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Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique

Egesten, Arne LU ; Alumets, Jan LU ; von Mecklenburg, C ; Palmegren, M and Olsson, Inge LU (1986) In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 34(11). p.1399-1403
Abstract
An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
volume
34
issue
11
pages
1399 - 1403
publisher
Histochemical Society
external identifiers
  • scopus:0022466359
ISSN
1551-5044
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Faculty of Medicine (000022000), Respiratory Medicine and Allergology (013230111), Division of Infection Medicine (BMC) (013024020), Division of Hematology and Transfusion Medicine (013041100), Pathology, (Lund) (013030000)
id
2561d4ba-338d-4f8b-ace0-6872535659cc (old id 1296161)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/3772075
http://jhc.sagepub.com/content/34/11/1399.long
date added to LUP
2016-04-04 13:05:33
date last changed
2020-12-27 05:51:45
@article{2561d4ba-338d-4f8b-ace0-6872535659cc,
  abstract     = {{An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.}},
  author       = {{Egesten, Arne and Alumets, Jan and von Mecklenburg, C and Palmegren, M and Olsson, Inge}},
  issn         = {{1551-5044}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1399--1403}},
  publisher    = {{Histochemical Society}},
  series       = {{The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}},
  title        = {{Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique}},
  url          = {{http://www.ncbi.nlm.nih.gov/pubmed/3772075}},
  volume       = {{34}},
  year         = {{1986}},
}