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Embryonic genome activation events in buffalo (Bubalus bubalis) preimplantation embryos

Verma, A.; Kumar, Parveen LU ; Rajput, S.; Roy, B.; De, S. and Datta, T. K. (2012) In Molecular Reproduction and Development 79(5). p.321-328
Abstract
Embryonic genome activation (EGA) is the first major step towards successful initiation of preimplantation development, which culminates in the formation of implantation-competent embryos. EGA occurs at species-specific embryonic cell stages. In the present work, EGA was identified for buffalo embryos by studying the development rate of embryos in normal as well as imposed transcription block conditions, analyzing bromo-uridine triphosphate (BrUTP) incorporation rates as evidence of de novo transcription initiation, and studying the expression status of eukaryotic translation initiation factor 1A (eIF1A), U2 auxiliary splicing factor (U2AF), and polyadenylate polymerase (PAP) genes at different embryonic cell stages. Under normal, in vitro... (More)
Embryonic genome activation (EGA) is the first major step towards successful initiation of preimplantation development, which culminates in the formation of implantation-competent embryos. EGA occurs at species-specific embryonic cell stages. In the present work, EGA was identified for buffalo embryos by studying the development rate of embryos in normal as well as imposed transcription block conditions, analyzing bromo-uridine triphosphate (BrUTP) incorporation rates as evidence of de novo transcription initiation, and studying the expression status of eukaryotic translation initiation factor 1A (eIF1A), U2 auxiliary splicing factor (U2AF), and polyadenylate polymerase (PAP) genes at different embryonic cell stages. Under normal, in vitro fertilization and culture conditions, about 26% and 17% of oocytes could reach morula and blastocyst stages, respectively, but no embryos could progress beyond 8-cell stages in presence of a-amanitin. Culturing embryos in the presence of BrUTP revealed a marked increase in its incorporation between 4- and 8-cell stages. All genes studied displayed an abrupt increase in expression between 4- and 8-cell stages; PAP expression was upregulated earlier from 2- to 4-cell stages. About 65% of PAP transcripts from the 4-cell stage and more than 70% of eIF1A, U2AF, and PAP transcripts at 8-cell stage embryos were found to be synthesized de novo. Together, these data suggest that a minor EGA in buffalo embryos happens from 2- to 4-cell stages, while the major EGA takes place from 4- to 8-cell stage transition. Mol. Reprod. Dev. 79: 321328, 2012. (C) 2012 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Reproduction and Development
volume
79
issue
5
pages
321 - 328
publisher
John Wiley & Sons
external identifiers
  • wos:000302863300003
  • scopus:84859841726
ISSN
1040-452X
DOI
10.1002/mrd.22027
language
English
LU publication?
yes
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d565340a-ffbe-4ecf-9c8b-6f33dbd8bbaf (old id 2570944)
date added to LUP
2012-06-04 09:57:04
date last changed
2017-01-01 03:19:35
@article{d565340a-ffbe-4ecf-9c8b-6f33dbd8bbaf,
  abstract     = {Embryonic genome activation (EGA) is the first major step towards successful initiation of preimplantation development, which culminates in the formation of implantation-competent embryos. EGA occurs at species-specific embryonic cell stages. In the present work, EGA was identified for buffalo embryos by studying the development rate of embryos in normal as well as imposed transcription block conditions, analyzing bromo-uridine triphosphate (BrUTP) incorporation rates as evidence of de novo transcription initiation, and studying the expression status of eukaryotic translation initiation factor 1A (eIF1A), U2 auxiliary splicing factor (U2AF), and polyadenylate polymerase (PAP) genes at different embryonic cell stages. Under normal, in vitro fertilization and culture conditions, about 26% and 17% of oocytes could reach morula and blastocyst stages, respectively, but no embryos could progress beyond 8-cell stages in presence of a-amanitin. Culturing embryos in the presence of BrUTP revealed a marked increase in its incorporation between 4- and 8-cell stages. All genes studied displayed an abrupt increase in expression between 4- and 8-cell stages; PAP expression was upregulated earlier from 2- to 4-cell stages. About 65% of PAP transcripts from the 4-cell stage and more than 70% of eIF1A, U2AF, and PAP transcripts at 8-cell stage embryos were found to be synthesized de novo. Together, these data suggest that a minor EGA in buffalo embryos happens from 2- to 4-cell stages, while the major EGA takes place from 4- to 8-cell stage transition. Mol. Reprod. Dev. 79: 321328, 2012. (C) 2012 Wiley Periodicals, Inc.},
  author       = {Verma, A. and Kumar, Parveen and Rajput, S. and Roy, B. and De, S. and Datta, T. K.},
  issn         = {1040-452X},
  language     = {eng},
  number       = {5},
  pages        = {321--328},
  publisher    = {John Wiley & Sons},
  series       = {Molecular Reproduction and Development},
  title        = {Embryonic genome activation events in buffalo (Bubalus bubalis) preimplantation embryos},
  url          = {http://dx.doi.org/10.1002/mrd.22027},
  volume       = {79},
  year         = {2012},
}