Trisomy 21). To identify regions of chromosome 21 that may be associated with perturbed glucose homeostasis we profiled the glycaemic status of different DS mouse models. The Ts65Dn and Dp16 DS mouse lines were hyperglycemic, while Tc1 and Ts1Rhr mice were not, providing us with a region of chromosome 21 containing genes that cause hyperglycemia. We... (More)
Type 2 diabetes (T2D) is a complex metabolic disease associated with obesity, insulin resistance and hypoinsulinemia due to pancreatic β-cell dysfunction. Reduced mitochondrial function is thought to be central to β-cell dysfunction. Mitochondrial dysfunction and reduced insulin secretion are also observed in β-cells of humans with the most common human genetic disorder, Down syndrome (DS, Trisomy 21). To identify regions of chromosome 21 that may be associated with perturbed glucose homeostasis we profiled the glycaemic status of different DS mouse models. The Ts65Dn and Dp16 DS mouse lines were hyperglycemic, while Tc1 and Ts1Rhr mice were not, providing us with a region of chromosome 21 containing genes that cause hyperglycemia. We then examined whether any of these genes were upregulated in a set of ~5,000 gene expression changes we had identified in a large gene expression analysis of human T2D β-cells. This approach produced a single gene, RCAN1, as a candidate gene linking hyperglycemia and functional changes in T2D β-cells. Further investigations demonstrated that RCAN1 methylation is reduced in human T2D islets at multiple sites, correlating with increased expression. RCAN1 protein expression was also increased in db/db mouse islets and in human and mouse islets exposed to high glucose. Mice overexpressing RCAN1 had reduced in vivo glucose-stimulated insulin secretion and their β-cells displayed mitochondrial dysfunction including hyperpolarised membrane potential, reduced oxidative phosphorylation and low ATP production. This lack of β-cell ATP had functional consequences by negatively affecting both glucose-stimulated membrane depolarisation and ATP-dependent insulin granule exocytosis. Thus, from amongst the myriad of gene expression changes occurring in T2D β-cells where we had little knowledge of which changes cause β-cell dysfunction, we applied a trisomy 21 screening approach which linked RCAN1 to β-cell mitochondrial dysfunction in T2D.
(Less)
- author
- Peiris, Heshan
; Duffield, Michael D.
; Fadista, Joao
LU
; Jessup, Claire F.
; Kashmir, Vinder
; Genders, Amanda J.
; McGee, Sean L.
; Martin, Alyce M.
; Saiedi, Madiha
; Morton, Nicholas
; Carter, Roderick
; Cousin, Michael A.
; Kokotos, Alexandros C.
; Oskolkov, Nikolay
LU
; Volkov, Petr
LU
; Hough, Tertius A.
; Fisher, Elizabeth M C
; Tybulewicz, Victor L J
; Busciglio, Jorge
; Coskun, Pinar E.
; Becker, Ann
; Belichenko, Pavel V.
; Mobley, William C.
; Ryan, Michael T.
; Chan, Jeng Yie
; Laybutt, D. Ross
; Coates, P. Toby
; Yang, Sijun
; Ling, Charlotte
LU
; Groop, Leif
LU
; Pritchard, Melanie A.
and Keating, Damien J.
(Less) - organization
- publishing date
- 2016-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS Genetics
- volume
- 12
- issue
- 5
- article number
- e1006033
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:27195491
- wos:000377197100034
- scopus:84974602795
- ISSN
- 1553-7390
- DOI
- 10.1371/journal.pgen.1006033
- language
- English
- LU publication?
- yes
- id
- 255954a2-08f3-46b5-a197-b2ffb34d41bc
- date added to LUP
- 2016-07-07 13:28:31
- date last changed
- 2024-04-05 03:35:10
@article{255954a2-08f3-46b5-a197-b2ffb34d41bc,
abstract = {{<p>Type 2 diabetes (T2D) is a complex metabolic disease associated with obesity, insulin resistance and hypoinsulinemia due to pancreatic β-cell dysfunction. Reduced mitochondrial function is thought to be central to β-cell dysfunction. Mitochondrial dysfunction and reduced insulin secretion are also observed in β-cells of humans with the most common human genetic disorder, Down syndrome (DS, Trisomy 21). To identify regions of chromosome 21 that may be associated with perturbed glucose homeostasis we profiled the glycaemic status of different DS mouse models. The Ts65Dn and Dp16 DS mouse lines were hyperglycemic, while Tc1 and Ts1Rhr mice were not, providing us with a region of chromosome 21 containing genes that cause hyperglycemia. We then examined whether any of these genes were upregulated in a set of ~5,000 gene expression changes we had identified in a large gene expression analysis of human T2D β-cells. This approach produced a single gene, RCAN1, as a candidate gene linking hyperglycemia and functional changes in T2D β-cells. Further investigations demonstrated that RCAN1 methylation is reduced in human T2D islets at multiple sites, correlating with increased expression. RCAN1 protein expression was also increased in db/db mouse islets and in human and mouse islets exposed to high glucose. Mice overexpressing RCAN1 had reduced in vivo glucose-stimulated insulin secretion and their β-cells displayed mitochondrial dysfunction including hyperpolarised membrane potential, reduced oxidative phosphorylation and low ATP production. This lack of β-cell ATP had functional consequences by negatively affecting both glucose-stimulated membrane depolarisation and ATP-dependent insulin granule exocytosis. Thus, from amongst the myriad of gene expression changes occurring in T2D β-cells where we had little knowledge of which changes cause β-cell dysfunction, we applied a trisomy 21 screening approach which linked RCAN1 to β-cell mitochondrial dysfunction in T2D.</p>}},
author = {{Peiris, Heshan and Duffield, Michael D. and Fadista, Joao and Jessup, Claire F. and Kashmir, Vinder and Genders, Amanda J. and McGee, Sean L. and Martin, Alyce M. and Saiedi, Madiha and Morton, Nicholas and Carter, Roderick and Cousin, Michael A. and Kokotos, Alexandros C. and Oskolkov, Nikolay and Volkov, Petr and Hough, Tertius A. and Fisher, Elizabeth M C and Tybulewicz, Victor L J and Busciglio, Jorge and Coskun, Pinar E. and Becker, Ann and Belichenko, Pavel V. and Mobley, William C. and Ryan, Michael T. and Chan, Jeng Yie and Laybutt, D. Ross and Coates, P. Toby and Yang, Sijun and Ling, Charlotte and Groop, Leif and Pritchard, Melanie A. and Keating, Damien J.}},
issn = {{1553-7390}},
language = {{eng}},
month = {{05}},
number = {{5}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS Genetics}},
title = {{A Syntenic Cross Species Aneuploidy Genetic Screen Links RCAN1 Expression to β-Cell Mitochondrial Dysfunction in Type 2 Diabetes}},
url = {{http://dx.doi.org/10.1371/journal.pgen.1006033}},
doi = {{10.1371/journal.pgen.1006033}},
volume = {{12}},
year = {{2016}},
}