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Ultrastructural alteration of tape-stripped normal human skin after photodynamic therapy

Bartosik, Jacek LU ; Stender, IM; Kobayasi, T and Agren, MS (2004) In EJD. European Journal of Dermatology 14(2). p.91-95
Abstract
The effect of photodynamic therapy on tape-stripped normal human skin was explored ultrastructurally. Back skin areas of 3 healthy Caucasian volunteers, 2 men and 1 woman, were tape-stripped 10 consecutive times followed by topical treatment with 5-aminolevulinic acid (20%, w/w) for 4 hours under occlusion (Tegaderm(R)). Then the areas were irradiated for 30 minutes with Waldman PDT 1200 lamp (570-650 nm) with a total dose of 70 J/cm(2). Full-thickness punch biopsies were taken immediately after irradiation, and at 3 and 24 hours for electron microscopy examination. Photodynamic therapy caused morphological alterations mainly in the epidermis. Keratinocytes became oedematous and tonofilament bundles were split, but desmosomes remained... (More)
The effect of photodynamic therapy on tape-stripped normal human skin was explored ultrastructurally. Back skin areas of 3 healthy Caucasian volunteers, 2 men and 1 woman, were tape-stripped 10 consecutive times followed by topical treatment with 5-aminolevulinic acid (20%, w/w) for 4 hours under occlusion (Tegaderm(R)). Then the areas were irradiated for 30 minutes with Waldman PDT 1200 lamp (570-650 nm) with a total dose of 70 J/cm(2). Full-thickness punch biopsies were taken immediately after irradiation, and at 3 and 24 hours for electron microscopy examination. Photodynamic therapy caused morphological alterations mainly in the epidermis. Keratinocytes became oedematous and tonofilament bundles were split, but desmosomes remained normal. Many keratinocytes contained large intracellular vacuoles and extremely electronlucent cytoplasm implying cell damage. Although the majority of Langerhans cells were unaffected isolated Langerhans cells became apoptotic at 3 hours. The melanocytes preserved their normal morphology. The epidermal alterations recovered 24 hours after the irradiation. Inflammatory cell infiltrates were evident at 3 and 24 hours but no other dermal changes were observed. In conclusion, photodynamic therapy with 5-aminolevulinic acid affects mainly keratinocytes and can trigger apoptosis in Langerhans cells while melanocytes appear refractory, at least morphologically, to photodynamic therapy. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
keratinocytes, melanocytes, 5-aminolevulinic acid, Langerhans cells, ultrastructure, apoptosis
in
EJD. European Journal of Dermatology
volume
14
issue
2
pages
91 - 95
publisher
John Libbey Eurotext
external identifiers
  • wos:000221027700003
  • scopus:1942440898
ISSN
1167-1122
language
English
LU publication?
yes
id
d71ec3d2-96d9-4bca-9ad6-dbac4529d230 (old id 280477)
alternative location
http://www.jle.com/fr/revues/medecine/ejd/e-docs/00/04/00/6B/article.md?type=text.html
date added to LUP
2007-10-16 16:41:36
date last changed
2017-01-01 06:49:05
@article{d71ec3d2-96d9-4bca-9ad6-dbac4529d230,
  abstract     = {The effect of photodynamic therapy on tape-stripped normal human skin was explored ultrastructurally. Back skin areas of 3 healthy Caucasian volunteers, 2 men and 1 woman, were tape-stripped 10 consecutive times followed by topical treatment with 5-aminolevulinic acid (20%, w/w) for 4 hours under occlusion (Tegaderm(R)). Then the areas were irradiated for 30 minutes with Waldman PDT 1200 lamp (570-650 nm) with a total dose of 70 J/cm(2). Full-thickness punch biopsies were taken immediately after irradiation, and at 3 and 24 hours for electron microscopy examination. Photodynamic therapy caused morphological alterations mainly in the epidermis. Keratinocytes became oedematous and tonofilament bundles were split, but desmosomes remained normal. Many keratinocytes contained large intracellular vacuoles and extremely electronlucent cytoplasm implying cell damage. Although the majority of Langerhans cells were unaffected isolated Langerhans cells became apoptotic at 3 hours. The melanocytes preserved their normal morphology. The epidermal alterations recovered 24 hours after the irradiation. Inflammatory cell infiltrates were evident at 3 and 24 hours but no other dermal changes were observed. In conclusion, photodynamic therapy with 5-aminolevulinic acid affects mainly keratinocytes and can trigger apoptosis in Langerhans cells while melanocytes appear refractory, at least morphologically, to photodynamic therapy.},
  author       = {Bartosik, Jacek and Stender, IM and Kobayasi, T and Agren, MS},
  issn         = {1167-1122},
  keyword      = {keratinocytes,melanocytes,5-aminolevulinic acid,Langerhans cells,ultrastructure,apoptosis},
  language     = {eng},
  number       = {2},
  pages        = {91--95},
  publisher    = {John Libbey Eurotext},
  series       = {EJD. European Journal of Dermatology},
  title        = {Ultrastructural alteration of tape-stripped normal human skin after photodynamic therapy},
  volume       = {14},
  year         = {2004},
}