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Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow

Mansour, Anna; Abou-Ezzi, Grazia; Sitnicka Quinn, Ewa LU ; Jacobsen, Sten Eirik W LU ; Wakkach, Abdelilah and Blin-Wakkach, Claudine (2012) In Journal of Experimental Medicine 209(3). p.537-549
Abstract
Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM. Restoration of OCL activity reversed the defect in HSC niche formation. Our data demonstrate that OBLs are required for establishing HSC niches and... (More)
Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM. Restoration of OCL activity reversed the defect in HSC niche formation. Our data demonstrate that OBLs are required for establishing HSC niches and that osteoblastic development is induced by OCLs. These findings broaden our knowledge of the HSC niche formation, which is critical for understanding normal and pathological hematopoiesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Experimental Medicine
volume
209
issue
3
pages
537 - 549
publisher
Rockefeller University Press
external identifiers
  • wos:000302635900013
  • scopus:84860378826
ISSN
1540-9538
DOI
10.1084/jem.20110994
language
English
LU publication?
yes
id
63ad2720-b1b1-4e6f-920d-a3bbaf271717 (old id 2580949)
date added to LUP
2012-06-01 09:35:19
date last changed
2017-11-12 03:47:31
@article{63ad2720-b1b1-4e6f-920d-a3bbaf271717,
  abstract     = {Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM. Restoration of OCL activity reversed the defect in HSC niche formation. Our data demonstrate that OBLs are required for establishing HSC niches and that osteoblastic development is induced by OCLs. These findings broaden our knowledge of the HSC niche formation, which is critical for understanding normal and pathological hematopoiesis.},
  author       = {Mansour, Anna and Abou-Ezzi, Grazia and Sitnicka Quinn, Ewa and Jacobsen, Sten Eirik W and Wakkach, Abdelilah and Blin-Wakkach, Claudine},
  issn         = {1540-9538},
  language     = {eng},
  number       = {3},
  pages        = {537--549},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Experimental Medicine},
  title        = {Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow},
  url          = {http://dx.doi.org/10.1084/jem.20110994},
  volume       = {209},
  year         = {2012},
}