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Self-Organization of Motor-Propelled Cytoskeletal Filaments at Topographically Defined Borders

Mansson, Alf; Bunk, Richard LU ; Sundberg, Mark and Montelius, Lars LU (2012) In Journal of Biomedicine and Biotechnology
Abstract
Self-organization phenomena are of critical importance in living organisms and of great interest to exploit in nanotechnology. Here we describe in vitro self-organization of molecular motor-propelled actin filaments, manifested as a tendency of the filaments to accumulate in high density close to topographically defined edges on nano- and microstructured surfaces. We hypothesized that this "edge-tracing" effect either (1) results from increased motor density along the guiding edges or (2) is a direct consequence of the asymmetric constraints on stochastic changes in filament sliding direction imposed by the edges. The latter hypothesis is well captured by a model explicitly defining the constraints of motility on structured surfaces in... (More)
Self-organization phenomena are of critical importance in living organisms and of great interest to exploit in nanotechnology. Here we describe in vitro self-organization of molecular motor-propelled actin filaments, manifested as a tendency of the filaments to accumulate in high density close to topographically defined edges on nano- and microstructured surfaces. We hypothesized that this "edge-tracing" effect either (1) results from increased motor density along the guiding edges or (2) is a direct consequence of the asymmetric constraints on stochastic changes in filament sliding direction imposed by the edges. The latter hypothesis is well captured by a model explicitly defining the constraints of motility on structured surfaces in combination with Monte-Carlo simulations [cf. Nitta et al. (2006)] of filament sliding. In support of hypothesis 2 we found that the model reproduced the edge tracing effect without the need to assume increased motor density at the edges. We then used model simulations to elucidate mechanistic details. The results are discussed in relation to nanotechnological applications and future experiments to test model predictions. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Biomedicine and Biotechnology
publisher
Hindawi Publishing Corporation
external identifiers
  • wos:000302684600001
  • scopus:84859733273
ISSN
1110-7251
DOI
10.1155/2012/647265
language
English
LU publication?
yes
id
430191ae-3b51-42e4-9e87-2971422f693a (old id 2587326)
date added to LUP
2012-06-04 12:50:44
date last changed
2017-08-20 03:20:13
@article{430191ae-3b51-42e4-9e87-2971422f693a,
  abstract     = {Self-organization phenomena are of critical importance in living organisms and of great interest to exploit in nanotechnology. Here we describe in vitro self-organization of molecular motor-propelled actin filaments, manifested as a tendency of the filaments to accumulate in high density close to topographically defined edges on nano- and microstructured surfaces. We hypothesized that this "edge-tracing" effect either (1) results from increased motor density along the guiding edges or (2) is a direct consequence of the asymmetric constraints on stochastic changes in filament sliding direction imposed by the edges. The latter hypothesis is well captured by a model explicitly defining the constraints of motility on structured surfaces in combination with Monte-Carlo simulations [cf. Nitta et al. (2006)] of filament sliding. In support of hypothesis 2 we found that the model reproduced the edge tracing effect without the need to assume increased motor density at the edges. We then used model simulations to elucidate mechanistic details. The results are discussed in relation to nanotechnological applications and future experiments to test model predictions.},
  articleno    = {647265},
  author       = {Mansson, Alf and Bunk, Richard and Sundberg, Mark and Montelius, Lars},
  issn         = {1110-7251},
  language     = {eng},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Journal of Biomedicine and Biotechnology},
  title        = {Self-Organization of Motor-Propelled Cytoskeletal Filaments at Topographically Defined Borders},
  url          = {http://dx.doi.org/10.1155/2012/647265},
  year         = {2012},
}