A single-cell transcriptomic study of heterogeneity in human embryonic tanycytes
(2024) In Scientific Reports 14(1).- Abstract
Disruptions in energy homeostasis can lead to diseases like obesity and diabetes, affecting millions of people each year. Tanycytes, the adult stem cells in the hypothalamus, play crucial roles in assisting hypothalamic neurons in maintaining energy balance. Although tanycytes have been extensively studied in rodents, our understanding of human tanycytes remains limited. In this study, we utilized single-cell transcriptomics data to explore the heterogeneity of human embryonic tanycytes, investigate their gene regulatory networks, analyze their intercellular communication, and examine their developmental trajectory. Our analysis revealed the presence of two clusters of β tanycytes and three clusters of α tanycytes in our dataset.... (More)
Disruptions in energy homeostasis can lead to diseases like obesity and diabetes, affecting millions of people each year. Tanycytes, the adult stem cells in the hypothalamus, play crucial roles in assisting hypothalamic neurons in maintaining energy balance. Although tanycytes have been extensively studied in rodents, our understanding of human tanycytes remains limited. In this study, we utilized single-cell transcriptomics data to explore the heterogeneity of human embryonic tanycytes, investigate their gene regulatory networks, analyze their intercellular communication, and examine their developmental trajectory. Our analysis revealed the presence of two clusters of β tanycytes and three clusters of α tanycytes in our dataset. Surprisingly, human embryonic tanycytes displayed significant similarities to mouse tanycytes in terms of marker gene expression and transcription factor activities. Trajectory analysis indicated that α tanycytes were the first to be generated, giving rise to β tanycytes in a dorsal–ventral direction along the third ventricle. Furthermore, our CellChat analyses demonstrated that tanycytes generated earlier along the developmental lineages exhibited increased intercellular communication compared to those generated later. In summary, we have thoroughly characterized the heterogeneity of human embryonic tanycytes from various angles. We are confident that our findings will serve as a foundation for future research on human tanycytes.
(Less)
- author
- Bai, Yiguang ; Chen, Qiaoling and Li, Yuan LU
- organization
- publishing date
- 2024-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell–cell communication, Developmental trajectory, Heterogeneity, Human embryonic tanycyte, Intercellular communication, Regulon, Single cell transcriptomics, Transcription factor
- in
- Scientific Reports
- volume
- 14
- issue
- 1
- article number
- 15384
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85197547058
- pmid:38965316
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-024-66044-7
- language
- English
- LU publication?
- yes
- id
- 258850a2-cb52-49e7-a164-972607bd4c20
- date added to LUP
- 2024-08-30 14:19:19
- date last changed
- 2024-08-31 03:00:16
@article{258850a2-cb52-49e7-a164-972607bd4c20,
abstract = {{<p>Disruptions in energy homeostasis can lead to diseases like obesity and diabetes, affecting millions of people each year. Tanycytes, the adult stem cells in the hypothalamus, play crucial roles in assisting hypothalamic neurons in maintaining energy balance. Although tanycytes have been extensively studied in rodents, our understanding of human tanycytes remains limited. In this study, we utilized single-cell transcriptomics data to explore the heterogeneity of human embryonic tanycytes, investigate their gene regulatory networks, analyze their intercellular communication, and examine their developmental trajectory. Our analysis revealed the presence of two clusters of β tanycytes and three clusters of α tanycytes in our dataset. Surprisingly, human embryonic tanycytes displayed significant similarities to mouse tanycytes in terms of marker gene expression and transcription factor activities. Trajectory analysis indicated that α tanycytes were the first to be generated, giving rise to β tanycytes in a dorsal–ventral direction along the third ventricle. Furthermore, our CellChat analyses demonstrated that tanycytes generated earlier along the developmental lineages exhibited increased intercellular communication compared to those generated later. In summary, we have thoroughly characterized the heterogeneity of human embryonic tanycytes from various angles. We are confident that our findings will serve as a foundation for future research on human tanycytes.</p>}},
author = {{Bai, Yiguang and Chen, Qiaoling and Li, Yuan}},
issn = {{2045-2322}},
keywords = {{Cell–cell communication; Developmental trajectory; Heterogeneity; Human embryonic tanycyte; Intercellular communication; Regulon; Single cell transcriptomics; Transcription factor}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{A single-cell transcriptomic study of heterogeneity in human embryonic tanycytes}},
url = {{http://dx.doi.org/10.1038/s41598-024-66044-7}},
doi = {{10.1038/s41598-024-66044-7}},
volume = {{14}},
year = {{2024}},
}