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The dynamics of genomic-length DNA molecules in 100-nm channels

Tegenfeldt, Jonas LU ; Prinz, Christelle LU ; Cao, H; Chou, S; Reisner, WW; Riehn, R; Wang, YM; Cox, EC; Sturm, JC and Silberzan, P, et al. (2004) In Proceedings of the National Academy of Sciences 101(30). p.10979-10983
Abstract
We show that genomic-length DNA molecules imaged in nano-channels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of A DNA multimers with a 130-nm SD in 1 min.
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Proceedings of the National Academy of Sciences
volume
101
issue
30
pages
10979 - 10983
publisher
National Acad Sciences
external identifiers
  • wos:000223000200026
  • scopus:4043068454
ISSN
1091-6490
DOI
10.1073/pnas.0403849101
language
English
LU publication?
yes
id
c99c04f2-fc6a-464b-868c-23c0c844993a (old id 271610)
date added to LUP
2007-10-26 12:14:55
date last changed
2017-12-10 03:44:59
@article{c99c04f2-fc6a-464b-868c-23c0c844993a,
  abstract     = {We show that genomic-length DNA molecules imaged in nano-channels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of A DNA multimers with a 130-nm SD in 1 min.},
  author       = {Tegenfeldt, Jonas and Prinz, Christelle and Cao, H and Chou, S and Reisner, WW and Riehn, R and Wang, YM and Cox, EC and Sturm, JC and Silberzan, P and Austin, RH},
  issn         = {1091-6490},
  language     = {eng},
  number       = {30},
  pages        = {10979--10983},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {The dynamics of genomic-length DNA molecules in 100-nm channels},
  url          = {http://dx.doi.org/10.1073/pnas.0403849101},
  volume       = {101},
  year         = {2004},
}