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Electrocatalytically active multi-protein assemblies using nanoscaled building blocks

Feifel, Sven C. ; Kapp, Andreas ; Ludwig, Roland ; Gorton, Lo LU and Lisdat, Fred (2013) In RSC Advances 3(10). p.3428-3437
Abstract
Biosensors based on nanomaterials constitute an emerging area of interdisciplinary research. In particular in electrochemical sensors, electron transfer cascades can be used for defined signal generation. Our study describes the investigation of silica nanoparticles (SiNPs), DNA, cytochrome c (cyt c) and cellobiose dehydrogenase (CDH) for the development of catalytically active multi-protein assemblies. We report on direct and interprotein electron transfer reaction cascades of CDH and cyt c in an immobilized form by means of nanoscaled building blocks: Carboxy-modified SiNPs, and DNA. The building blocks provide an artificial matrix, which permit protein arrangement in an electro- and catalytically-active form. Multilayered protein... (More)
Biosensors based on nanomaterials constitute an emerging area of interdisciplinary research. In particular in electrochemical sensors, electron transfer cascades can be used for defined signal generation. Our study describes the investigation of silica nanoparticles (SiNPs), DNA, cytochrome c (cyt c) and cellobiose dehydrogenase (CDH) for the development of catalytically active multi-protein assemblies. We report on direct and interprotein electron transfer reaction cascades of CDH and cyt c in an immobilized form by means of nanoscaled building blocks: Carboxy-modified SiNPs, and DNA. The building blocks provide an artificial matrix, which permit protein arrangement in an electro- and catalytically-active form. Multilayered protein architectures on electrodes featuring direct and interprotein electron transfer by the use of entirely different nanoscaled building blocks has been established for the first time. In addition we highlight, that the secondary building blocks (DNA or SiNPs) used for the construction as well as the glycosylation of the enzyme (CDH) play a key role for the mode of operation in such complex entities. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
RSC Advances
volume
3
issue
10
pages
3428 - 3437
publisher
Royal Society of Chemistry
external identifiers
  • wos:000314701800037
  • scopus:84873679507
ISSN
2046-2069
DOI
10.1039/c2ra22819j
language
English
LU publication?
yes
id
25f3d082-5763-4a0e-a20e-73ae879eccd3 (old id 3590110)
date added to LUP
2016-04-01 13:03:19
date last changed
2023-09-02 18:06:36
@article{25f3d082-5763-4a0e-a20e-73ae879eccd3,
  abstract     = {{Biosensors based on nanomaterials constitute an emerging area of interdisciplinary research. In particular in electrochemical sensors, electron transfer cascades can be used for defined signal generation. Our study describes the investigation of silica nanoparticles (SiNPs), DNA, cytochrome c (cyt c) and cellobiose dehydrogenase (CDH) for the development of catalytically active multi-protein assemblies. We report on direct and interprotein electron transfer reaction cascades of CDH and cyt c in an immobilized form by means of nanoscaled building blocks: Carboxy-modified SiNPs, and DNA. The building blocks provide an artificial matrix, which permit protein arrangement in an electro- and catalytically-active form. Multilayered protein architectures on electrodes featuring direct and interprotein electron transfer by the use of entirely different nanoscaled building blocks has been established for the first time. In addition we highlight, that the secondary building blocks (DNA or SiNPs) used for the construction as well as the glycosylation of the enzyme (CDH) play a key role for the mode of operation in such complex entities.}},
  author       = {{Feifel, Sven C. and Kapp, Andreas and Ludwig, Roland and Gorton, Lo and Lisdat, Fred}},
  issn         = {{2046-2069}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{3428--3437}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{RSC Advances}},
  title        = {{Electrocatalytically active multi-protein assemblies using nanoscaled building blocks}},
  url          = {{http://dx.doi.org/10.1039/c2ra22819j}},
  doi          = {{10.1039/c2ra22819j}},
  volume       = {{3}},
  year         = {{2013}},
}