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High frequency of false-positive reactions in attempted patch testing with acrylate/methacrylate mixes.

Goon, Anthony LU ; Bruze, Magnus LU ; Zimerson, Erik LU ; Goossens, An; Goh, Chee-Leok and Isaksson, Marléne LU (2012) In Contact Dermatitis 67(3). p.157-161
Abstract
Background:

Although acrylate/methacrylate allergy has been frequently reported, until now patch testing with this group of allergens has been unwieldy, requiring the application of large supplementary series in most centres.



Objectives:

To formulate and evaluate two mixes of acrylate/methacrylate allergens in three centres (Malmö, Singapore, and Leuven).



Patients/materials/methods:

All patients tested with the baseline series during the study period were also patch tested with the mixes. Mix 1 consisted of: triethyleneglycol diacrylate (TREGDA) 0.1% wt/wt, 2-hydroxyethyl methacrylate (2-HEMA) 1.0% wt/wt and ethyleneglycol dimethacrylate 1.0% wt/wt in petrolatum. Mix 2... (More)
Background:

Although acrylate/methacrylate allergy has been frequently reported, until now patch testing with this group of allergens has been unwieldy, requiring the application of large supplementary series in most centres.



Objectives:

To formulate and evaluate two mixes of acrylate/methacrylate allergens in three centres (Malmö, Singapore, and Leuven).



Patients/materials/methods:

All patients tested with the baseline series during the study period were also patch tested with the mixes. Mix 1 consisted of: triethyleneglycol diacrylate (TREGDA) 0.1% wt/wt, 2-hydroxyethyl methacrylate (2-HEMA) 1.0% wt/wt and ethyleneglycol dimethacrylate 1.0% wt/wt in petrolatum. Mix 2 consisted of: TREGDA 0.1% wt/wt and 2-HEMA 2.0% wt/wt in pet. The separate components of the two mixes were also tested simultaneously. Results. There were 25 (5 males; 20 females) positive reactions to mix 1 with 16 in Malmö, 8 in Singapore, and 1 in Leuven. Positive reactions to mix 2 were seen only in Malmö, in 8 female patients. Thus, the positive reaction rate for mix 1 was 8.3% overall (Malmö 7.7%, Singapore 18.6%, and Leuven 2.1%), and that for mix 2 was 2.7% overall (Malmö 3.8%, Singapore 0%, and Leuven 0%). Of the 16 positive reactions to mix 1 in Malmö, only 4 were considered to be true allergic reactions, as the component allergen testing gave totally negative results in 12/16. For mix 2, only 3/8 positive reactions were considered to be true allergic reactions, as the component testing was negative in 5/8. Many doubtful (10-20%) and positive but non-allergic reactions were recorded, leading to early termination of the study.



Conclusions:

Although this was an unsuccessful attempt to formulate an acrylate/methacrylate mix, our experience will be useful for those embarking on future attempts to do this. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Contact Dermatitis
volume
67
issue
3
pages
157 - 161
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000307846500006
  • pmid:22624961
  • scopus:84865246727
ISSN
0105-1873
DOI
10.1111/j.1600-0536.2012.02106.x
language
English
LU publication?
yes
id
23974221-26c4-4205-b457-0c8e3840970c (old id 2608519)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22624961?dopt=Abstract
date added to LUP
2012-06-03 20:29:38
date last changed
2017-02-05 04:31:57
@article{23974221-26c4-4205-b457-0c8e3840970c,
  abstract     = {Background:<br/><br>
Although acrylate/methacrylate allergy has been frequently reported, until now patch testing with this group of allergens has been unwieldy, requiring the application of large supplementary series in most centres. <br/><br>
<br/><br>
Objectives:<br/><br>
To formulate and evaluate two mixes of acrylate/methacrylate allergens in three centres (Malmö, Singapore, and Leuven). <br/><br>
<br/><br>
Patients/materials/methods:<br/><br>
All patients tested with the baseline series during the study period were also patch tested with the mixes. Mix 1 consisted of: triethyleneglycol diacrylate (TREGDA) 0.1% wt/wt, 2-hydroxyethyl methacrylate (2-HEMA) 1.0% wt/wt and ethyleneglycol dimethacrylate 1.0% wt/wt in petrolatum. Mix 2 consisted of: TREGDA 0.1% wt/wt and 2-HEMA 2.0% wt/wt in pet. The separate components of the two mixes were also tested simultaneously. Results. There were 25 (5 males; 20 females) positive reactions to mix 1 with 16 in Malmö, 8 in Singapore, and 1 in Leuven. Positive reactions to mix 2 were seen only in Malmö, in 8 female patients. Thus, the positive reaction rate for mix 1 was 8.3% overall (Malmö 7.7%, Singapore 18.6%, and Leuven 2.1%), and that for mix 2 was 2.7% overall (Malmö 3.8%, Singapore 0%, and Leuven 0%). Of the 16 positive reactions to mix 1 in Malmö, only 4 were considered to be true allergic reactions, as the component allergen testing gave totally negative results in 12/16. For mix 2, only 3/8 positive reactions were considered to be true allergic reactions, as the component testing was negative in 5/8. Many doubtful (10-20%) and positive but non-allergic reactions were recorded, leading to early termination of the study. <br/><br>
<br/><br>
Conclusions:<br/><br>
Although this was an unsuccessful attempt to formulate an acrylate/methacrylate mix, our experience will be useful for those embarking on future attempts to do this.},
  author       = {Goon, Anthony and Bruze, Magnus and Zimerson, Erik and Goossens, An and Goh, Chee-Leok and Isaksson, Marléne},
  issn         = {0105-1873},
  language     = {eng},
  number       = {3},
  pages        = {157--161},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Contact Dermatitis},
  title        = {High frequency of false-positive reactions in attempted patch testing with acrylate/methacrylate mixes.},
  url          = {http://dx.doi.org/10.1111/j.1600-0536.2012.02106.x},
  volume       = {67},
  year         = {2012},
}