Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Sex steroid levels in corresponding cerebrospinal fluid and serum samples quantified by mass spectrometry in men

Ryberg, Henrik ; Norlén, Anna-Karin ; Landin, Andreas ; Johansson, Per LU ; Salman, Zeinab ; Wallin, Anders ; Svensson, Johan and Ohlsson, Claes (2023) In Endocrine Connections 13(1).
Abstract

OBJECTIVE: Sex steroids exert important biological functions within the central nervous system (CNS), but the underlying mechanisms are poorly understood. The contribution of circulating sex steroids to the levels in CNS tissue and cerebrospinal fluid (CSF) has been sparsely investigated in human and with inconclusive results. This could partly be due to lack of sensitive validated assays. To address this, we validated a gas chromatographic-mass spectrometric (GC-MS/MS) assay for quantification of sex steroid hormones/precursors in CSF.

METHODS: GC-MS/MS quantification of dihydrotestosterone (DHT, CSF lower limit of quantification, 1.5 pg/mL), testosterone (T, 4.9), estrone (E1, 0.88), estradiol (E2, 0.25), dehydroepiandrosterone... (More)

OBJECTIVE: Sex steroids exert important biological functions within the central nervous system (CNS), but the underlying mechanisms are poorly understood. The contribution of circulating sex steroids to the levels in CNS tissue and cerebrospinal fluid (CSF) has been sparsely investigated in human and with inconclusive results. This could partly be due to lack of sensitive validated assays. To address this, we validated a gas chromatographic-mass spectrometric (GC-MS/MS) assay for quantification of sex steroid hormones/precursors in CSF.

METHODS: GC-MS/MS quantification of dihydrotestosterone (DHT, CSF lower limit of quantification, 1.5 pg/mL), testosterone (T, 4.9), estrone (E1, 0.88), estradiol (E2, 0.25), dehydroepiandrosterone (DHEA, 38.4), androstenedione (4D, 22.3) and progesterone (P, 4.2) in CSF, and corresponding serum samples from 47 men.

RESULTS: Analyses of CSF revealed that DHEA was the major sex steroid (73.5±31.7 pg/mL) followed by 4D (61.4±29.6 pg/mL) and T (49.5±18.9 pg/mL). The CSF levels of DHT, E2 and E1 were substantially lower, and P was in general not detectable in CSF. For all sex steroids except E2, strong associations between corresponding CSF and serum levels were observed. We propose that T in CSF is derived from circulating T, DHT in CSF is from local conversion from T, while E2 in CSF is from local conversion from 4D in CNS.

CONCLUSIONS: We describe the first thoroughly validated high-sensitive mass spectrometric assay for a broad sex steroid hormone panel suitable for human CSF. This assay constitutes a new tool for investigation of the role of sex steroid hormones in the human CNS.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Endocrine Connections
volume
13
issue
1
publisher
BioScientifica
external identifiers
  • scopus:85180439964
  • pmid:37966483
ISSN
2049-3614
DOI
10.1530/EC-23-0250
language
English
LU publication?
no
id
2652ceb5-a97a-4161-b95f-9c9b63b0f13b
date added to LUP
2023-11-29 14:57:34
date last changed
2024-05-29 10:21:42
@article{2652ceb5-a97a-4161-b95f-9c9b63b0f13b,
  abstract     = {{<p>OBJECTIVE: Sex steroids exert important biological functions within the central nervous system (CNS), but the underlying mechanisms are poorly understood. The contribution of circulating sex steroids to the levels in CNS tissue and cerebrospinal fluid (CSF) has been sparsely investigated in human and with inconclusive results. This could partly be due to lack of sensitive validated assays. To address this, we validated a gas chromatographic-mass spectrometric (GC-MS/MS) assay for quantification of sex steroid hormones/precursors in CSF.</p><p>METHODS: GC-MS/MS quantification of dihydrotestosterone (DHT, CSF lower limit of quantification, 1.5 pg/mL), testosterone (T, 4.9), estrone (E1, 0.88), estradiol (E2, 0.25), dehydroepiandrosterone (DHEA, 38.4), androstenedione (4D, 22.3) and progesterone (P, 4.2) in CSF, and corresponding serum samples from 47 men.</p><p>RESULTS: Analyses of CSF revealed that DHEA was the major sex steroid (73.5±31.7 pg/mL) followed by 4D (61.4±29.6 pg/mL) and T (49.5±18.9 pg/mL). The CSF levels of DHT, E2 and E1 were substantially lower, and P was in general not detectable in CSF. For all sex steroids except E2, strong associations between corresponding CSF and serum levels were observed. We propose that T in CSF is derived from circulating T, DHT in CSF is from local conversion from T, while E2 in CSF is from local conversion from 4D in CNS.</p><p>CONCLUSIONS: We describe the first thoroughly validated high-sensitive mass spectrometric assay for a broad sex steroid hormone panel suitable for human CSF. This assay constitutes a new tool for investigation of the role of sex steroid hormones in the human CNS.</p>}},
  author       = {{Ryberg, Henrik and Norlén, Anna-Karin and Landin, Andreas and Johansson, Per and Salman, Zeinab and Wallin, Anders and Svensson, Johan and Ohlsson, Claes}},
  issn         = {{2049-3614}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{1}},
  publisher    = {{BioScientifica}},
  series       = {{Endocrine Connections}},
  title        = {{Sex steroid levels in corresponding cerebrospinal fluid and serum samples quantified by mass spectrometry in men}},
  url          = {{http://dx.doi.org/10.1530/EC-23-0250}},
  doi          = {{10.1530/EC-23-0250}},
  volume       = {{13}},
  year         = {{2023}},
}