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Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner

Rogers, SL; Wiedemann, U; Häcker, Udo LU ; Turck, C and Vale, RD (2004) In Current Biology 14(20). p.1827-1833
Abstract
Members of the Rho/Rac/Cdc42 superfamily of GTPases [1,2] and their upstream activators, guanine nucleotide exchange factors (GEFs) [3], have emerged as key regulators of actin and microtubule dynamics. In their GTP bound form, these proteins interact with downstream effector molecules that alter actin and microtubule behavior. During Drosophila embryogenesis, a Galpha subunit (Concertina) and a Rho-type guanine nucleotide exchange factor (DRhoGEF2) have been implicated in the dramatic epithelial-cell shape changes that occur during gastrulation [4-6] and morphogenesis [7]. Using Drosophila S2 cells as a model system, we show that DRhoGEF2 induces contractile cell shape changes by stimulating myosin II via the Rho1 pathway. Unexpectedly,... (More)
Members of the Rho/Rac/Cdc42 superfamily of GTPases [1,2] and their upstream activators, guanine nucleotide exchange factors (GEFs) [3], have emerged as key regulators of actin and microtubule dynamics. In their GTP bound form, these proteins interact with downstream effector molecules that alter actin and microtubule behavior. During Drosophila embryogenesis, a Galpha subunit (Concertina) and a Rho-type guanine nucleotide exchange factor (DRhoGEF2) have been implicated in the dramatic epithelial-cell shape changes that occur during gastrulation [4-6] and morphogenesis [7]. Using Drosophila S2 cells as a model system, we show that DRhoGEF2 induces contractile cell shape changes by stimulating myosin II via the Rho1 pathway. Unexpectedly, we found that DRhoGEF2 travels to the cell cortex on the tips of growing microtubules by interaction with the microtubule plus-end tracking protein EB1. The upstream activator Concertina, in its GTP but not GDP bound form, dissociates DRhoGEF2 from microtubule tips and also causes cellular contraction. We propose that DRhoGEF2 uses microtubule dynamics to search for cortical subdomains of receptor-mediated Galpha activation, which in turn causes localized actomyosin contraction associated with morphogenetic movements during development. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Biology
volume
14
issue
20
pages
1827 - 1833
publisher
Elsevier
external identifiers
  • pmid:15498490
  • wos:000224784200021
  • scopus:6944252346
ISSN
1879-0445
DOI
10.1016/j.cub.2004.09.078
language
English
LU publication?
yes
id
0b33487e-65bb-4e58-9edb-4ef2583351eb (old id 262498)
date added to LUP
2007-10-31 08:37:17
date last changed
2017-10-29 03:31:23
@article{0b33487e-65bb-4e58-9edb-4ef2583351eb,
  abstract     = {Members of the Rho/Rac/Cdc42 superfamily of GTPases [1,2] and their upstream activators, guanine nucleotide exchange factors (GEFs) [3], have emerged as key regulators of actin and microtubule dynamics. In their GTP bound form, these proteins interact with downstream effector molecules that alter actin and microtubule behavior. During Drosophila embryogenesis, a Galpha subunit (Concertina) and a Rho-type guanine nucleotide exchange factor (DRhoGEF2) have been implicated in the dramatic epithelial-cell shape changes that occur during gastrulation [4-6] and morphogenesis [7]. Using Drosophila S2 cells as a model system, we show that DRhoGEF2 induces contractile cell shape changes by stimulating myosin II via the Rho1 pathway. Unexpectedly, we found that DRhoGEF2 travels to the cell cortex on the tips of growing microtubules by interaction with the microtubule plus-end tracking protein EB1. The upstream activator Concertina, in its GTP but not GDP bound form, dissociates DRhoGEF2 from microtubule tips and also causes cellular contraction. We propose that DRhoGEF2 uses microtubule dynamics to search for cortical subdomains of receptor-mediated Galpha activation, which in turn causes localized actomyosin contraction associated with morphogenetic movements during development.},
  author       = {Rogers, SL and Wiedemann, U and Häcker, Udo and Turck, C and Vale, RD},
  issn         = {1879-0445},
  language     = {eng},
  number       = {20},
  pages        = {1827--1833},
  publisher    = {Elsevier},
  series       = {Current Biology},
  title        = {Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner},
  url          = {http://dx.doi.org/10.1016/j.cub.2004.09.078},
  volume       = {14},
  year         = {2004},
}