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Efficient purification and morphology characterization of paclitaxel from cell cultures of Taxus chinensis

Pyo, Sang-Hyun LU orcid ; Kim, Moon‐Suk ; Cho, Jin-Suk ; Song, Bong-Kyu ; Han, Byung-Hee and Choi, Ho-Joon (2004) In Journal of Chemical Technology and Biotechnology 79(10). p.1162-1168
Abstract
Paclitaxel was purified from cell cultures of Taxus chinensis by a
combination of extraction, low-pressure chromatography, precipitation
and HPLC. A crude extract (purity 6.9%) was obtained by methanol
extraction of plant cell cultures, followed by liquid–liquid extraction
using dichloromethane. The extract was purified to greater than 32% with
a 97% step yield by low-pressure chromatography. After acetone/pentane
precipitation, the resulting purity and step yield were 75.8% and 97.4%,
respectively. High performance liquid chromatography steps, which were
composed of an HPLC step with column-packed ODS and an HPLC step with
column-packed silica, were applied to give over 99.5% purity with... (More)
Paclitaxel was purified from cell cultures of Taxus chinensis by a
combination of extraction, low-pressure chromatography, precipitation
and HPLC. A crude extract (purity 6.9%) was obtained by methanol
extraction of plant cell cultures, followed by liquid–liquid extraction
using dichloromethane. The extract was purified to greater than 32% with
a 97% step yield by low-pressure chromatography. After acetone/pentane
precipitation, the resulting purity and step yield were 75.8% and 97.4%,
respectively. High performance liquid chromatography steps, which were
composed of an HPLC step with column-packed ODS and an HPLC step with
column-packed silica, were applied to give over 99.5% purity with high
yield. Amorphous paclitaxel with a fine particle size, which has a
solubility advantage compared with the stable crystalline form, was
obtained by dissolving in dichloromethane, followed by spray-drying. (Less)
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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Journal of Chemical Technology and Biotechnology
volume
79
issue
10
pages
1162 - 1168
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:5044231912
ISSN
0268-2575
DOI
10.1002/jctb.1111
language
English
LU publication?
no
id
25e33ce1-5413-49a0-bf93-53285a98d104
date added to LUP
2025-09-05 17:59:39
date last changed
2025-10-14 12:49:06
@article{25e33ce1-5413-49a0-bf93-53285a98d104,
  abstract     = {{Paclitaxel was purified from cell cultures of <i>Taxus chinensis</i> by a<br>
 combination of extraction, low-pressure chromatography, precipitation <br>
and HPLC. A crude extract (purity 6.9%) was obtained by methanol <br>
extraction of plant cell cultures, followed by liquid–liquid extraction <br>
using dichloromethane. The extract was purified to greater than 32% with<br>
 a 97% step yield by low-pressure chromatography. After acetone/pentane <br>
precipitation, the resulting purity and step yield were 75.8% and 97.4%,<br>
 respectively. High performance liquid chromatography steps, which were <br>
composed of an HPLC step with column-packed ODS and an HPLC step with <br>
column-packed silica, were applied to give over 99.5% purity with high <br>
yield. Amorphous paclitaxel with a fine particle size, which has a <br>
solubility advantage compared with the stable crystalline form, was <br>
obtained by dissolving in dichloromethane, followed by spray-drying.}},
  author       = {{Pyo, Sang-Hyun and Kim, Moon‐Suk and Cho, Jin-Suk and Song, Bong-Kyu and Han, Byung-Hee and Choi, Ho-Joon}},
  issn         = {{0268-2575}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1162--1168}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Chemical Technology and Biotechnology}},
  title        = {{Efficient purification and morphology characterization of paclitaxel from cell cultures of <i>Taxus chinensis</i>}},
  url          = {{http://dx.doi.org/10.1002/jctb.1111}},
  doi          = {{10.1002/jctb.1111}},
  volume       = {{79}},
  year         = {{2004}},
}