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Triclosan lacks anti-estrogenic effects in Zebrafish cells but modulates estrogen response in Zebrafish embryos

Serra, Hélène LU ; Brion, François ; Porcher, Jean Marc ; Budzinski, Hélène and Aït-Aïssa, Selim (2018) In International Journal of Molecular Sciences 19(4).
Abstract

Triclosan (TCS), an antimicrobial agent widely found in the aquatic environment, is suspected to act as an endocrine disrupting compound, however mechanistic information is lacking in regards to aquatic species. This study assessed the ability of TCS to interfere with estrogen receptor (ER) transcriptional activity, in zebrafish-specific in vitro and in vivo reporter gene assays. We report that TCS exhibits a lack of either agonistic or antagonistic effects on a panel of ER-expressing zebrafish (ZELH-zfERα and -zfERβ) and human (MELN) cell lines. At the organism level, TCS at concentrations of up to 0.3 µM had no effect on ER-regulated brain aromatase gene expression in transgenic cyp19a1b-GFP zebrafish embryos. At a concentration of 1... (More)

Triclosan (TCS), an antimicrobial agent widely found in the aquatic environment, is suspected to act as an endocrine disrupting compound, however mechanistic information is lacking in regards to aquatic species. This study assessed the ability of TCS to interfere with estrogen receptor (ER) transcriptional activity, in zebrafish-specific in vitro and in vivo reporter gene assays. We report that TCS exhibits a lack of either agonistic or antagonistic effects on a panel of ER-expressing zebrafish (ZELH-zfERα and -zfERβ) and human (MELN) cell lines. At the organism level, TCS at concentrations of up to 0.3 µM had no effect on ER-regulated brain aromatase gene expression in transgenic cyp19a1b-GFP zebrafish embryos. At a concentration of 1 µM, TCS interfered with the E2 response in an ambivalent manner by potentializing a low E2 response (0.625 nM), but decreasing a high E2 response (10 nM). Altogether, our study suggests that while modulation of ER-regulated genes by TCS may occur in zebrafish, it does so irrespective of a direct binding and activation of zfERs.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Brain aromatase, Estrogen receptor, In vitro, In vivo, Triclosan, Zebrafish
in
International Journal of Molecular Sciences
volume
19
issue
4
article number
1175
publisher
MDPI AG
external identifiers
  • pmid:29649157
  • scopus:85045412108
ISSN
1661-6596
DOI
10.3390/ijms19041175
language
English
LU publication?
no
id
255c829e-4d99-45e7-a935-4146b12f72d4
date added to LUP
2019-05-02 10:53:58
date last changed
2024-04-16 03:39:38
@article{255c829e-4d99-45e7-a935-4146b12f72d4,
  abstract     = {{<p>Triclosan (TCS), an antimicrobial agent widely found in the aquatic environment, is suspected to act as an endocrine disrupting compound, however mechanistic information is lacking in regards to aquatic species. This study assessed the ability of TCS to interfere with estrogen receptor (ER) transcriptional activity, in zebrafish-specific in vitro and in vivo reporter gene assays. We report that TCS exhibits a lack of either agonistic or antagonistic effects on a panel of ER-expressing zebrafish (ZELH-zfERα and -zfERβ) and human (MELN) cell lines. At the organism level, TCS at concentrations of up to 0.3 µM had no effect on ER-regulated brain aromatase gene expression in transgenic cyp19a1b-GFP zebrafish embryos. At a concentration of 1 µM, TCS interfered with the E2 response in an ambivalent manner by potentializing a low E2 response (0.625 nM), but decreasing a high E2 response (10 nM). Altogether, our study suggests that while modulation of ER-regulated genes by TCS may occur in zebrafish, it does so irrespective of a direct binding and activation of zfERs.</p>}},
  author       = {{Serra, Hélène and Brion, François and Porcher, Jean Marc and Budzinski, Hélène and Aït-Aïssa, Selim}},
  issn         = {{1661-6596}},
  keywords     = {{Brain aromatase; Estrogen receptor; In vitro; In vivo; Triclosan; Zebrafish}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{4}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Triclosan lacks anti-estrogenic effects in Zebrafish cells but modulates estrogen response in Zebrafish embryos}},
  url          = {{http://dx.doi.org/10.3390/ijms19041175}},
  doi          = {{10.3390/ijms19041175}},
  volume       = {{19}},
  year         = {{2018}},
}