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A von Willebrand factor-binding protein from Staphylococcus lugdunensis

Nilsson, M; Bjerketorp, J; Wiebensjo, A; Ljungh, Åsa LU ; Frykberg, L and Guss, B (2004) In FEMS Microbiology Letters 234(1). p.155-161
Abstract
In the present study, a phage display library covering the genome of Staphylococcus lugdunensis, was affinity-selected against von Willebrand factor (vWf). This led to the identification of a gene, vwbl, encoding a Putative cell surface protein of 2060 amino acids, denoted vWbl. The deduced protein has an overall organisation typical of staphylococcal cell Surface proteins, with an N-terminal signal peptide, and a C-terminal cell wall sorting signal. The vWf-binding part is located in repetitive domains and antibodies against vWbl or vWf can inhibit the binding. Southern blot analysis showed that vwbl was present in the 12 S. lugdunensis strains tested. (C) 2004 Federation of European Microbiological Societies.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
von Willebrand factor, display, phage, coagulase-negative staphylococci, Staphylococcus lugdunensis, adhesin, LPXTG motif
in
FEMS Microbiology Letters
volume
234
issue
1
pages
155 - 161
publisher
Elsevier
external identifiers
  • pmid:15109734
  • wos:000221218300022
  • scopus:1942470014
ISSN
1574-6968
DOI
10.1016/j.femsle.2004.03.024
language
English
LU publication?
yes
id
0aaac118-bdbe-4062-9e6e-4f11cbacc920 (old id 279441)
date added to LUP
2007-08-03 16:04:06
date last changed
2017-11-05 04:20:50
@article{0aaac118-bdbe-4062-9e6e-4f11cbacc920,
  abstract     = {In the present study, a phage display library covering the genome of Staphylococcus lugdunensis, was affinity-selected against von Willebrand factor (vWf). This led to the identification of a gene, vwbl, encoding a Putative cell surface protein of 2060 amino acids, denoted vWbl. The deduced protein has an overall organisation typical of staphylococcal cell Surface proteins, with an N-terminal signal peptide, and a C-terminal cell wall sorting signal. The vWf-binding part is located in repetitive domains and antibodies against vWbl or vWf can inhibit the binding. Southern blot analysis showed that vwbl was present in the 12 S. lugdunensis strains tested. (C) 2004 Federation of European Microbiological Societies.},
  author       = {Nilsson, M and Bjerketorp, J and Wiebensjo, A and Ljungh, Åsa and Frykberg, L and Guss, B},
  issn         = {1574-6968},
  keyword      = {von Willebrand factor,display,phage,coagulase-negative staphylococci,Staphylococcus lugdunensis,adhesin,LPXTG motif},
  language     = {eng},
  number       = {1},
  pages        = {155--161},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {A von Willebrand factor-binding protein from Staphylococcus lugdunensis},
  url          = {http://dx.doi.org/10.1016/j.femsle.2004.03.024},
  volume       = {234},
  year         = {2004},
}