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Mucin expression in peripheral airways of patients with chronic obstructive pulmonary disease

Caramori, G; Di Gregorio, C; Carlstedt, Ingemar LU ; Casolari, P; Guzzinati, I; Adcock, IM; Barnes, PJ; Ciaccia, A; Cavallesco, G and Chung, KF, et al. (2004) In Histopathology 45(5). p.477-484
Abstract
Aims: To study the expression of mucins in peripheral airways in patients with chronic obstructive pulmonary disease (COPD). Methods and results: Peripheral lung sections from smokers with COPD (n = 9) and age-matched controls including smokers (n = 11) and lifelong non-smokers with normal lung function (n = 6) were stained with alcian blue, periodic acid-Schiff (PAS) and by immunohistochemistry of mucins (MUC): MUC2, MUC4, MUC5AC, MUC5B and MUC6. Histochemical staining and immunoreactivity of bronchiolar epithelium were graded and the presence or absence of stained mucus in the bronchiolar lumen was evaluated. There were no differences in alcian blue and PAS epithelial staining between the three groups. Intraluminal PAS staining was... (More)
Aims: To study the expression of mucins in peripheral airways in patients with chronic obstructive pulmonary disease (COPD). Methods and results: Peripheral lung sections from smokers with COPD (n = 9) and age-matched controls including smokers (n = 11) and lifelong non-smokers with normal lung function (n = 6) were stained with alcian blue, periodic acid-Schiff (PAS) and by immunohistochemistry of mucins (MUC): MUC2, MUC4, MUC5AC, MUC5B and MUC6. Histochemical staining and immunoreactivity of bronchiolar epithelium were graded and the presence or absence of stained mucus in the bronchiolar lumen was evaluated. There were no differences in alcian blue and PAS epithelial staining between the three groups. Intraluminal PAS staining was significantly more frequent among COPD subjects (P < 0.05). The expression of MUC5AC was significantly higher in the bronchiolar epithelium of patients with COPD (P < 0.05). Within the bronchiolar lumen, the predominant mucin was MUC5B. Intraluminal MUC5B was significantly more frequent among COPD patients (P < 0.05). Conclusions: COPD is specifically associated with increased expression of MUC5B in the bronchiolar lumen and of the mucin MUC5AC in the bronchiolar epithelium. These changes in mucin production in the peripheral airways may contribute to the pathophysiology of COPD. (Less)
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Contribution to journal
publication status
published
subject
keywords
MUC5B, COPD, mucins, MUC5AC, peripheral airways
in
Histopathology
volume
45
issue
5
pages
477 - 484
publisher
Wiley-Blackwell
external identifiers
  • wos:000224634200005
  • pmid:15500651
  • scopus:8744309317
ISSN
0309-0167
DOI
10.1111/j.1365-2559.2004.01952.x
language
English
LU publication?
yes
id
74d47cc6-edb1-4100-8832-9d7f25947d10 (old id 263733)
date added to LUP
2007-10-17 15:12:01
date last changed
2017-10-01 03:38:29
@article{74d47cc6-edb1-4100-8832-9d7f25947d10,
  abstract     = {Aims: To study the expression of mucins in peripheral airways in patients with chronic obstructive pulmonary disease (COPD). Methods and results: Peripheral lung sections from smokers with COPD (n = 9) and age-matched controls including smokers (n = 11) and lifelong non-smokers with normal lung function (n = 6) were stained with alcian blue, periodic acid-Schiff (PAS) and by immunohistochemistry of mucins (MUC): MUC2, MUC4, MUC5AC, MUC5B and MUC6. Histochemical staining and immunoreactivity of bronchiolar epithelium were graded and the presence or absence of stained mucus in the bronchiolar lumen was evaluated. There were no differences in alcian blue and PAS epithelial staining between the three groups. Intraluminal PAS staining was significantly more frequent among COPD subjects (P &lt; 0.05). The expression of MUC5AC was significantly higher in the bronchiolar epithelium of patients with COPD (P &lt; 0.05). Within the bronchiolar lumen, the predominant mucin was MUC5B. Intraluminal MUC5B was significantly more frequent among COPD patients (P &lt; 0.05). Conclusions: COPD is specifically associated with increased expression of MUC5B in the bronchiolar lumen and of the mucin MUC5AC in the bronchiolar epithelium. These changes in mucin production in the peripheral airways may contribute to the pathophysiology of COPD.},
  author       = {Caramori, G and Di Gregorio, C and Carlstedt, Ingemar and Casolari, P and Guzzinati, I and Adcock, IM and Barnes, PJ and Ciaccia, A and Cavallesco, G and Chung, KF and Papi, A},
  issn         = {0309-0167},
  keyword      = {MUC5B,COPD,mucins,MUC5AC,peripheral airways},
  language     = {eng},
  number       = {5},
  pages        = {477--484},
  publisher    = {Wiley-Blackwell},
  series       = {Histopathology},
  title        = {Mucin expression in peripheral airways of patients with chronic obstructive pulmonary disease},
  url          = {http://dx.doi.org/10.1111/j.1365-2559.2004.01952.x},
  volume       = {45},
  year         = {2004},
}