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New antimicrobial peptide active against Gram-positive pathogens

Jasir, Aftab LU ; Kasprzykowski, F; Lindström, Veronica LU ; Schalén, Claës LU and Grubb, Anders LU (2004) In Indian Journal of Medical Research 119(Suppl.). p.74-76
Abstract
Background & objectives: Human and animal cystatins have been shown to inhibit the replication of certain viruses and bacteria, though it is not directly demonstrated that the effects are due to protease inhibitory capacity of the cystatins. We report antibacterial properties of a novel antimicrobial peptidyl derivative, (2S)-2-(N-alpha-benzyloxycarbonyl-arginyl-leucylamido)-1-(E)-cinnamoyla mido-3-methylbutane, structurally based upon the aminoterminal segment of the inhibitory centre of the human cysteine protease inhibitor, cystatin C. Methods: Clinical isolates of group A, B, C and G streptococci were collected. The antibacterial activity of Cystapep 1 derivative was tested by agar well diffusion method. Results: Cystapep 1,... (More)
Background & objectives: Human and animal cystatins have been shown to inhibit the replication of certain viruses and bacteria, though it is not directly demonstrated that the effects are due to protease inhibitory capacity of the cystatins. We report antibacterial properties of a novel antimicrobial peptidyl derivative, (2S)-2-(N-alpha-benzyloxycarbonyl-arginyl-leucylamido)-1-(E)-cinnamoyla mido-3-methylbutane, structurally based upon the aminoterminal segment of the inhibitory centre of the human cysteine protease inhibitor, cystatin C. Methods: Clinical isolates of group A, B, C and G streptococci were collected. The antibacterial activity of Cystapep 1 derivative was tested by agar well diffusion method. Results: Cystapep 1, displayed antibacterial activity against several clinically important Gram-positive bacteria. It displayed minimal inhibitory and bactericidal concentrations of about 16 mug/ml for both Staphylococcus aureus and Streptococcus pyogenes. In radial agar diffusion assays, groups A, B, C and G streptococci as well as staphylococci were generally susceptible to the action of Cystapep 1, whereas pneumococci and enterococci were less susceptible. No activity against Gram-negative bacteria was observed. Interpretation & conclusion: Cystapep 1 also showed high activity against methicillin-resistant Staph. aureus (MRSA) and multi-antibiotic resistant coagulase negative staphylococci (CNS), suggesting its mechanism of action to be different from most currently used antibiotics. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Staphylococcus aureus, antimicrobial peptide, cystatin C, Streptococcus, pyogenes
in
Indian Journal of Medical Research
volume
119
issue
Suppl.
pages
74 - 76
publisher
Council Of Medical Research
external identifiers
  • pmid:15232166
  • wos:000222679400017
  • scopus:3442877866
ISSN
0971-5916
language
English
LU publication?
yes
id
dfdc6eac-1d6e-4714-9481-1fd2176fc3d0 (old id 272832)
alternative location
http://www.icmr.nic.in/ijmr/ijmr_supp/15.pdf
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15232166&dopt=Abstract
date added to LUP
2007-10-23 11:19:56
date last changed
2017-07-23 04:47:29
@article{dfdc6eac-1d6e-4714-9481-1fd2176fc3d0,
  abstract     = {Background & objectives: Human and animal cystatins have been shown to inhibit the replication of certain viruses and bacteria, though it is not directly demonstrated that the effects are due to protease inhibitory capacity of the cystatins. We report antibacterial properties of a novel antimicrobial peptidyl derivative, (2S)-2-(N-alpha-benzyloxycarbonyl-arginyl-leucylamido)-1-(E)-cinnamoyla mido-3-methylbutane, structurally based upon the aminoterminal segment of the inhibitory centre of the human cysteine protease inhibitor, cystatin C. Methods: Clinical isolates of group A, B, C and G streptococci were collected. The antibacterial activity of Cystapep 1 derivative was tested by agar well diffusion method. Results: Cystapep 1, displayed antibacterial activity against several clinically important Gram-positive bacteria. It displayed minimal inhibitory and bactericidal concentrations of about 16 mug/ml for both Staphylococcus aureus and Streptococcus pyogenes. In radial agar diffusion assays, groups A, B, C and G streptococci as well as staphylococci were generally susceptible to the action of Cystapep 1, whereas pneumococci and enterococci were less susceptible. No activity against Gram-negative bacteria was observed. Interpretation & conclusion: Cystapep 1 also showed high activity against methicillin-resistant Staph. aureus (MRSA) and multi-antibiotic resistant coagulase negative staphylococci (CNS), suggesting its mechanism of action to be different from most currently used antibiotics.},
  author       = {Jasir, Aftab and Kasprzykowski, F and Lindström, Veronica and Schalén, Claës and Grubb, Anders},
  issn         = {0971-5916},
  keyword      = {Staphylococcus aureus,antimicrobial peptide,cystatin C,Streptococcus,pyogenes},
  language     = {eng},
  number       = {Suppl.},
  pages        = {74--76},
  publisher    = {Council Of Medical Research},
  series       = {Indian Journal of Medical Research},
  title        = {New antimicrobial peptide active against Gram-positive pathogens},
  volume       = {119},
  year         = {2004},
}