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Thiol metabolism in preterm infants during the first week of life

Ahola, T; Levonen, AL; Fellman, Vineta LU and Lapatto, R (2004) In Scandinavian Journal of Clinical & Laboratory Investigation 64(7). p.649-658
Abstract
Background: Oxidative stress is implicated in the pathogenesis of several complications of prematurity. The glutathione cycle is one of the most important intracellular antioxidant systems. The synthesis of glutathione may not be adequate in preterm neonates because of the low levels of cysteine available. The aim of this study was to evaluate cysteine and glutathione metabolism during the first week of life in preterm infants. Methods: Plasma and erythrocyte thiol concentrations were measured in 78 preterm infants with a birthweight of 500 1500 g, and erythrocyte glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferases (GST) and glucose 6-phosphatedehydrogenase (G6PDH) in 26... (More)
Background: Oxidative stress is implicated in the pathogenesis of several complications of prematurity. The glutathione cycle is one of the most important intracellular antioxidant systems. The synthesis of glutathione may not be adequate in preterm neonates because of the low levels of cysteine available. The aim of this study was to evaluate cysteine and glutathione metabolism during the first week of life in preterm infants. Methods: Plasma and erythrocyte thiol concentrations were measured in 78 preterm infants with a birthweight of 500 1500 g, and erythrocyte glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferases (GST) and glucose 6-phosphatedehydrogenase (G6PDH) in 26 infants with a birthweight of 1000 - 1500 g. Results: The mean (SD) plasma glutathione concentration increased from day 0 to day 1 (14.9 (7.1) vs. 27.7 (11.9) mumol/L, p<0.001), and then decreased. The plasma cysteine concentration changed in the opposite direction ( 172 (59) vs. 129 (42) μmol/L, p<0.01). In infants with respiratory distress syndrome (RDS) the mean plasma glutathione concentration, but not cysteine, was lower on day 0 compared with infants without RDS (11.7 (5.2) vs. 21.4 (5.6) mumol/L, p<0.01). Erythrocyte glutathione concentration decreased during the first week of life, whereas erythrocyte cysteine concentration increased significantly from day 3 to day 7 (p<0.01). Erythrocyte cysteine and glutathione concentrations had a positive correlation. The GCL and GR activities did not change, but GST and G6PDH activities decreased during the first week (p<0.01). GPx activity decreased until day 3 (p<0.01) and was higher on day 0 and day 1 in infants with RDS. Conclusions: Very low birthweight infants have an initial increase in plasma glutathione and initial decrease in plasma cysteine level during the first week of life, and also a positive correlation between erythrocyte cysteine and glutathione levels. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glutathione peroxidase, glutathione, glutamate-cysteine ligase, glucose 6-phosphatedehydrogenase, bronchopulmonary dysplasia, cysteine, glutathione reductase, glutathione transferase, respiratory distress, syndrome
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
64
issue
7
pages
649 - 658
publisher
Informa Healthcare
external identifiers
  • pmid:15513322
  • wos:000224328400006
  • scopus:7044222381
ISSN
1502-7686
DOI
10.1080/00365510410002959
language
English
LU publication?
yes
id
7cb1bb8d-1f32-467f-b531-70ac7b8b9411 (old id 264441)
date added to LUP
2007-08-28 16:33:43
date last changed
2017-05-21 04:21:32
@article{7cb1bb8d-1f32-467f-b531-70ac7b8b9411,
  abstract     = {Background: Oxidative stress is implicated in the pathogenesis of several complications of prematurity. The glutathione cycle is one of the most important intracellular antioxidant systems. The synthesis of glutathione may not be adequate in preterm neonates because of the low levels of cysteine available. The aim of this study was to evaluate cysteine and glutathione metabolism during the first week of life in preterm infants. Methods: Plasma and erythrocyte thiol concentrations were measured in 78 preterm infants with a birthweight of 500 1500 g, and erythrocyte glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferases (GST) and glucose 6-phosphatedehydrogenase (G6PDH) in 26 infants with a birthweight of 1000 - 1500 g. Results: The mean (SD) plasma glutathione concentration increased from day 0 to day 1 (14.9 (7.1) vs. 27.7 (11.9) mumol/L, p&lt;0.001), and then decreased. The plasma cysteine concentration changed in the opposite direction ( 172 (59) vs. 129 (42) μmol/L, p&lt;0.01). In infants with respiratory distress syndrome (RDS) the mean plasma glutathione concentration, but not cysteine, was lower on day 0 compared with infants without RDS (11.7 (5.2) vs. 21.4 (5.6) mumol/L, p&lt;0.01). Erythrocyte glutathione concentration decreased during the first week of life, whereas erythrocyte cysteine concentration increased significantly from day 3 to day 7 (p&lt;0.01). Erythrocyte cysteine and glutathione concentrations had a positive correlation. The GCL and GR activities did not change, but GST and G6PDH activities decreased during the first week (p&lt;0.01). GPx activity decreased until day 3 (p&lt;0.01) and was higher on day 0 and day 1 in infants with RDS. Conclusions: Very low birthweight infants have an initial increase in plasma glutathione and initial decrease in plasma cysteine level during the first week of life, and also a positive correlation between erythrocyte cysteine and glutathione levels.},
  author       = {Ahola, T and Levonen, AL and Fellman, Vineta and Lapatto, R},
  issn         = {1502-7686},
  keyword      = {glutathione peroxidase,glutathione,glutamate-cysteine ligase,glucose 6-phosphatedehydrogenase,bronchopulmonary dysplasia,cysteine,glutathione reductase,glutathione transferase,respiratory distress,syndrome},
  language     = {eng},
  number       = {7},
  pages        = {649--658},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Thiol metabolism in preterm infants during the first week of life},
  url          = {http://dx.doi.org/10.1080/00365510410002959},
  volume       = {64},
  year         = {2004},
}