Advanced

Bile stimulates Cell Surface Hydrophobicity, Congo Red Binding and Biofilm Formation of Lactobacillus strains.

Ambalam, Padma; Kondepudi, Kanthi Kiran; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2012) In FEMS Microbiology Letters 333(1). p.10-19
Abstract
Seventeen Lactobacillus strains were tested for cell surface hydrophobicity (CSH) using the salt aggregation test (SAT) and Congo red binding (CRB) assay. CRB was pH and ionic strength dependent and protease sensitive and in the presence of 100 μg/ml cholesterol, the CRB was significantly reduced. Autoaggregating (AA) L. crispatus strains showed 50% more CRB than the reference strain, the curli-producing E.coli MC4100. CRB of L. crispatus 12005, L. paracasei F8, L. plantarum F44 and L. paracasei F19 was enhanced when grown in MRS broth with 0.5% taurocholic acid (TA) or 5% porcine bile (PB) (P<0.05). CSH was also enhanced for the non-AA strains, L. plantarum F44, L. paracasei F19 and L. rhamnosus GG when grown in MRS broth with 0.5% TA,... (More)
Seventeen Lactobacillus strains were tested for cell surface hydrophobicity (CSH) using the salt aggregation test (SAT) and Congo red binding (CRB) assay. CRB was pH and ionic strength dependent and protease sensitive and in the presence of 100 μg/ml cholesterol, the CRB was significantly reduced. Autoaggregating (AA) L. crispatus strains showed 50% more CRB than the reference strain, the curli-producing E.coli MC4100. CRB of L. crispatus 12005, L. paracasei F8, L. plantarum F44 and L. paracasei F19 was enhanced when grown in MRS broth with 0.5% taurocholic acid (TA) or 5% porcine bile (PB) (P<0.05). CSH was also enhanced for the non-AA strains, L. plantarum F44, L. paracasei F19 and L. rhamnosus GG when grown in MRS broth with 0.5% TA, 5% PB or 0.25% mucin with enhanced biofilm formation in MRS broth with bile (P<0.05). Two AA strains, L. crispatus 12005 and L. paracasei F8, developed biofilm independent of bile or mucin. In summary, under bile stressed growth conditions, early (24 h cultures) biofilm formation is associated with an increase in hydrophobic cell surface proteins and high CRB. Late mature (72 h culture) biofilm contained more carbohydrates as shown by crystal violet staining. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Microbiology Letters
volume
333
issue
1
pages
10 - 19
publisher
Elsevier
external identifiers
  • wos:000306273600002
  • pmid:22563647
  • scopus:84863826234
ISSN
1574-6968
DOI
10.1111/j.1574-6968.2012.02590.x
language
English
LU publication?
yes
id
37b0aace-c18d-4ddb-a544-cdbf255810ef (old id 2609058)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22563647?dopt=Abstract
date added to LUP
2012-06-03 12:55:57
date last changed
2017-11-05 04:09:37
@article{37b0aace-c18d-4ddb-a544-cdbf255810ef,
  abstract     = {Seventeen Lactobacillus strains were tested for cell surface hydrophobicity (CSH) using the salt aggregation test (SAT) and Congo red binding (CRB) assay. CRB was pH and ionic strength dependent and protease sensitive and in the presence of 100 μg/ml cholesterol, the CRB was significantly reduced. Autoaggregating (AA) L. crispatus strains showed 50% more CRB than the reference strain, the curli-producing E.coli MC4100. CRB of L. crispatus 12005, L. paracasei F8, L. plantarum F44 and L. paracasei F19 was enhanced when grown in MRS broth with 0.5% taurocholic acid (TA) or 5% porcine bile (PB) (P&lt;0.05). CSH was also enhanced for the non-AA strains, L. plantarum F44, L. paracasei F19 and L. rhamnosus GG when grown in MRS broth with 0.5% TA, 5% PB or 0.25% mucin with enhanced biofilm formation in MRS broth with bile (P&lt;0.05). Two AA strains, L. crispatus 12005 and L. paracasei F8, developed biofilm independent of bile or mucin. In summary, under bile stressed growth conditions, early (24 h cultures) biofilm formation is associated with an increase in hydrophobic cell surface proteins and high CRB. Late mature (72 h culture) biofilm contained more carbohydrates as shown by crystal violet staining. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.All rights reserved.},
  author       = {Ambalam, Padma and Kondepudi, Kanthi Kiran and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1574-6968},
  language     = {eng},
  number       = {1},
  pages        = {10--19},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Bile stimulates Cell Surface Hydrophobicity, Congo Red Binding and Biofilm Formation of Lactobacillus strains.},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2012.02590.x},
  volume       = {333},
  year         = {2012},
}