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Derivation, characterization, and differentiation of human embryonic stem cells

Heins, N; Englund, MCO; Sjoblom, C; Dahl, U; Tonning, A; Bergh, C; Lindahl, A; Hanson, C and Semb, Henrik LU (2004) In Stem Cells 22(3). p.367-376
Abstract
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the... (More)
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
differentiation, pluripotency, human ES cell, human blastocyst, ICM, subcloning
in
Stem Cells
volume
22
issue
3
pages
367 - 376
publisher
AlphaMed Press
external identifiers
  • wos:000221869600016
  • pmid:15153613
  • scopus:2942591062
ISSN
1549-4918
language
English
LU publication?
no
id
8d8138ef-683f-4f56-a3e6-43cc68930286 (old id 275760)
alternative location
http://stemcells.alphamedpress.org/cgi/reprint/22/3/367.pdf
date added to LUP
2007-10-26 15:54:26
date last changed
2017-10-22 04:43:16
@article{8d8138ef-683f-4f56-a3e6-43cc68930286,
  abstract     = {The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state.},
  author       = {Heins, N and Englund, MCO and Sjoblom, C and Dahl, U and Tonning, A and Bergh, C and Lindahl, A and Hanson, C and Semb, Henrik},
  issn         = {1549-4918},
  keyword      = {differentiation,pluripotency,human ES cell,human blastocyst,ICM,subcloning},
  language     = {eng},
  number       = {3},
  pages        = {367--376},
  publisher    = {AlphaMed Press},
  series       = {Stem Cells},
  title        = {Derivation, characterization, and differentiation of human embryonic stem cells},
  volume       = {22},
  year         = {2004},
}