Identification of the minimal glycopeptide core recognized by T cells in a model for rheumatoid arthritis
(2005) In Bioorganic & Medicinal Chemistry 13(2). p.473-482- Abstract
- Collagen induced arthritis (CIA) is a common mouse model for rheumatoid arthritis. Two sets of truncated peptides derived from type II collagen have been prepared and tested for binding to A(q), a MHC-II molecule associated with development of CIA. Binding to A(q) correlated well with predictions from a computer-based model. T-cell hybridomas, obtained in CIA, were also used to study the ability of A(q) bound peptides to trigger a T-cell response. The minimal peptide epitope required for binding, as well as for giving a T-cell response, was determined to be CII260-267. In collagen this epitope is often glycosylated at hydroxylysine 264 and glycosylation has been shown to be an immunodominant feature in CIA. Synthesis and evaluation of... (More)
- Collagen induced arthritis (CIA) is a common mouse model for rheumatoid arthritis. Two sets of truncated peptides derived from type II collagen have been prepared and tested for binding to A(q), a MHC-II molecule associated with development of CIA. Binding to A(q) correlated well with predictions from a computer-based model. T-cell hybridomas, obtained in CIA, were also used to study the ability of A(q) bound peptides to trigger a T-cell response. The minimal peptide epitope required for binding, as well as for giving a T-cell response, was determined to be CII260-267. In collagen this epitope is often glycosylated at hydroxylysine 264 and glycosylation has been shown to be an immunodominant feature in CIA. Synthesis and evaluation of CII260-267 carrying a beta-D-galactosyl moiety at position 264 revealed that this glycopeptide stimulated representative members from a panel of carbohydrate-specific T-cell hybridomas obtained in CIA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/257653
- author
- Holm, L ; Kjellen, P ; Holmdahl, Rikard LU and Kihlberg, J
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- T cell, glycopeptide, collagen, rheumatoid arthritis
- in
- Bioorganic & Medicinal Chemistry
- volume
- 13
- issue
- 2
- pages
- 473 - 482
- publisher
- Elsevier
- external identifiers
-
- pmid:15598569
- wos:000226343800018
- scopus:10444240315
- ISSN
- 0968-0896
- DOI
- 10.1016/j.bmc.2004.10.011
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- 9961a15b-c306-4da8-a84f-047ef6846fdd (old id 257653)
- date added to LUP
- 2016-04-01 11:57:38
- date last changed
- 2022-01-26 20:44:01
@article{9961a15b-c306-4da8-a84f-047ef6846fdd,
abstract = {{Collagen induced arthritis (CIA) is a common mouse model for rheumatoid arthritis. Two sets of truncated peptides derived from type II collagen have been prepared and tested for binding to A(q), a MHC-II molecule associated with development of CIA. Binding to A(q) correlated well with predictions from a computer-based model. T-cell hybridomas, obtained in CIA, were also used to study the ability of A(q) bound peptides to trigger a T-cell response. The minimal peptide epitope required for binding, as well as for giving a T-cell response, was determined to be CII260-267. In collagen this epitope is often glycosylated at hydroxylysine 264 and glycosylation has been shown to be an immunodominant feature in CIA. Synthesis and evaluation of CII260-267 carrying a beta-D-galactosyl moiety at position 264 revealed that this glycopeptide stimulated representative members from a panel of carbohydrate-specific T-cell hybridomas obtained in CIA.}},
author = {{Holm, L and Kjellen, P and Holmdahl, Rikard and Kihlberg, J}},
issn = {{0968-0896}},
keywords = {{T cell; glycopeptide; collagen; rheumatoid arthritis}},
language = {{eng}},
number = {{2}},
pages = {{473--482}},
publisher = {{Elsevier}},
series = {{Bioorganic & Medicinal Chemistry}},
title = {{Identification of the minimal glycopeptide core recognized by T cells in a model for rheumatoid arthritis}},
url = {{http://dx.doi.org/10.1016/j.bmc.2004.10.011}},
doi = {{10.1016/j.bmc.2004.10.011}},
volume = {{13}},
year = {{2005}},
}