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Development of Duffy transgenic mouse: in vivo expression of human Duffy gene with -33T -> C promoter mutation in non-erythroid tissues

Chaudhuri, A; Yuen, G; Fang, F and Storry, Jill LU (2004) In British Journal of Haematology 127(3). p.356-359
Abstract
Blood group Duffy gene (FY) promoter in Duffy-negative individuals contains a point mutation in the GATA1 protein-binding motif, which was suggested to be responsible for erythroid suppression of FY. We developed two transgenic mouse lines with FY from both Duffy phenotypes. Transgenic mice with FY from Duffy-positive phenotype expressed Duffy protein both in red blood cells (RBCs) and non-erythroid tissues. Transgenic mice with FY from Duffy-negative phenotype did not express Duffy protein in RBCs, but it was expressed in non-erythroid tissues. This is the first in vivo experimental evidence showing the effect of -33T-->C promoter mutation on FY expression.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
erythroid-non-erythroid expression, transgenic mice, Duffy gene (FY), -33T -> C mutation
in
British Journal of Haematology
volume
127
issue
3
pages
356 - 359
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:15491299
  • wos:000224484800015
  • scopus:7944222649
ISSN
0007-1048
DOI
10.1111/j.1365-2141.2004.05208.x
language
English
LU publication?
yes
id
bc616219-54b8-4082-8cfc-8d4483f4bebe (old id 264402)
date added to LUP
2007-10-17 16:41:52
date last changed
2017-10-22 03:38:52
@article{bc616219-54b8-4082-8cfc-8d4483f4bebe,
  abstract     = {Blood group Duffy gene (FY) promoter in Duffy-negative individuals contains a point mutation in the GATA1 protein-binding motif, which was suggested to be responsible for erythroid suppression of FY. We developed two transgenic mouse lines with FY from both Duffy phenotypes. Transgenic mice with FY from Duffy-positive phenotype expressed Duffy protein both in red blood cells (RBCs) and non-erythroid tissues. Transgenic mice with FY from Duffy-negative phenotype did not express Duffy protein in RBCs, but it was expressed in non-erythroid tissues. This is the first in vivo experimental evidence showing the effect of -33T-->C promoter mutation on FY expression.},
  author       = {Chaudhuri, A and Yuen, G and Fang, F and Storry, Jill},
  issn         = {0007-1048},
  keyword      = {erythroid-non-erythroid expression,transgenic mice,Duffy gene (FY),-33T -> C mutation},
  language     = {eng},
  number       = {3},
  pages        = {356--359},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {British Journal of Haematology},
  title        = {Development of Duffy transgenic mouse: in vivo expression of human Duffy gene with -33T -> C promoter mutation in non-erythroid tissues},
  url          = {http://dx.doi.org/10.1111/j.1365-2141.2004.05208.x},
  volume       = {127},
  year         = {2004},
}