Specific pancreatic β-cell surface antigens recognized by a xenogenic antiserum
Dyrberg, Thomas ; Baekkeskov, Steinunn and Lernmark, Åke LU
(1982)
In Journal of Cell Biology
94(2).
p.472-477
- Abstract
An antiserum (R4) from a rabbit immunized with suspensions of C57BL/6J ob/ob mouse islet cells contains antibodies which in a 125I-protein A radioligand assay can be demonstrated to bind to single cell suspensions of normal Naval Medical Research Institute (NMRI) mouse islet cells. The binding of 125I-protein A to islet cells was about four times that of normal rabbit serum (NRS) after incubation at a 1/600 dilution of R4 antiserum quantitatively absorbed to mouse spleen lymphocytes (R4A antiserum) and hepatocytes. Subsequent absorption of the R4A antiserum to islet cells significantly reduced the binding of 125I-protein A to islet cells incubated with the doubly absorbed serum. Immunoprecipitation of... (More)
An antiserum (R4) from a rabbit immunized with suspensions of C57BL/6J ob/ob mouse islet cells contains antibodies which in a 125I-protein A radioligand assay can be demonstrated to bind to single cell suspensions of normal Naval Medical Research Institute (NMRI) mouse islet cells. The binding of 125I-protein A to islet cells was about four times that of normal rabbit serum (NRS) after incubation at a 1/600 dilution of R4 antiserum quantitatively absorbed to mouse spleen lymphocytes (R4A antiserum) and hepatocytes. Subsequent absorption of the R4A antiserum to islet cells significantly reduced the binding of 125I-protein A to islet cells incubated with the doubly absorbed serum. Immunoprecipitation of radiolabeled islet cell lysates followed by SDS polyacrylamide gel electrophoresis and autoradiography suggested that the R4A antiserum recognized a Mr 40,000 glycoprotein. This glycoprotein was not detected in spleen lymphocytes. Electron microscope detection of gold-protein A complexes suggested that the binding of islet cell surface antibodies was cell specific. Islet cell suspensions incubated with R4A antiserum and gold-protein A showed that 86 ± 3 gold particles were bound per 100 β-cells (mean ± SE for six experiments). In contrast, the number of gold particles per 100 endocrine non-β-cells was 8 ± 1 which was similar to the number achieved with NRS (3 ± 1) on all endocrine islet cells. Our observations suggest that the pancreatic islet cells, in particular the β-cells, express a specific antigen.
(Less)
- author
- Dyrberg, Thomas
; Baekkeskov, Steinunn
and Lernmark, Åke
LU
- publishing date
- 1982-08-01
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Cell Biology
- volume
- 94
- issue
- 2
- pages
- 6 pages
- publisher
- Rockefeller University Press
- external identifiers
-
- scopus:0020318925
- pmid:7050130
- ISSN
- 0021-9525
- DOI
- 10.1083/jcb.94.2.472
- language
- English
- LU publication?
- no
- id
- 26a27034-c0d6-49d7-af9d-ea0a4ed13af5
- date added to LUP
- 2019-09-16 15:28:08
- date last changed
- 2024-03-13 07:59:03
@article{26a27034-c0d6-49d7-af9d-ea0a4ed13af5,
abstract = {{<p>An antiserum (R4) from a rabbit immunized with suspensions of C57BL/6J ob/ob mouse islet cells contains antibodies which in a <sup>125</sup>I-protein A radioligand assay can be demonstrated to bind to single cell suspensions of normal Naval Medical Research Institute (NMRI) mouse islet cells. The binding of <sup>125</sup>I-protein A to islet cells was about four times that of normal rabbit serum (NRS) after incubation at a 1/600 dilution of R4 antiserum quantitatively absorbed to mouse spleen lymphocytes (R4A antiserum) and hepatocytes. Subsequent absorption of the R4A antiserum to islet cells significantly reduced the binding of <sup>125</sup>I-protein A to islet cells incubated with the doubly absorbed serum. Immunoprecipitation of radiolabeled islet cell lysates followed by SDS polyacrylamide gel electrophoresis and autoradiography suggested that the R4A antiserum recognized a M<sub>r</sub> 40,000 glycoprotein. This glycoprotein was not detected in spleen lymphocytes. Electron microscope detection of gold-protein A complexes suggested that the binding of islet cell surface antibodies was cell specific. Islet cell suspensions incubated with R4A antiserum and gold-protein A showed that 86 ± 3 gold particles were bound per 100 β-cells (mean ± SE for six experiments). In contrast, the number of gold particles per 100 endocrine non-β-cells was 8 ± 1 which was similar to the number achieved with NRS (3 ± 1) on all endocrine islet cells. Our observations suggest that the pancreatic islet cells, in particular the β-cells, express a specific antigen.</p>}},
author = {{Dyrberg, Thomas and Baekkeskov, Steinunn and Lernmark, Åke}},
issn = {{0021-9525}},
language = {{eng}},
month = {{08}},
number = {{2}},
pages = {{472--477}},
publisher = {{Rockefeller University Press}},
series = {{Journal of Cell Biology}},
title = {{Specific pancreatic β-cell surface antigens recognized by a xenogenic antiserum}},
url = {{http://dx.doi.org/10.1083/jcb.94.2.472}},
doi = {{10.1083/jcb.94.2.472}},
volume = {{94}},
year = {{1982}},
}