Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow
(2012) In Journal of Experimental Medicine 209(3). p.537-549- Abstract
- Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM. Restoration of OCL activity reversed the defect in HSC niche formation. Our data demonstrate that OBLs are required for establishing HSC niches and... (More)
- Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM. Restoration of OCL activity reversed the defect in HSC niche formation. Our data demonstrate that OBLs are required for establishing HSC niches and that osteoblastic development is induced by OCLs. These findings broaden our knowledge of the HSC niche formation, which is critical for understanding normal and pathological hematopoiesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2580949
- author
- Mansour, Anna ; Abou-Ezzi, Grazia ; Sitnicka Quinn, Ewa LU ; Jacobsen, Sten Eirik W LU ; Wakkach, Abdelilah and Blin-Wakkach, Claudine
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Experimental Medicine
- volume
- 209
- issue
- 3
- pages
- 537 - 549
- publisher
- Rockefeller University Press
- external identifiers
-
- wos:000302635900013
- scopus:84860378826
- pmid:22351931
- ISSN
- 1540-9538
- DOI
- 10.1084/jem.20110994
- language
- English
- LU publication?
- yes
- id
- 63ad2720-b1b1-4e6f-920d-a3bbaf271717 (old id 2580949)
- date added to LUP
- 2016-04-01 14:43:02
- date last changed
- 2022-08-29 17:21:20
@article{63ad2720-b1b1-4e6f-920d-a3bbaf271717, abstract = {{Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM. Restoration of OCL activity reversed the defect in HSC niche formation. Our data demonstrate that OBLs are required for establishing HSC niches and that osteoblastic development is induced by OCLs. These findings broaden our knowledge of the HSC niche formation, which is critical for understanding normal and pathological hematopoiesis.}}, author = {{Mansour, Anna and Abou-Ezzi, Grazia and Sitnicka Quinn, Ewa and Jacobsen, Sten Eirik W and Wakkach, Abdelilah and Blin-Wakkach, Claudine}}, issn = {{1540-9538}}, language = {{eng}}, number = {{3}}, pages = {{537--549}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Experimental Medicine}}, title = {{Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow}}, url = {{https://lup.lub.lu.se/search/files/4124117/3127292.pdf}}, doi = {{10.1084/jem.20110994}}, volume = {{209}}, year = {{2012}}, }