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Apoptotic cell death in the lactating mammary gland is enhanced by a folding variant of alpha-lactalbumin

Baltzer, A; Svanborg, Catharina LU and Jaggi, R (2004) In Cellular and Molecular Life Sciences1997-01-01+01:00 61(10). p.1221-1228
Abstract
Apoptosis is essential to eliminate secretory epithelial cells during the involution of the mammary gland. The environmental regulation of this process is however, poorly understood. This study tested the effect of HAMLET (human alpha-lactalbumin made lethal to tumor cells) on mammary cells. Plastic pellets containing HAMLET were implanted into the fourth inguinal mammary gland of lactating mice for 3 days. Exposure of mammary tissue to HAMLET resulted in morphological changes typical for apoptosis and in a stimulation of caspase-3 activity in alveolar epithelial cells near the HAMLET pellets but not more distant to the pellet or in contralateral glands. The effect was specific for HAMLET and no effects were observed when mammary glands... (More)
Apoptosis is essential to eliminate secretory epithelial cells during the involution of the mammary gland. The environmental regulation of this process is however, poorly understood. This study tested the effect of HAMLET (human alpha-lactalbumin made lethal to tumor cells) on mammary cells. Plastic pellets containing HAMLET were implanted into the fourth inguinal mammary gland of lactating mice for 3 days. Exposure of mammary tissue to HAMLET resulted in morphological changes typical for apoptosis and in a stimulation of caspase-3 activity in alveolar epithelial cells near the HAMLET pellets but not more distant to the pellet or in contralateral glands. The effect was specific for HAMLET and no effects were observed when mammary glands were exposed to native a-lactalbumin or fatty acid alone. HAMLET also induced cell death in vitro in a mouse mammary epithelial cell line. The results suggest that HAMLET can mediate apoptotic cell death in mammary gland tissue. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
involution, caspase, HAMLET, breast cancer, apoptosis, alpha-lactalbumin, protein folding
in
Cellular and Molecular Life Sciences1997-01-01+01:00
volume
61
issue
10
pages
1221 - 1228
publisher
Birkhaüser
external identifiers
  • pmid:15141307
  • wos:000221370600009
  • scopus:2542548817
ISSN
1420-9071
DOI
10.1007/s00018-004-4046-7
language
English
LU publication?
yes
id
da7dda70-5d1b-4af4-afc5-7709bb82a27b (old id 279127)
date added to LUP
2007-08-02 10:26:23
date last changed
2017-01-01 07:15:48
@article{da7dda70-5d1b-4af4-afc5-7709bb82a27b,
  abstract     = {Apoptosis is essential to eliminate secretory epithelial cells during the involution of the mammary gland. The environmental regulation of this process is however, poorly understood. This study tested the effect of HAMLET (human alpha-lactalbumin made lethal to tumor cells) on mammary cells. Plastic pellets containing HAMLET were implanted into the fourth inguinal mammary gland of lactating mice for 3 days. Exposure of mammary tissue to HAMLET resulted in morphological changes typical for apoptosis and in a stimulation of caspase-3 activity in alveolar epithelial cells near the HAMLET pellets but not more distant to the pellet or in contralateral glands. The effect was specific for HAMLET and no effects were observed when mammary glands were exposed to native a-lactalbumin or fatty acid alone. HAMLET also induced cell death in vitro in a mouse mammary epithelial cell line. The results suggest that HAMLET can mediate apoptotic cell death in mammary gland tissue.},
  author       = {Baltzer, A and Svanborg, Catharina and Jaggi, R},
  issn         = {1420-9071},
  keyword      = {involution,caspase,HAMLET,breast cancer,apoptosis,alpha-lactalbumin,protein folding},
  language     = {eng},
  number       = {10},
  pages        = {1221--1228},
  publisher    = {Birkhaüser},
  series       = {Cellular and Molecular Life Sciences1997-01-01+01:00},
  title        = {Apoptotic cell death in the lactating mammary gland is enhanced by a folding variant of alpha-lactalbumin},
  url          = {http://dx.doi.org/10.1007/s00018-004-4046-7},
  volume       = {61},
  year         = {2004},
}