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Highly sensitive detection and quantification of the secreted bacterial benevolence factor RoxP using a capacitive biosensor : A possible early detection system for oxidative skin diseases

Ertürk, Gizem LU ; Hedström, Martin LU ; Mattiasson, Bo LU ; Ruzgas, Tautgirdas LU and Lood, Rolf LU (2018) In PloS one 13(3).
Abstract

The impact of the microbiota on our health is rapidly gaining interest. While several bacteria have been associated with disease, and others being indicated as having a probiotic effect, the individual biomolecules behind these alterations are often not known. A major problem in the study of these factors in vivo is their low abundance in complex environments. We recently identified the first secreted bacterial antioxidant protein, RoxP, from the skin commensal Propionibacterium acnes, suggesting its relevance for maintaining the redox homeostasis on the skin. In order to study the effect, and prevalence, of RoxP in vivo, a capacitive biosensor with a recognition surface based on molecular imprinting was used to detect RoxP on skin in... (More)

The impact of the microbiota on our health is rapidly gaining interest. While several bacteria have been associated with disease, and others being indicated as having a probiotic effect, the individual biomolecules behind these alterations are often not known. A major problem in the study of these factors in vivo is their low abundance in complex environments. We recently identified the first secreted bacterial antioxidant protein, RoxP, from the skin commensal Propionibacterium acnes, suggesting its relevance for maintaining the redox homeostasis on the skin. In order to study the effect, and prevalence, of RoxP in vivo, a capacitive biosensor with a recognition surface based on molecular imprinting was used to detect RoxP on skin in vivo. In vitro analyses demonstrated the ability to detect and quantify RoxP in a concentration range of 1 x 10-13 M to 1 x 10-8 M from human skin swabs; with a limit of detection of 2.5 x 10-19 M in buffer systems. Further, the biosensor was highly selective, not responding to any other secreted protein from P. acnes. Thus, it was possible to demonstrate the presence, and quantity, of RoxP on human skin. Therefore, the developed biosensor is a very promising tool for the detection of RoxP from clinical samples, offering a rapid, cost-effective and sensitive means of detecting low-abundant bacterial proteins in vivo in complex milieus.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PloS one
volume
13
issue
3
publisher
Public Library of Science
external identifiers
  • scopus:85042708634
ISSN
1932-6203
DOI
10.1371/journal.pone.0193754
language
English
LU publication?
yes
id
27d8c56a-8452-482d-942f-63a9fce86dc4
date added to LUP
2018-03-05 08:27:22
date last changed
2018-06-05 03:00:11
@article{27d8c56a-8452-482d-942f-63a9fce86dc4,
  abstract     = {<p>The impact of the microbiota on our health is rapidly gaining interest. While several bacteria have been associated with disease, and others being indicated as having a probiotic effect, the individual biomolecules behind these alterations are often not known. A major problem in the study of these factors in vivo is their low abundance in complex environments. We recently identified the first secreted bacterial antioxidant protein, RoxP, from the skin commensal Propionibacterium acnes, suggesting its relevance for maintaining the redox homeostasis on the skin. In order to study the effect, and prevalence, of RoxP in vivo, a capacitive biosensor with a recognition surface based on molecular imprinting was used to detect RoxP on skin in vivo. In vitro analyses demonstrated the ability to detect and quantify RoxP in a concentration range of 1 x 10-13 M to 1 x 10-8 M from human skin swabs; with a limit of detection of 2.5 x 10-19 M in buffer systems. Further, the biosensor was highly selective, not responding to any other secreted protein from P. acnes. Thus, it was possible to demonstrate the presence, and quantity, of RoxP on human skin. Therefore, the developed biosensor is a very promising tool for the detection of RoxP from clinical samples, offering a rapid, cost-effective and sensitive means of detecting low-abundant bacterial proteins in vivo in complex milieus.</p>},
  articleno    = {e0193754},
  author       = {Ertürk, Gizem and Hedström, Martin and Mattiasson, Bo and Ruzgas, Tautgirdas and Lood, Rolf},
  issn         = {1932-6203},
  language     = {eng},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PloS one},
  title        = {Highly sensitive detection and quantification of the secreted bacterial benevolence factor RoxP using a capacitive biosensor : A possible early detection system for oxidative skin diseases},
  url          = {http://dx.doi.org/10.1371/journal.pone.0193754},
  volume       = {13},
  year         = {2018},
}