Iron-chelated thermoresponsive polymer brushes on bismuth titanate nanosheets for metal affinity separation of phosphoproteins
Guo, Peng Fei LU ; Gong, Hai Yue LU ; Zheng, Hong Wei LU ; Chen, Ming Li ; Wang, Jian Hua and Ye, Lei LU
(2020)
In Colloids and Surfaces B: Biointerfaces
196.
- Abstract
Separation of phosphoproteins plays an important role for identification of biomarkers in life science. In this work, bismuth titanate supported, iron-chelated thermoresponsive polymer brushes were prepared for selective separation of phosphoproteins. The iron-chelated thermoresponsive polymer brushes were synthesized by surface-initiated atom transfer radical polymerization of N-isopropylacrylamide and glycidyl methacrylate, followed by a ring opening reaction of epoxy group, and chelation of the obtained cis-diols with Fe3+ ions. The composite material was characterized to determine the size and thickness, the content of the organic polymer and the metal loading. The bismuth titanate supported, iron-chelated... (More)
Separation of phosphoproteins plays an important role for identification of biomarkers in life science. In this work, bismuth titanate supported, iron-chelated thermoresponsive polymer brushes were prepared for selective separation of phosphoproteins. The iron-chelated thermoresponsive polymer brushes were synthesized by surface-initiated atom transfer radical polymerization of N-isopropylacrylamide and glycidyl methacrylate, followed by a ring opening reaction of epoxy group, and chelation of the obtained cis-diols with Fe3+ ions. The composite material was characterized to determine the size and thickness, the content of the organic polymer and the metal loading. The bismuth titanate supported, iron-chelated thermoresponsive polymer brushes showed selective binding for phosphoproteins in the presence of abundant interfering proteins, and a high binding capacity for phosphoproteins by virtue of the metal affinity between the metal ions on the polymer brushes and the phosphate groups in the phosphoproteins (664 mg β-Casein per g sorbent). The thermoresponsive property of the polymer brushes made it possible to adjust phosphoprotein binding by changing temperature. Finally, separation of phosphoproteins from a complex biological sample (i.e. milk) was demonstrated using the nanosheet-supported thermoresponsive polymer brushes.
(Less)
- author
- Guo, Peng Fei
LU
; Gong, Hai Yue
LU
; Zheng, Hong Wei
LU
; Chen, Ming Li
; Wang, Jian Hua
and Ye, Lei
LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bismuth titanate nanosheets, Copolymer brushes, Inorganic-organic hybrid, Metal affinity, Phosphoproteins, Thermoresponsive polymer
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 196
- article number
- 111282
- publisher
- Elsevier
- external identifiers
-
- scopus:85088856146
- pmid:32763792
- ISSN
- 0927-7765
- DOI
- 10.1016/j.colsurfb.2020.111282
- language
- English
- LU publication?
- yes
- id
- 2727a35a-a30f-437f-b070-b3c58f92ec96
- date added to LUP
- 2020-08-07 10:49:25
- date last changed
- 2024-05-15 16:57:37
@article{2727a35a-a30f-437f-b070-b3c58f92ec96,
abstract = {{<p>Separation of phosphoproteins plays an important role for identification of biomarkers in life science. In this work, bismuth titanate supported, iron-chelated thermoresponsive polymer brushes were prepared for selective separation of phosphoproteins. The iron-chelated thermoresponsive polymer brushes were synthesized by surface-initiated atom transfer radical polymerization of N-isopropylacrylamide and glycidyl methacrylate, followed by a ring opening reaction of epoxy group, and chelation of the obtained cis-diols with Fe<sup>3+</sup> ions. The composite material was characterized to determine the size and thickness, the content of the organic polymer and the metal loading. The bismuth titanate supported, iron-chelated thermoresponsive polymer brushes showed selective binding for phosphoproteins in the presence of abundant interfering proteins, and a high binding capacity for phosphoproteins by virtue of the metal affinity between the metal ions on the polymer brushes and the phosphate groups in the phosphoproteins (664 mg β-Casein per g sorbent). The thermoresponsive property of the polymer brushes made it possible to adjust phosphoprotein binding by changing temperature. Finally, separation of phosphoproteins from a complex biological sample (i.e. milk) was demonstrated using the nanosheet-supported thermoresponsive polymer brushes.</p>}},
author = {{Guo, Peng Fei and Gong, Hai Yue and Zheng, Hong Wei and Chen, Ming Li and Wang, Jian Hua and Ye, Lei}},
issn = {{0927-7765}},
keywords = {{Bismuth titanate nanosheets; Copolymer brushes; Inorganic-organic hybrid; Metal affinity; Phosphoproteins; Thermoresponsive polymer}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Iron-chelated thermoresponsive polymer brushes on bismuth titanate nanosheets for metal affinity separation of phosphoproteins}},
url = {{http://dx.doi.org/10.1016/j.colsurfb.2020.111282}},
doi = {{10.1016/j.colsurfb.2020.111282}},
volume = {{196}},
year = {{2020}},
}