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Amplification of the cyclin D1 gene is associated with tumour subsite, DNA non-diploidy and high S-phase fraction in squamous cell carcinoma of the head and neck

Niméus, Emma LU ; Baldetorp, Bo LU ; Bendahl, Pär-Ola LU ; Rennstam, Karin LU ; Wennerberg, Johan LU ; Åkervall, Jan LU and Fernö, Mårten LU (2004) In Oral Oncology 40(6). p.624-629
Abstract
Amplification of CCND1 (cyclin D1 gene) in squamous cell carcinoma of the head and neck (SCCHN) is correlated to poor prognosis. The purpose of this study was to investigate whether CCND1 amplification is related to different subsites and also to DNA ploidy status and S-phase fraction (SPF). Biopsies from 67 patients with SCCHN were analysed for CCND1 amplification by fluorescence in situ hybridisation (FISH) and for ploidy status and SPF by flow cytometry (FCM). Twenty-one of 67 tumours (31%) showed CCND1 amplification and the frequencies differed significantly between different subsites (p = 0.01). Tumours from hypopharynx, larynx and oropharynx showed higher rates of amplification as compared to tumours from oral cavity and epipharynx.... (More)
Amplification of CCND1 (cyclin D1 gene) in squamous cell carcinoma of the head and neck (SCCHN) is correlated to poor prognosis. The purpose of this study was to investigate whether CCND1 amplification is related to different subsites and also to DNA ploidy status and S-phase fraction (SPF). Biopsies from 67 patients with SCCHN were analysed for CCND1 amplification by fluorescence in situ hybridisation (FISH) and for ploidy status and SPF by flow cytometry (FCM). Twenty-one of 67 tumours (31%) showed CCND1 amplification and the frequencies differed significantly between different subsites (p = 0.01). Tumours from hypopharynx, larynx and oropharynx showed higher rates of amplification as compared to tumours from oral cavity and epipharynx. CCND1 amplification was also associated to DNA non-diploidy and high SPF (p = 0.002 and p = 0.002, respectively). In conclusion, the rate of CCND1 amplification differed between different subsites in SCCHN and was also associated to a more aggressive tumour phenotype, as defined by DNA non-diploidy and high SPF. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flow cytometry, FISH, CCND1, SCCHN
in
Oral Oncology
volume
40
issue
6
pages
624 - 629
publisher
Elsevier
external identifiers
  • wos:000222229500010
  • scopus:1842582039
ISSN
1879-0593
DOI
10.1016/j.oraloncology.2003.12.014
language
English
LU publication?
yes
id
ad7ce62c-e43c-4bf0-a5dc-1a58c6c7f90f (old id 273734)
date added to LUP
2007-08-03 16:07:57
date last changed
2017-09-24 03:28:00
@article{ad7ce62c-e43c-4bf0-a5dc-1a58c6c7f90f,
  abstract     = {Amplification of CCND1 (cyclin D1 gene) in squamous cell carcinoma of the head and neck (SCCHN) is correlated to poor prognosis. The purpose of this study was to investigate whether CCND1 amplification is related to different subsites and also to DNA ploidy status and S-phase fraction (SPF). Biopsies from 67 patients with SCCHN were analysed for CCND1 amplification by fluorescence in situ hybridisation (FISH) and for ploidy status and SPF by flow cytometry (FCM). Twenty-one of 67 tumours (31%) showed CCND1 amplification and the frequencies differed significantly between different subsites (p = 0.01). Tumours from hypopharynx, larynx and oropharynx showed higher rates of amplification as compared to tumours from oral cavity and epipharynx. CCND1 amplification was also associated to DNA non-diploidy and high SPF (p = 0.002 and p = 0.002, respectively). In conclusion, the rate of CCND1 amplification differed between different subsites in SCCHN and was also associated to a more aggressive tumour phenotype, as defined by DNA non-diploidy and high SPF.},
  author       = {Niméus, Emma and Baldetorp, Bo and Bendahl, Pär-Ola and Rennstam, Karin and Wennerberg, Johan and Åkervall, Jan and Fernö, Mårten},
  issn         = {1879-0593},
  keyword      = {flow cytometry,FISH,CCND1,SCCHN},
  language     = {eng},
  number       = {6},
  pages        = {624--629},
  publisher    = {Elsevier},
  series       = {Oral Oncology},
  title        = {Amplification of the cyclin D1 gene is associated with tumour subsite, DNA non-diploidy and high S-phase fraction in squamous cell carcinoma of the head and neck},
  url          = {http://dx.doi.org/10.1016/j.oraloncology.2003.12.014},
  volume       = {40},
  year         = {2004},
}