Development of Duffy transgenic mouse: in vivo expression of human Duffy gene with -33T -> C promoter mutation in non-erythroid tissues
(2004) In British Journal of Haematology 127(3). p.356-359- Abstract
- Blood group Duffy gene (FY) promoter in Duffy-negative individuals contains a point mutation in the GATA1 protein-binding motif, which was suggested to be responsible for erythroid suppression of FY. We developed two transgenic mouse lines with FY from both Duffy phenotypes. Transgenic mice with FY from Duffy-positive phenotype expressed Duffy protein both in red blood cells (RBCs) and non-erythroid tissues. Transgenic mice with FY from Duffy-negative phenotype did not express Duffy protein in RBCs, but it was expressed in non-erythroid tissues. This is the first in vivo experimental evidence showing the effect of -33T-->C promoter mutation on FY expression.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/264402
- author
- Chaudhuri, A ; Yuen, G ; Fang, F and Storry, Jill LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- erythroid-non-erythroid expression, transgenic mice, Duffy gene (FY), -33T -> C mutation
- in
- British Journal of Haematology
- volume
- 127
- issue
- 3
- pages
- 356 - 359
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:15491299
- wos:000224484800015
- scopus:7944222649
- pmid:15491299
- ISSN
- 0007-1048
- DOI
- 10.1111/j.1365-2141.2004.05208.x
- language
- English
- LU publication?
- yes
- id
- bc616219-54b8-4082-8cfc-8d4483f4bebe (old id 264402)
- date added to LUP
- 2016-04-01 11:49:14
- date last changed
- 2022-07-07 23:45:49
@article{bc616219-54b8-4082-8cfc-8d4483f4bebe, abstract = {{Blood group Duffy gene (FY) promoter in Duffy-negative individuals contains a point mutation in the GATA1 protein-binding motif, which was suggested to be responsible for erythroid suppression of FY. We developed two transgenic mouse lines with FY from both Duffy phenotypes. Transgenic mice with FY from Duffy-positive phenotype expressed Duffy protein both in red blood cells (RBCs) and non-erythroid tissues. Transgenic mice with FY from Duffy-negative phenotype did not express Duffy protein in RBCs, but it was expressed in non-erythroid tissues. This is the first in vivo experimental evidence showing the effect of -33T-->C promoter mutation on FY expression.}}, author = {{Chaudhuri, A and Yuen, G and Fang, F and Storry, Jill}}, issn = {{0007-1048}}, keywords = {{erythroid-non-erythroid expression; transgenic mice; Duffy gene (FY); -33T -> C mutation}}, language = {{eng}}, number = {{3}}, pages = {{356--359}}, publisher = {{Wiley-Blackwell}}, series = {{British Journal of Haematology}}, title = {{Development of Duffy transgenic mouse: in vivo expression of human Duffy gene with -33T -> C promoter mutation in non-erythroid tissues}}, url = {{http://dx.doi.org/10.1111/j.1365-2141.2004.05208.x}}, doi = {{10.1111/j.1365-2141.2004.05208.x}}, volume = {{127}}, year = {{2004}}, }