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MiR-129-5p is required for histone deacetylase inhibitor-induced cell death in thyroid cancer cells

Brest, Patrick; Lassalle, Sandra; Hofman, Veronique; Bordone, Olivier; Tanga, Virginie Gavric; Bonnetaud, Christelle; Moreilhon, Chimene; Rios, Geraldine; Santini, Jose and Barbry, Pascal, et al. (2011) In Endocrine-Related Cancer 18(6). p.711-719
Abstract
The molecular mechanism responsible for the antitumor activity of histone deacetylase inhibitors (HDACi) remains elusive. As HDACi have been described to alter miRNA expression, the aim of this study was to characterize HDACi-induced miRNAs and to determine their functional importance in the induction of cell death alone or in combination with other cancer drugs. Two HDACi, trichostatin A and vorinostat, induced miR-129-5p overexpression, histone acetylation and cell death in BCPAP, TPC-1, 8505C, and CAL62 cell lines and in primary cultures of papillary thyroid cancer (PTC) cells. In addition, miR-129-5p alone was sufficient to induce cell death and knockdown experiments showed that expression of this miRNA was required for HDACi-induced... (More)
The molecular mechanism responsible for the antitumor activity of histone deacetylase inhibitors (HDACi) remains elusive. As HDACi have been described to alter miRNA expression, the aim of this study was to characterize HDACi-induced miRNAs and to determine their functional importance in the induction of cell death alone or in combination with other cancer drugs. Two HDACi, trichostatin A and vorinostat, induced miR-129-5p overexpression, histone acetylation and cell death in BCPAP, TPC-1, 8505C, and CAL62 cell lines and in primary cultures of papillary thyroid cancer (PTC) cells. In addition, miR-129-5p alone was sufficient to induce cell death and knockdown experiments showed that expression of this miRNA was required for HDACi-induced cell death. Moreover, miR-129-5p accentuated the anti-proliferative effects of other cancer drugs such as etoposide or human a-lactalbumin made lethal for tumor cells (HAMLET). Taken together, our data show that miR-129-5p is involved in the antitumor activity of HDACi and highlight a miRNA-driven cell death mechanism. Endocrine-Related Cancer (2011) 18 711-719 (Less)
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publication status
published
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Endocrine-Related Cancer
volume
18
issue
6
pages
711 - 719
publisher
Society for Endocrinology
external identifiers
  • wos:000302242800013
  • scopus:84855960713
ISSN
1479-6821
DOI
10.1530/ERC-10-0257
language
English
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yes
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dd9d2467-4e06-4e56-bb58-2d9494429c3e (old id 2594782)
date added to LUP
2012-06-01 09:39:24
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2017-11-12 03:03:40
@article{dd9d2467-4e06-4e56-bb58-2d9494429c3e,
  abstract     = {The molecular mechanism responsible for the antitumor activity of histone deacetylase inhibitors (HDACi) remains elusive. As HDACi have been described to alter miRNA expression, the aim of this study was to characterize HDACi-induced miRNAs and to determine their functional importance in the induction of cell death alone or in combination with other cancer drugs. Two HDACi, trichostatin A and vorinostat, induced miR-129-5p overexpression, histone acetylation and cell death in BCPAP, TPC-1, 8505C, and CAL62 cell lines and in primary cultures of papillary thyroid cancer (PTC) cells. In addition, miR-129-5p alone was sufficient to induce cell death and knockdown experiments showed that expression of this miRNA was required for HDACi-induced cell death. Moreover, miR-129-5p accentuated the anti-proliferative effects of other cancer drugs such as etoposide or human a-lactalbumin made lethal for tumor cells (HAMLET). Taken together, our data show that miR-129-5p is involved in the antitumor activity of HDACi and highlight a miRNA-driven cell death mechanism. Endocrine-Related Cancer (2011) 18 711-719},
  author       = {Brest, Patrick and Lassalle, Sandra and Hofman, Veronique and Bordone, Olivier and Tanga, Virginie Gavric and Bonnetaud, Christelle and Moreilhon, Chimene and Rios, Geraldine and Santini, Jose and Barbry, Pascal and Svanborg, Catharina and Mograbi, Baharia and Mari, Bernard and Hofman, Paul},
  issn         = {1479-6821},
  language     = {eng},
  number       = {6},
  pages        = {711--719},
  publisher    = {Society for Endocrinology},
  series       = {Endocrine-Related Cancer},
  title        = {MiR-129-5p is required for histone deacetylase inhibitor-induced cell death in thyroid cancer cells},
  url          = {http://dx.doi.org/10.1530/ERC-10-0257},
  volume       = {18},
  year         = {2011},
}