Modulation of antigen-induced responses by serotonin and prostaglandin E(2) via EP(1) and EP(4) receptors in the peripheral rat lung.
Larsson Callerfelt, Anna-Karin LU
; Dahlén, Sven-Erik
; Kühl, Anna-Rebekka
; Lex, Dennis
; Uhlig, Stefan
and Martin, Christian
(2013)
In European Journal of Pharmacology
699(1-3).
p.141-149
- Abstract
- The cyclooxygenase (COX) pathway and prostanoids may critically contribute to the early allergic airway response. In the rat lung, serotonin (5-HT) is a major mediator of antigen-induced contractions. The aim of this study was therefore to examine the relative role of the COX pathway and serotonin for antigen-induced contractions in the rat lung. Airway responses were studied in rat precision-cut lung slices (PCLS). Lung slices were stimulated with ovalbumin or serotonin after pretreatment with COX inhibitors or specific TP or EP receptor antagonists. Changes in airway size (contractions/relaxations) were measured by a digital video camera. The supernatants were analysed for changes in prostaglandin and serotonin release. Airway... (More)
- The cyclooxygenase (COX) pathway and prostanoids may critically contribute to the early allergic airway response. In the rat lung, serotonin (5-HT) is a major mediator of antigen-induced contractions. The aim of this study was therefore to examine the relative role of the COX pathway and serotonin for antigen-induced contractions in the rat lung. Airway responses were studied in rat precision-cut lung slices (PCLS). Lung slices were stimulated with ovalbumin or serotonin after pretreatment with COX inhibitors or specific TP or EP receptor antagonists. Changes in airway size (contractions/relaxations) were measured by a digital video camera. The supernatants were analysed for changes in prostaglandin and serotonin release. Airway contractions to ovalbumin were attenuated by the unselective COX inhibitor indomethacin, the selective COX-1 inhibitor FR-122047 and COX-2 inhibitor celecoxib. The EP(1) receptor antagonist ONO-8713 reduced the contractions, whereas the EP(4) receptor antagonist L-161,982 significantly increased the contractile response to ovalbumin. The 5-HT(2A) receptor antagonist ketanserin completely inhibited the ovalbumin-induced contractions. The different COX inhibitors decreased the production of prostaglandins but did not affect the synthesis of serotonin. The serotonin-induced bronchoconstriction was attenuated by celecoxib and ONO-8713, but not by methacholine. Taken together, our data indicate that PGE(2) is the main prostanoid involved in the early allergic airway response in the rat lung. PGE(2) appears to act both as a primary mediator of antigen-induced airway contraction via the EP(4) receptor and as a downstream modulator of serotonin-induced bronchoconstriction via the EP(1) receptor. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3347488
- author
- Larsson Callerfelt, Anna-Karin
LU
; Dahlén, Sven-Erik
; Kühl, Anna-Rebekka
; Lex, Dennis
; Uhlig, Stefan
and Martin, Christian
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Contraction, Airway smooth muscle, Ovalbumin, Precision-cut lung slices, Prostaglandins, Serotonin
- in
- European Journal of Pharmacology
- volume
- 699
- issue
- 1-3
- pages
- 141 - 149
- publisher
- Elsevier
- external identifiers
-
- wos:000314659600020
- pmid:23220160
- scopus:84871940768
- pmid:23220160
- ISSN
- 1879-0712
- DOI
- 10.1016/j.ejphar.2012.11.039
- language
- English
- LU publication?
- yes
- id
- 28157f97-09a0-4c3f-9eb4-5fd46ea7346c (old id 3347488)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23220160?dopt=Abstract
- date added to LUP
- 2016-04-01 09:57:58
- date last changed
- 2022-04-27 17:17:54
@article{28157f97-09a0-4c3f-9eb4-5fd46ea7346c,
abstract = {{The cyclooxygenase (COX) pathway and prostanoids may critically contribute to the early allergic airway response. In the rat lung, serotonin (5-HT) is a major mediator of antigen-induced contractions. The aim of this study was therefore to examine the relative role of the COX pathway and serotonin for antigen-induced contractions in the rat lung. Airway responses were studied in rat precision-cut lung slices (PCLS). Lung slices were stimulated with ovalbumin or serotonin after pretreatment with COX inhibitors or specific TP or EP receptor antagonists. Changes in airway size (contractions/relaxations) were measured by a digital video camera. The supernatants were analysed for changes in prostaglandin and serotonin release. Airway contractions to ovalbumin were attenuated by the unselective COX inhibitor indomethacin, the selective COX-1 inhibitor FR-122047 and COX-2 inhibitor celecoxib. The EP(1) receptor antagonist ONO-8713 reduced the contractions, whereas the EP(4) receptor antagonist L-161,982 significantly increased the contractile response to ovalbumin. The 5-HT(2A) receptor antagonist ketanserin completely inhibited the ovalbumin-induced contractions. The different COX inhibitors decreased the production of prostaglandins but did not affect the synthesis of serotonin. The serotonin-induced bronchoconstriction was attenuated by celecoxib and ONO-8713, but not by methacholine. Taken together, our data indicate that PGE(2) is the main prostanoid involved in the early allergic airway response in the rat lung. PGE(2) appears to act both as a primary mediator of antigen-induced airway contraction via the EP(4) receptor and as a downstream modulator of serotonin-induced bronchoconstriction via the EP(1) receptor.}},
author = {{Larsson Callerfelt, Anna-Karin and Dahlén, Sven-Erik and Kühl, Anna-Rebekka and Lex, Dennis and Uhlig, Stefan and Martin, Christian}},
issn = {{1879-0712}},
keywords = {{Contraction; Airway smooth muscle; Ovalbumin; Precision-cut lung slices; Prostaglandins; Serotonin}},
language = {{eng}},
number = {{1-3}},
pages = {{141--149}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmacology}},
title = {{Modulation of antigen-induced responses by serotonin and prostaglandin E(2) via EP(1) and EP(4) receptors in the peripheral rat lung.}},
url = {{https://lup.lub.lu.se/search/files/1431642/3615282.pdf}},
doi = {{10.1016/j.ejphar.2012.11.039}},
volume = {{699}},
year = {{2013}},
}