Diversification and selection mechanisms for the production of protein repertoires : Lessons from the immune system
(2000) In Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology 83(1-3). p.53-62- Abstract
The physiological mechanism for producing antigen-specific antibodies is based on a two-phase neo-Darwinian process: the first phase consists of diversity generation (formation of the repertoire), and the second phase is antigen-mediated selection. In this article, we consider how the natural immunoglobulin gene-diversification processes can be exploited both in vivo and in vitro in order to allow the generation of novel antibody (and heterologous protein) repertoires.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/260d0e80-e44c-4570-a8b1-c9702267e2ce
- author
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- keywords
- Antibody, Immunoglobulin, Rerarrangement, Somatic hypermutation, Transgenic mouse
- in
- Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
- volume
- 83
- issue
- 1-3
- pages
- 53 - 62
- publisher
- Humana Press
- external identifiers
-
- pmid:10826948
- scopus:0034042445
- ISSN
- 0273-2289
- DOI
- 10.1385/ABAB:83:1-3:53
- language
- English
- LU publication?
- no
- id
- 260d0e80-e44c-4570-a8b1-c9702267e2ce
- date added to LUP
- 2023-12-06 17:20:00
- date last changed
- 2024-01-04 15:43:49
@article{260d0e80-e44c-4570-a8b1-c9702267e2ce, abstract = {{<p>The physiological mechanism for producing antigen-specific antibodies is based on a two-phase neo-Darwinian process: the first phase consists of diversity generation (formation of the repertoire), and the second phase is antigen-mediated selection. In this article, we consider how the natural immunoglobulin gene-diversification processes can be exploited both in vivo and in vitro in order to allow the generation of novel antibody (and heterologous protein) repertoires.</p>}}, author = {{Neuberger, Michael S. and Sale, Julian E. and Cumbers, Sarah J. and Jolly, Chris J. and Bemark, Mats P. and Ehrenstein, Michael R. and Lanoue, Astrid and Brüggemann, Marianne and Batista, Facundo D. and Davies, Sarah L. and Williams, Gareth T.}}, issn = {{0273-2289}}, keywords = {{Antibody; Immunoglobulin; Rerarrangement; Somatic hypermutation; Transgenic mouse}}, language = {{eng}}, number = {{1-3}}, pages = {{53--62}}, publisher = {{Humana Press}}, series = {{Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology}}, title = {{Diversification and selection mechanisms for the production of protein repertoires : Lessons from the immune system}}, url = {{http://dx.doi.org/10.1385/ABAB:83:1-3:53}}, doi = {{10.1385/ABAB:83:1-3:53}}, volume = {{83}}, year = {{2000}}, }