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Interaction of Ferrocene Mediators with Gluconobacter oxydans Immobilized Whole Cells and Membrane Fractions in Oxidation of Ethanol

Indzhgiya, E. Yu; Ponamoreva, O. N.; Alferov, V. A.; Reshetilov, A. N. and Gorton, Lo LU (2012) In Electroanalysis 24(4). p.924-930
Abstract
Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the... (More)
Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the reference data. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bioelectrocatalysis, Ferrocene mediators, Gluconobacter oxydans, Membrane fractions
in
Electroanalysis
volume
24
issue
4
pages
924 - 930
publisher
John Wiley & Sons
external identifiers
  • wos:000302160000027
  • scopus:84859241227
ISSN
1040-0397
DOI
10.1002/elan.201100425
language
English
LU publication?
yes
id
860f742f-4424-42cd-a128-f63392d9467f (old id 2587623)
date added to LUP
2012-05-30 15:52:41
date last changed
2017-03-26 03:53:36
@article{860f742f-4424-42cd-a128-f63392d9467f,
  abstract     = {Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the reference data.},
  author       = {Indzhgiya, E. Yu and Ponamoreva, O. N. and Alferov, V. A. and Reshetilov, A. N. and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {Bioelectrocatalysis,Ferrocene mediators,Gluconobacter oxydans,Membrane fractions},
  language     = {eng},
  number       = {4},
  pages        = {924--930},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Interaction of Ferrocene Mediators with Gluconobacter oxydans Immobilized Whole Cells and Membrane Fractions in Oxidation of Ethanol},
  url          = {http://dx.doi.org/10.1002/elan.201100425},
  volume       = {24},
  year         = {2012},
}