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Complement activation by both classical and alternative pathways is critical for the effector phase of arthritis

Hietala, MA; Nandakumar, KS; Persson, L; Fahlen, S; Holmdahl, Rikard LU and Pekna, M (2004) In European Journal of Immunology 34(4). p.1208-1216
Abstract
To analyze the role of the classical and alternative pathways of complement activation in the effector phase of arthritis, we have induced arthritis in C3- and factor B (FB)-deficient (C3(-/-) and FB-/-) DBA/1J mice using well-defined monoclonal IgG2b and IgG2a antibodies to type II collagen. In control DBA/1J mice, severe swelling of the joints, destruction of cartilage and erosion of bone developed very rapidly with a 100% incidence and a peak on days 7-10. Although 75% of C3(-/-) mice developed arthritis, the clinical severity was very mild and the onset was delayed. Severity of arthritis in FB-/- mice ranked intermediate in comparison with C3(-/-) and control mice with an incidence of 100%. Immunohistochemical analysis of the inflamed... (More)
To analyze the role of the classical and alternative pathways of complement activation in the effector phase of arthritis, we have induced arthritis in C3- and factor B (FB)-deficient (C3(-/-) and FB-/-) DBA/1J mice using well-defined monoclonal IgG2b and IgG2a antibodies to type II collagen. In control DBA/1J mice, severe swelling of the joints, destruction of cartilage and erosion of bone developed very rapidly with a 100% incidence and a peak on days 7-10. Although 75% of C3(-/-) mice developed arthritis, the clinical severity was very mild and the onset was delayed. Severity of arthritis in FB-/- mice ranked intermediate in comparison with C3(-/-) and control mice with an incidence of 100%. Immunohistochemical analysis of the inflamed joints demonstrated substantial reduction in macrophage and neutrophilic leukocyte infiltration in both C3(-/-) and FB-/- mice, thereby confirming the clinical findings. We conclude that both the classical and the alternative pathways of complement activation are involved in the effector phase of arthritis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
rodent, complement, rheumatoid arthritis, transgenic, knockout
in
European Journal of Immunology
volume
34
issue
4
pages
1208 - 1216
publisher
John Wiley & Sons
external identifiers
  • wos:000220834600032
  • pmid:15048732
  • scopus:3843142994
ISSN
1521-4141
DOI
10.1002/eji.200424895
language
English
LU publication?
yes
id
79a17eb9-aa16-40a3-9a19-2c747190a333 (old id 281654)
date added to LUP
2007-10-30 12:06:24
date last changed
2017-03-26 03:36:56
@article{79a17eb9-aa16-40a3-9a19-2c747190a333,
  abstract     = {To analyze the role of the classical and alternative pathways of complement activation in the effector phase of arthritis, we have induced arthritis in C3- and factor B (FB)-deficient (C3(-/-) and FB-/-) DBA/1J mice using well-defined monoclonal IgG2b and IgG2a antibodies to type II collagen. In control DBA/1J mice, severe swelling of the joints, destruction of cartilage and erosion of bone developed very rapidly with a 100% incidence and a peak on days 7-10. Although 75% of C3(-/-) mice developed arthritis, the clinical severity was very mild and the onset was delayed. Severity of arthritis in FB-/- mice ranked intermediate in comparison with C3(-/-) and control mice with an incidence of 100%. Immunohistochemical analysis of the inflamed joints demonstrated substantial reduction in macrophage and neutrophilic leukocyte infiltration in both C3(-/-) and FB-/- mice, thereby confirming the clinical findings. We conclude that both the classical and the alternative pathways of complement activation are involved in the effector phase of arthritis.},
  author       = {Hietala, MA and Nandakumar, KS and Persson, L and Fahlen, S and Holmdahl, Rikard and Pekna, M},
  issn         = {1521-4141},
  keyword      = {rodent,complement,rheumatoid arthritis,transgenic,knockout},
  language     = {eng},
  number       = {4},
  pages        = {1208--1216},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {Complement activation by both classical and alternative pathways is critical for the effector phase of arthritis},
  url          = {http://dx.doi.org/10.1002/eji.200424895},
  volume       = {34},
  year         = {2004},
}