Sex steroid levels in corresponding cerebrospinal fluid and serum samples quantified by mass spectrometry in men
(2023) In Endocrine Connections 13(1).- Abstract
OBJECTIVE: Sex steroids exert important biological functions within the central nervous system (CNS), but the underlying mechanisms are poorly understood. The contribution of circulating sex steroids to the levels in CNS tissue and cerebrospinal fluid (CSF) has been sparsely investigated in human and with inconclusive results. This could partly be due to lack of sensitive validated assays. To address this, we validated a gas chromatographic-mass spectrometric (GC-MS/MS) assay for quantification of sex steroid hormones/precursors in CSF.
METHODS: GC-MS/MS quantification of dihydrotestosterone (DHT, CSF lower limit of quantification, 1.5 pg/mL), testosterone (T, 4.9), estrone (E1, 0.88), estradiol (E2, 0.25), dehydroepiandrosterone... (More)
OBJECTIVE: Sex steroids exert important biological functions within the central nervous system (CNS), but the underlying mechanisms are poorly understood. The contribution of circulating sex steroids to the levels in CNS tissue and cerebrospinal fluid (CSF) has been sparsely investigated in human and with inconclusive results. This could partly be due to lack of sensitive validated assays. To address this, we validated a gas chromatographic-mass spectrometric (GC-MS/MS) assay for quantification of sex steroid hormones/precursors in CSF.
METHODS: GC-MS/MS quantification of dihydrotestosterone (DHT, CSF lower limit of quantification, 1.5 pg/mL), testosterone (T, 4.9), estrone (E1, 0.88), estradiol (E2, 0.25), dehydroepiandrosterone (DHEA, 38.4), androstenedione (4D, 22.3) and progesterone (P, 4.2) in CSF, and corresponding serum samples from 47 men.
RESULTS: Analyses of CSF revealed that DHEA was the major sex steroid (73.5±31.7 pg/mL) followed by 4D (61.4±29.6 pg/mL) and T (49.5±18.9 pg/mL). The CSF levels of DHT, E2 and E1 were substantially lower, and P was in general not detectable in CSF. For all sex steroids except E2, strong associations between corresponding CSF and serum levels were observed. We propose that T in CSF is derived from circulating T, DHT in CSF is from local conversion from T, while E2 in CSF is from local conversion from 4D in CNS.
CONCLUSIONS: We describe the first thoroughly validated high-sensitive mass spectrometric assay for a broad sex steroid hormone panel suitable for human CSF. This assay constitutes a new tool for investigation of the role of sex steroid hormones in the human CNS.
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- author
- Ryberg, Henrik ; Norlén, Anna-Karin ; Landin, Andreas ; Johansson, Per LU ; Salman, Zeinab ; Wallin, Anders ; Svensson, Johan and Ohlsson, Claes
- publishing date
- 2023-11-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Endocrine Connections
- volume
- 13
- issue
- 1
- publisher
- BioScientifica
- external identifiers
-
- scopus:85180439964
- pmid:37966483
- ISSN
- 2049-3614
- DOI
- 10.1530/EC-23-0250
- language
- English
- LU publication?
- no
- id
- 2652ceb5-a97a-4161-b95f-9c9b63b0f13b
- date added to LUP
- 2023-11-29 14:57:34
- date last changed
- 2024-04-17 06:06:16
@article{2652ceb5-a97a-4161-b95f-9c9b63b0f13b,
abstract = {{<p>OBJECTIVE: Sex steroids exert important biological functions within the central nervous system (CNS), but the underlying mechanisms are poorly understood. The contribution of circulating sex steroids to the levels in CNS tissue and cerebrospinal fluid (CSF) has been sparsely investigated in human and with inconclusive results. This could partly be due to lack of sensitive validated assays. To address this, we validated a gas chromatographic-mass spectrometric (GC-MS/MS) assay for quantification of sex steroid hormones/precursors in CSF.</p><p>METHODS: GC-MS/MS quantification of dihydrotestosterone (DHT, CSF lower limit of quantification, 1.5 pg/mL), testosterone (T, 4.9), estrone (E1, 0.88), estradiol (E2, 0.25), dehydroepiandrosterone (DHEA, 38.4), androstenedione (4D, 22.3) and progesterone (P, 4.2) in CSF, and corresponding serum samples from 47 men.</p><p>RESULTS: Analyses of CSF revealed that DHEA was the major sex steroid (73.5±31.7 pg/mL) followed by 4D (61.4±29.6 pg/mL) and T (49.5±18.9 pg/mL). The CSF levels of DHT, E2 and E1 were substantially lower, and P was in general not detectable in CSF. For all sex steroids except E2, strong associations between corresponding CSF and serum levels were observed. We propose that T in CSF is derived from circulating T, DHT in CSF is from local conversion from T, while E2 in CSF is from local conversion from 4D in CNS.</p><p>CONCLUSIONS: We describe the first thoroughly validated high-sensitive mass spectrometric assay for a broad sex steroid hormone panel suitable for human CSF. This assay constitutes a new tool for investigation of the role of sex steroid hormones in the human CNS.</p>}},
author = {{Ryberg, Henrik and Norlén, Anna-Karin and Landin, Andreas and Johansson, Per and Salman, Zeinab and Wallin, Anders and Svensson, Johan and Ohlsson, Claes}},
issn = {{2049-3614}},
language = {{eng}},
month = {{11}},
number = {{1}},
publisher = {{BioScientifica}},
series = {{Endocrine Connections}},
title = {{Sex steroid levels in corresponding cerebrospinal fluid and serum samples quantified by mass spectrometry in men}},
url = {{http://dx.doi.org/10.1530/EC-23-0250}},
doi = {{10.1530/EC-23-0250}},
volume = {{13}},
year = {{2023}},
}