Kinetic Studies of Amino Acid-Based Surfactant Binding to DNA
(2012) In The Journal of Physical Chemistry Part B 116(20). p.5831-5837- Abstract
- In this work, the binding kinetics of amino acid-based surfactants, presenting different linkers and head groups, with calf thymus (CT)-DNA was studied using stopped-flow fluorescence spectroscopy. The kinetic studies were carried out as a function of Na+ concentration and surfactant-to-DNA charge ratio. The surfactant binding on DNA took place in two consecutive steps, for which the corresponding first and second relative rate constants (k(1) and k(2)) were determined. The fast step was attributed to the surfactant binding to DNA and micelle formation in its vicinity, the slower step to DNA condensation and possible rearrangement of the surfactant aggregates. In general, both relative rate constants increase with surfactant concentration... (More)
- In this work, the binding kinetics of amino acid-based surfactants, presenting different linkers and head groups, with calf thymus (CT)-DNA was studied using stopped-flow fluorescence spectroscopy. The kinetic studies were carried out as a function of Na+ concentration and surfactant-to-DNA charge ratio. The surfactant binding on DNA took place in two consecutive steps, for which the corresponding first and second relative rate constants (k(1) and k(2)) were determined. The fast step was attributed to the surfactant binding to DNA and micelle formation in its vicinity, the slower step to DNA condensation and possible rearrangement of the surfactant aggregates. In general, both relative rate constants increase with surfactant concentration and decrease with the ionic strength of the medium. The architecture of the surfactant was found to have a significant impact on the kinetics of the DNA surfactant complexation. Surfactants with amide linkers showed larger relative rate constants than those with ester linkers. The variation of the relative rate constants with the head groups of the surfactants, alanine and proline, was found to be less obvious, being partially dependent on the surfactant concentration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2799804
- author
- Santhiya, Deenan ; Dias, Rita S. ; Dutta, Sounak ; Das, Prasanta Kumar ; Miguel, Maria G. ; Lindman, Björn LU and Maiti, Souvik
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Part B
- volume
- 116
- issue
- 20
- pages
- 5831 - 5837
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000304338300004
- scopus:84862290982
- pmid:22554103
- ISSN
- 1520-5207
- DOI
- 10.1021/jp301069t
- language
- English
- LU publication?
- yes
- id
- d6265591-ea21-427e-9695-748fe519b347 (old id 2799804)
- date added to LUP
- 2016-04-01 14:17:00
- date last changed
- 2022-01-27 23:45:33
@article{d6265591-ea21-427e-9695-748fe519b347,
abstract = {{In this work, the binding kinetics of amino acid-based surfactants, presenting different linkers and head groups, with calf thymus (CT)-DNA was studied using stopped-flow fluorescence spectroscopy. The kinetic studies were carried out as a function of Na+ concentration and surfactant-to-DNA charge ratio. The surfactant binding on DNA took place in two consecutive steps, for which the corresponding first and second relative rate constants (k(1) and k(2)) were determined. The fast step was attributed to the surfactant binding to DNA and micelle formation in its vicinity, the slower step to DNA condensation and possible rearrangement of the surfactant aggregates. In general, both relative rate constants increase with surfactant concentration and decrease with the ionic strength of the medium. The architecture of the surfactant was found to have a significant impact on the kinetics of the DNA surfactant complexation. Surfactants with amide linkers showed larger relative rate constants than those with ester linkers. The variation of the relative rate constants with the head groups of the surfactants, alanine and proline, was found to be less obvious, being partially dependent on the surfactant concentration.}},
author = {{Santhiya, Deenan and Dias, Rita S. and Dutta, Sounak and Das, Prasanta Kumar and Miguel, Maria G. and Lindman, Björn and Maiti, Souvik}},
issn = {{1520-5207}},
language = {{eng}},
number = {{20}},
pages = {{5831--5837}},
publisher = {{The American Chemical Society (ACS)}},
series = {{The Journal of Physical Chemistry Part B}},
title = {{Kinetic Studies of Amino Acid-Based Surfactant Binding to DNA}},
url = {{http://dx.doi.org/10.1021/jp301069t}},
doi = {{10.1021/jp301069t}},
volume = {{116}},
year = {{2012}},
}