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Mycobacteria-infected bystander macrophages trigger maturation of dendritic cells and enhance their ability to mediate HIV transinfection

Mazurek, Jolanta; Ignatowicz, Lech; Kallenius, Gunilla; Jansson, Marianne LU and Pawlowski, Andrzej (2012) In European Journal of Immunology 42(5). p.1192-1202
Abstract
Synergistic interplay between Mycobacterium tuberculosis (Mtb) and HIV in coinfected ind-ividuals leads to the acceleration of both tuberculosis and HIVdisease. Mtb, as well as HIV, may modulate the function of many immune cells, including DCs. To dissect the bystander impact of Mfs infected with Mtb on DC functionality, we here investigated changes in DC phenotype, cytokine profiles, and HIV-1 transinfecting ability. An in vitro system was used in which human monocyte-derived DCs were exposed to soluble factors released by Mfs infected with mycobacteria, including virulent clinical Mtb isolates and nonvirulent BCG. Soluble factors secreted from Mtb-infected Mfs, and to a lesser extent BCG-infected Mfs, resulted in the production of... (More)
Synergistic interplay between Mycobacterium tuberculosis (Mtb) and HIV in coinfected ind-ividuals leads to the acceleration of both tuberculosis and HIVdisease. Mtb, as well as HIV, may modulate the function of many immune cells, including DCs. To dissect the bystander impact of Mfs infected with Mtb on DC functionality, we here investigated changes in DC phenotype, cytokine profiles, and HIV-1 transinfecting ability. An in vitro system was used in which human monocyte-derived DCs were exposed to soluble factors released by Mfs infected with mycobacteria, including virulent clinical Mtb isolates and nonvirulent BCG. Soluble factors secreted from Mtb-infected Mfs, and to a lesser extent BCG-infected Mfs, resulted in the production of proinflammatory cytokines and partial upregulation of DC maturation markers. Interestingly, the HIV-1 transinfecting ability of DCs was enhanced upon exposure to soluble factors released by Mtb-infected Mfs. In summary, our study shows that DCs exposed to soluble factors released by mycobacteria-infected Mfs undergo maturation and display an augmented ability to transmit HIV-1 in trans. These findings highlight the important role of bystander effects during the course of MtbHIV coinfection and suggest that Mtb-infected Mfs may contribute to an environment that supports DC-mediated spread and amplification of HIV in coinfected individuals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
coinfection center dot dendritic cell center dot HIV-1 center dot, macrophage center dot Mycobacterium tuberculosis
in
European Journal of Immunology
volume
42
issue
5
pages
1192 - 1202
publisher
John Wiley & Sons
external identifiers
  • wos:000303317700016
  • scopus:84860318604
ISSN
1521-4141
DOI
10.1002/eji.201142049
language
English
LU publication?
yes
id
d53612ef-fe1e-4eab-b072-25cc5412e9a0 (old id 2563201)
date added to LUP
2012-06-01 08:48:48
date last changed
2017-03-21 10:14:02
@article{d53612ef-fe1e-4eab-b072-25cc5412e9a0,
  abstract     = {Synergistic interplay between Mycobacterium tuberculosis (Mtb) and HIV in coinfected ind-ividuals leads to the acceleration of both tuberculosis and HIVdisease. Mtb, as well as HIV, may modulate the function of many immune cells, including DCs. To dissect the bystander impact of Mfs infected with Mtb on DC functionality, we here investigated changes in DC phenotype, cytokine profiles, and HIV-1 transinfecting ability. An in vitro system was used in which human monocyte-derived DCs were exposed to soluble factors released by Mfs infected with mycobacteria, including virulent clinical Mtb isolates and nonvirulent BCG. Soluble factors secreted from Mtb-infected Mfs, and to a lesser extent BCG-infected Mfs, resulted in the production of proinflammatory cytokines and partial upregulation of DC maturation markers. Interestingly, the HIV-1 transinfecting ability of DCs was enhanced upon exposure to soluble factors released by Mtb-infected Mfs. In summary, our study shows that DCs exposed to soluble factors released by mycobacteria-infected Mfs undergo maturation and display an augmented ability to transmit HIV-1 in trans. These findings highlight the important role of bystander effects during the course of MtbHIV coinfection and suggest that Mtb-infected Mfs may contribute to an environment that supports DC-mediated spread and amplification of HIV in coinfected individuals.},
  author       = {Mazurek, Jolanta and Ignatowicz, Lech and Kallenius, Gunilla and Jansson, Marianne and Pawlowski, Andrzej},
  issn         = {1521-4141},
  keyword      = {coinfection center dot dendritic cell center dot HIV-1 center dot,macrophage center dot Mycobacterium tuberculosis},
  language     = {eng},
  number       = {5},
  pages        = {1192--1202},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {Mycobacteria-infected bystander macrophages trigger maturation of dendritic cells and enhance their ability to mediate HIV transinfection},
  url          = {http://dx.doi.org/10.1002/eji.201142049},
  volume       = {42},
  year         = {2012},
}